We narrowed to 10,484 results for: poly

1. pcDNA3-PB2-3x-FLAG-B/BRISBANE/60/2008

   Plasmid

   #187916

   Purpose

   Expresses C terminal 3xFLAG tagged PB2 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

   Depositor

   Arindam Mondal

   Article

   Kedia et al Front Microbiol. 2022 May 25;13:868367.

   Insert

   polymerase basic protein 2
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Promoter

   Available since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CAG-SuperNovaGreen-T2A-dTomato

   Plasmid

   #193798

   Purpose

   Expresses SuperNova Green in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

   Depositor

   Xiao Wang

   Article

   Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

   Insert

   SuperNova Green
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-PB1-3x-FLAG-B/BRISBANE/60/2008

   Plasmid

   #187918

   Purpose

   Expresses C terminal 3xFLAG tagged PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

   Depositor

   Arindam Mondal

   Article

   Kedia et al Front Microbiol. 2022 May 25;13:868367.

   Insert

   polymerase basic protein 1
   

   Use

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   CMV Promoter

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWzyE-distrepII

   Plasmid

   #196667

   Purpose

   Expression of C-terminally distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli

   Depositor

   James Naismith

   Article

   Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

   Insert

   WzyE
   

   Use

   Tags

   TEV-cleavable di-strep tag II

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pK1E-RNAP

   Plasmid

   #48144

   Purpose

   Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylA

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

   Insert

   rna polymerase K1E
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti.puro.MCV.ER.RAZ.2

   Plasmid

   #114382

   Purpose

   This lentiviral plasmid expresses MCV tumor T antigens (truncated LT and small T) using the viral NCCR (non-coding control region). The sequence was amplifeied from MCV.R17a and truncated LT=428 aa.

   Depositor

   Sudhir Krishna, Reety Arora

   Article

   Arora et al Microb Pathog. 2019 Dec;137:103778. doi:

   Insert

   MCV NCCR and tumor T antigen regions
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MCV NCCR (viral own promoter)

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-mCherry-T2A-miniSOG-C-Far

   Plasmid

   #193915

   Purpose

   Expresses miniSOG targeted to the plasma membrane of neurons, joined by a T2A cleavage site with mCherry; for AAV production

   Depositor

   Xiao Wang

   Article

   Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

   Insert

   miniSOG
   

   Use

   AAV

   Tags

   Farnesylation signal sequence and Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. KA501: pMVP (L3-L2) pA; CMV::TETa-pA

   Plasmid

   #121801

   Purpose

   pMVP L3-L2 entry plasmid, contains polyA + CMV-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds CMV-driven TETa downstream of gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   polyA + CMV::TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. KO701: pMVP (L3-L2) HA tag + pA; EF1a::TETa-pA

   Plasmid

   #121806

   Purpose

   pMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven TETa

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   HA epitope tag-polyA + EF1a::TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHis-WzzE

    Plasmid

    #196661

    Purpose

    Expression of N-terminally His6-tagged WzzE from Pectobacterium atrosepticum in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    WzzE
    

    Use

    Tags

    TEV-cleavable His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGST-WzzE

    Plasmid

    #196662

    Purpose

    Expression of N-terminally GST-tagged WzzE from Pectobacterium atrosepticum in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    WzzE
    

    Use

    Tags

    TEV-cleavable GST

    Expression

    Bacterial

    Mutation

    Valine insertion at position 2.

    Promoter

    T7

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHis-mCherry-WzzE

    Plasmid

    #196663

    Purpose

    Expression of N-terminally His6-mCherry-tagged WzzE from Pectobacterium atrosepticum in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    WzzE
    

    Use

    Tags

    His and mCherry

    Expression

    Bacterial

    Mutation

    Valine insertion at position 2 of WzzE.

    Promoter

    T7

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPglL-strep-mCherry

    Plasmid

    #196664

    Purpose

    Expression of C-terminally distrep tag II-mCherry-tagged PglL from Neisseria meningitidis in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    PglL
    

    Use

    Tags

    TEV-cleavable distrep tag II and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPglL-strep-mClover3

    Plasmid

    #196665

    Purpose

    Expression of C-terminally distrep tag II-mClover3-tagged PglL from Neisseria meningitidis in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    PglL
    

    Use

    Tags

    TEV-cleavable di-strep tag II and mClover3

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWzyE-GST-distrepII

    Plasmid

    #196666

    Purpose

    Expression of C-terminally GST-distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    WzyE
    

    Use

    Tags

    GST and TEV-cleavable di-strep tag II

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pWzyE-strep-mClover3

    Plasmid

    #196668

    Purpose

    Expression of C-terminally distrep tag II-mClover3-tagged WzyE from Pectobacterium atrosepticum in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    WzyE
    

    Use

    Tags

    TEV-cleavable di-strep tag II and mClover3

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV6-AN-HA_aa 1-449 POLH

    Plasmid

    #201583

    Purpose

    Expresses the catalytic domain (aa 1-449) of human DNA polymerase eta with a HA tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    POLH (POLH Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Expresses amino acids 1-449 of DNA polymerase eta

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. L2_NOP1gRNA-Cas9-CsA

    Plasmid

    #136139

    Purpose

    Plasmid with NOP1-gRNA to tranform Mp as CRISPR control. Contains Cas9 and HygR

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-35Sx2:HygR p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. wtVP1 only

    Plasmid

    #166673

    Purpose

    Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1, with GAL1 promoter. Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Insert

    Wild-type Murine polyomavirus VP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. KO602: pMVP (L3-L2) HA tag + pA; CMV::TETa-pA

    Plasmid

    #121802

    Purpose

    pMVP L3-L2 entry plasmid, contains HA tag-polyA + CMV-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream CMV-driven TETa

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    HA epitope tag-polyA + CMV::TETa-polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge