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pME_G_0_1_011_5C3SF

Plasmid

#235791

Purpose

5' Connector 3 Short Forward in the M1 position used to dictate position for lvl2 assembly

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Connector 3 Short Forward

Use

Synthetic Biology

Mutation

none

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_1_022_5C4SF

Plasmid

#235792

Purpose

5' Connector 4 Short Forward in the M1 position used to dictate position for lvl2 assembly

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Connector 4 Short Forward

Use

Synthetic Biology

Mutation

none

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_1_014_5C5SF

Plasmid

#235793

Purpose

5' Connector 5 Short Forward in the M1 position used to dictate position for lvl2 assembly

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Connector 5 Short Forward

Use

Synthetic Biology

Mutation

none

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_1_001_psbH_5'Hom_Cr

Plasmid

#235784

Purpose

5' Homology psbH in the M1 position used for genome integration at the psbH locus

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Homology region of the psbH locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_1_004_psbA_5'Hom_Cr

Plasmid

#235785

Purpose

5' Homology psbA in the M1 position used for genome integration at the psbA locus

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Homology region of the psbA locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_1_007_petB_5'Hom_cr

Plasmid

#235787

Purpose

5' Homology petB in the M1 position used for genome integration at the petB locus

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Homology region of the petB locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_1_005_5C1SF

Plasmid

#235789

Purpose

5' Connector 1 Short Forward in the M1 position used to dictate position for lvl2 assembly

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Connector 1 Short Forward

Use

Synthetic Biology

Mutation

none

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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ESR1-luciferase

Plasmid

#113351

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of ESR1 gene

Depositor

Ralf Jauch

Publication

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

ESR1-enhancer (ESR1 Human)

Use

Luciferase

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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STARR-seq luciferase validation vector_mP_empty

Plasmid

#99298

Purpose

Luciferase validation vector; empty vector without insert to determine baseline

Depositor

Alexander Stark

Publication

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Luciferase; Starr-seq luciferase validation vector

Expression

Mammalian

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pReporter_7

Plasmid

#60567

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Publication

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_7

Expression

Bacterial

Promoter

BBa_J23119

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HtrA2-FLAG S142D

Plasmid

#15939

Depositor

L. Miguel Martins

Publication

Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

Insert

HtrA2 (HTRA2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S142D

Available since

Nov. 5, 2007

Availability

Academic Institutions and Nonprofits only
pMOS009: pLX304-QuasAr2

Plasmid

#62985

Purpose

Lentivirus production of QuasAr2 fluorescent reporter

Depositor

Adam Cohen

Publication

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

QuasAr2-mOrange

Use

Gateway Cloning and Lentiviral

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-PPO-Venus (AAV8)

Viral prep

#139505-AAV8

Purpose

Ready-to-use AAV8 particles produced from pAAV-Ef1a-DIO-PPO-Venus (#139505). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO-PPO-Venus plasmid DNA. EF1a-driven, Cre-dependent expression of Venus-tagged parapinopsin for spatiotemporal control of inhibitory GPCR signaling cascades. These AAV preparations are suitable purity for injection into animals.

Depositor

Michael Bruchas

Publication

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Promoter

EF1a

Tags

Venus (Cre-dependent)

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only
pPUR-mU6-sgRNA-2XPP7

Plasmid

#194504

Purpose

Empty backbone for sgRNA-2XPP7 in CRIPSR FISHer system

Depositor

Chunqing Song

Publication

Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCfB3052(gRNA X-4, XI-3, XII-5)

Plasmid

#73294

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-4, XI-3, and XII-5

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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psiCHECK2-E2F1 3'UTR

Plasmid

#29468

Depositor

Judy Lieberman

Publication

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

E2F1 3'UTR (E2F1 Human)

Expression

Mammalian

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
psiCHECK2-p27Kip1 3'UTR

Plasmid

#29476

Depositor

Judy Lieberman

Publication

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

p27 Kip1 3'UTR (CDKN1B Human)

Expression

Mammalian

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
psiCHECK2-BRCA1 3'UTR

Plasmid

#29477

Depositor

Judy Lieberman

Publication

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

BRCA1 3'UTR (BRCA1 Human)

Expression

Mammalian

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
xCas9

Plasmid

#138565

Purpose

Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Publication

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

xCas9

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Promoter

EFS

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-PuRRRE

Plasmid

#208327

Purpose

C-terminal PuRRRE-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Publication

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-PuRRRE

Tags

PuRRRE

Expression

Mammalian

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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