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pCINeo-hFX-HA

Plasmid

#14976

Depositor

Michael Ristow

Article

Schulz et al J Biol Chem. 2006 Jan 13. 281(2):977-81.

Insert

human frataxin cDNA (FXN Human)

Tags

AU1 and HA

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
SI16-Kit a

Plasmid

#58948

Purpose

expresses recombinant mouse anti-Kit a receptor (zebrafish) IgG1 antibody in mammalian cells

Depositor

Gavin Wright

Article

Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

Insert

Kit receptor a mouse monoclonal antibody (kita Zebrafish)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pLPC-mCh-ACTB-P2A-EGFP

Plasmid

#165158

Purpose

Expresses an ACTB gene fragment with P2A, flanked by mCherry and EGFP, in Mammalian cells

Depositor

Taekjip Ha

Article

Zou et al Mol Cell. 2021 Apr 1;81(7):1553-1565.e8.

Insert

mCherry-ACTB-P2A-EGFP (ACTB Human)

Use

Retroviral

Tags

EGFP and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-Beclin1 (1-242)

Plasmid

#24401

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Tags

His

Expression

Bacterial

Mutation

Contains amino acids 1-242 only.

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pSirenRetroQ(puro)-shEgr3

Plasmid

#108115

Purpose

pSIREN-RetroQ vector containing shRNA sequence to Egr3

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

shRNA to Egr3 (Egr3 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

U6

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Actin-7

Plasmid

#55399

Purpose

Localization: Actin, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Actin (ACTB Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
MDH1-miR-181a1-Dusp6

Plasmid

#17849

Depositor

Chang-Zheng Chen

Article

Li et al Cell. 2007 Apr 6. 129(1):147-61.

Insert

Dusp6-EGFP (Dusp6 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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pISH-Hopx

Plasmid

#74347

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Hopx. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

HOP homeobox (Hopx Mouse)

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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pBV-Luc/Del-7

Plasmid

#14968

Depositor

Joan Massague

Article

Chen et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

Insert

c-myc promoter (-26/+334) (MYC Human)

Use

Luciferase

Expression

Mammalian

Mutation

-26/+334 (relative to the P2 transcription initia…

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pMM2-rPMCA3b

Plasmid

#47763

Purpose

mammalian expression of rat PMCA3b

Depositor

John Penniston, Emanuel Strehler

Article

Enyedi et al J Biol Chem. 1997 Oct 31;272(44):27525-8

Insert

PMCA3b (Atp2b3 Rat)

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only
pHH0103_CLTC_Clathrin

Plasmid

#109997

Purpose

Protein expression and purification of CLTC_Clathrin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

CLTC_Clathrin (CLTC Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBV-Luc/Frag-F

Plasmid

#14053

Depositor

Joan Massague

Article

Chen et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

Insert

c-myc promoter (-109/-3) (MYC Human)

Use

Luciferase

Expression

Mammalian

Mutation

-109/-3 (relative to the P2 transcription initiat…

Available Since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-MtPylRS (human-opti)

Plasmid

#164080

Purpose

To express a Methanosarcina thermophila derived PylRS in mammalian cells for genetic code expansion

Depositor

Tao Liu

Article

Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

Insert

MtPylRS-478(human-opti)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-MfBulKRS (human-opti)

Plasmid

#164196

Purpose

To express a Methanosarcina flavescens derived BulKRS in mammalian cells for genetic code expansion

Depositor

Tao Liu

Article

Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

Insert

Mf BulKRS(human-opti)

Use

Synthetic Biology

Expression

Mammalian

Mutation

Y269A;Y347F

Promoter

CMV

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIM5alpha(human)(R332P)

Plasmid

#79037

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(human R332P) (TRIM5 Human)

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Changed Arg 332 to Pro

Promoter

polyhedrin

Available Since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-Climp63(1–301)

Plasmid

#186956

Purpose

Expresses mCherry-Climp63 with partial luminal domain

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

Ckap4 (Ckap4 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

Only remain partial luminal domain

Promoter

CMV Promoter

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-Climp63(1–437)

Plasmid

#186958

Purpose

Expresses mCherry-Climp63 with partial luminal domain

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

Ckap4 (Ckap4 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

Only remain partial luminal domain

Promoter

CMV Promoter

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-CUL1 L756A/I757A

Plasmid

#19938

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

cullin 1 L756A/I757A (CUL1 Human)

Tags

HA2

Expression

Mammalian

Mutation

Mutation of two adjacent hydrophobic residues, L…

Available Since

April 17, 2009

Availability

Academic Institutions and Nonprofits only
alpha1Ddelta-G12-FRB

Plasmid

#87454

Purpose

rapamycin-mediated heterodimerization between IQV and PCRD/DCRD from C-terminal of LTCCs

Depositor

Xiaodong Liu

Article

Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

Insert

a1D short variants (Cacna1d Rat)

Tags

FRB

Expression

Mammalian

Mutation

delete KYPAKNTTIALQMLERML

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMiniT:myc-GluA1 TKIT donor

Plasmid

#169419

Purpose

Donor DNA for TKIT knockin of Myc to GluA1 in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Myc (Myc Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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