We narrowed to 59,421 results for: cloning

1. Hs.KRAS4a G13D

   Plasmid

   #83170

   Purpose

   Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G13D mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   G13D

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti_TMv2

   Plasmid

   #131761

   Purpose

   frameshift reporter for barcode retrieval

   Depositor

   Paul Blainey

   Article

   Feldman et al BMC Biol. 2020 Nov 24;18(1):177. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNH082

   Plasmid

   #42165

   Depositor

   Colin Dolphin

   Article

   Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

   Insert

   mTFP1
   

   Expression

   Worm

   Available Since

   Feb. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR221-Firefly

   Plasmid

   #79495

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Firefly Luciferase CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   Firefly Luciferase
   

   Use

   Multisite gateway entry clone

   Promoter

   no Promoter

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMB30

   Plasmid

   #122247

   Purpose

   Lentivirus delivery for stable expression of Lb crRNA, has LbDR; Cloning vector for expression of LbCas12a crRNA. It contains BsmBI site for cloning.

   Depositor

   Darjus Tschaharganeh

   Article

   Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR221-sGFP

   Plasmid

   #79508

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Super GFP CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   superGFP
   

   Use

   Multisite gateway entry clone

   Tags

   6xHis

   Promoter

   no Promoter

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIIS18-SapI

   Plasmid

   #128599

   Purpose

   Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

   Depositor

   Tohru Suzuki

   Article

   Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIIS18-BsmBI

   Plasmid

   #128648

   Purpose

   Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

   Depositor

   Tohru Suzuki

   Article

   Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Hs.KRAS4a Q61L

   Plasmid

   #83171

   Purpose

   Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), Q61L mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   Q61L

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. p3E-mKate2-HA no-pA

    Plasmid

    #80810

    Purpose

    hemagglutinin (HA) epitope fused to C-terminus of mKate2 without pA

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    mKateMyc p3E
    

    Expression

    Mammalian

    Promoter

    HA

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. Hs.KRAS4b A146T

    Plasmid

    #83150

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), A146T mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    A146T

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. Hs.KRAS4a Q61R

    Plasmid

    #83172

    Purpose

    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), Q61R mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    Q61R

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSGV Rh Acceptor PGK-NGFR

    Plasmid

    #64272

    Purpose

    Retroviral golden gate cloning vector for expression of functional Rhesus T-Cell Receptor genes with NGFR

    Depositor

    David Ott

    Article

    Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

    Type

    Empty backbone

    Use

    Retroviral

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR-L4-pTF-L1R

    Plasmid

    #45954

    Depositor

    Patrick Salmon

    Article

    Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

    Insert

    pTF promoter
    

    Use

    Gateway donor vector

    Promoter

    TET optimized

    Available Since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. Hs.KRAS4b G12A

    Plasmid

    #83142

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12A mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    G12A

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNH013

    Plasmid

    #42147

    Depositor

    Colin Dolphin

    Article

    Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

    Insert

    mTFP1
    

    Use

    Synthetic Biology

    Available Since

    Feb. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. p3E-CFP-HA no-pA

    Plasmid

    #82591

    Purpose

    3' entry vector containing HA epitope fused to c-terminus of CFP without pA for imaging, epitope labeling/purification

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    ECFP-HA (no pA)
    

    Promoter

    None

    Available Since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. Hs.KRAS4b C185S

    Plasmid

    #83139

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), C185S mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    C185S

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. p3E-mCherry-HA no-pA

    Plasmid

    #82592

    Purpose

    3' entry vector with HA epitope fused to C-terminus of mCherry without pA for imaging, epitope labeling/purification

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    mCherry-HA (no pA)
    

    Promoter

    None

    Available Since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEN_TT miRc2 3xFLAG

    Plasmid

    #83274

    Purpose

    Empty entry vector for cloning inducible mammalian expression and an in-frame N-terminal 3xFLAG

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Type

    Empty backbone

    Use

    Entry vector for gateway cloning

    Tags

    3xFLAG

    Promoter

    TRE tight

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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