We narrowed to 66,338 results for: nin

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pHAGE-3FLAG-EEA1

Plasmid

#176491

Purpose

For lentiviral transduction of 3FLAG-EEA1

Depositor

Wade Harper

Publication

Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

Insert

EEA1 (EEA1 Human)

Use

Lentiviral

Tags

3xFLAG

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-SPOTlight-U2GCR

Plasmid

#164819

Purpose

Reporter to measure ISR state-dependent protein synthesis

Depositor

Nicole Calakos

Publication

Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

Insert

SPOTlight-U2GCR

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti6-CENP-A-mCherry

Plasmid

#89767

Purpose

Lentivirus destination vector for stable expression of mCherry-tagged kinetochore marker CENP-A

Depositor

Torsten Wittmann

Publication

Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

Insert

CENP-A (CENPA Human)

Use

Lentiviral

Tags

3xHA and mCherry

Expression

Mammalian

Promoter

CMV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pDEST26-hOPTN

Plasmid

#23050

Depositor

Jon Ashwell

Publication

Zhu et al Curr Biol. 2007 Aug 21. 17(16):1438-43.

Insert

Optineurin (OPTN Human)

Tags

His

Expression

Mammalian

Mutation

N/A

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pSpCas9_T2A_mCherry sg1-sg2

Plasmid

#192479

Purpose

All-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1

Depositor

Michael Yaffe

Publication

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

SpCas9

Tags

T2A mCherry

Expression

Mammalian

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-EF1a-dCas9-HA-BFP-KRAB-NLS

Plasmid

#102244

Purpose

Lentiviral expression of dCas9-HA-BFP-KRAB-NLS for CRISPRi driven by a hEF1alpha promoter

Depositor

Jacob Corn

Insert

dCas9-HA-BFP-KRAB-NLS

Use

CRISPR and Lentiviral

Tags

HA-TagBFP-KRAB-NLS

Expression

Mammalian

Promoter

EF1a

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pcDNA3.2 TDP-43 5F-L YFP

Plasmid

#84914

Purpose

Mammalian expresion of TDP-43 5F-L YFP

Depositor

Aaron Gitler

Publication

Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

Insert

TDP-43 (TARDBP Human)

Tags

YFP

Expression

Mammalian

Mutation

F147L, F149L, F194L, F229L, F231L

Promoter

CMV

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pDR-GW AT1.03R122K/R126K

Plasmid

#28005

Depositor

Wolf Frommer

Publication

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

AT1.03R122K/R126K

Tags

mVenus and mseCFP

Expression

Yeast

Mutation

R122K, R126K

Available since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pLBH545_Tet-Cas14a1_Locus

Plasmid

#112501

Purpose

Expresses Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

Depositor

Jennifer Doudna

Publication

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14a1

Use

CRISPR

Expression

Bacterial

Promoter

Tet

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA

Plasmid

#85756

Purpose

All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).

Depositor

Lawrence Stanton

Publication

Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

Inserts

Csy4-T2A-SpRFN-P2A-GFP
gRNA

Use

CRISPR

Tags

Csy4, Csy4 recognition sequence, FokI fusion via …

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CMV and U6

Available since

Jan. 20, 2017

Availability

Academic Institutions and Nonprofits only

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RfxCas13d-backbone

Plasmid

#165078

Purpose

Expression of RfxCas13d sgRNAs from a U6 promoter

Depositor

Ling-Ling Chen

Publication

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

Scaffold gRNA

Expression

Mammalian

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEN_TTmiRc2

Plasmid

#25752

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE-tight promoter.

Depositor

Iain Fraser

Publication

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Gateway Cloning

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pKM596

Plasmid

#8837

Depositor

David Waugh

Publication

Fox et al FEBS Lett. 2003 Feb 27. 537(1-3):53-7.

Type

Empty backbone

Tags

E. coli MBP

Expression

Bacterial

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pUCmini-iCAP-PHP.N

Plasmid

#127851

Purpose

non-standard AAV2 rep-AAV-PHP.N cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Publication

Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

Insert

Synthetic construct isolate AAV-PHP.N VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti-X1-hygro-mCherry-RAMP4

Plasmid

#118391

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Publication

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

RAMP4 (SERP1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

EIF-1a promoter

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCMV5 MEKK3 WT

Plasmid

#12186

Depositor

Gary Johnson

Publication

Blank et al J Biol Chem. 1996 Mar 8. 271(10):5361-8.

Insert

MEKK3 (Map3k3 Mouse)

Tags

HA

Expression

Mammalian

Available since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pDsRed Monomer C1-YAP2-S127A mutant

Plasmid

#19058

Depositor

Marius Sudol

Publication

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

Red-FPP

Expression

Mammalian

Mutation

Serine 127 changed to Alanine

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pLGB31

Plasmid

#126621

Purpose

Suicide vector for allelic replacement in Bacteroides, inulin selection and rhamnose-inducible ss-Bfe1 counterselection

Depositor

Laurie Comstock

Publication

Garcia-Bayona et al mBio. 2019 Aug 13;10(4). pii: mBio.01762

Type

Empty backbone

Use

Allellic exchange in bacteria

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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HA-14-3-3 eta

Plasmid

#116887

Purpose

Expresses HA-tagged human 14-3-3 eta in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Publication

Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

Insert

14-3-3 eta (YWHAH Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP

Plasmid

#188899

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

Depositor

Marco Jost, Jonathan Weissman

Publication

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS, P2A-GFP, and Zim3 KRAB-NLS fusion

Promoter

SFFV

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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