We narrowed to 2,819 results for: aen

1. pAc5.1B-lambdaN-HA-CeAIN2_254-400-DmGW182_862-1384_R

   Plasmid

   #147406

   Purpose

   Insect Expression of CeAIN2_254-400-DmGW182_862-1384

   Depositor

   Elisa Izaurralde

   Article

   Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

   Insert

   CeAIN2_254-400-DmGW182_862-1384 (gw Nematode, Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Deletion o f2 AA (SD) at position 378-379 compare…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384_R

   Plasmid

   #147384

   Purpose

   Insect Expression of CeAIN2_254-400-DmGW182_635-1384

   Depositor

   Elisa Izaurralde

   Article

   Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

   Insert

   CeAIN2_254-400-DmGW182_635-1384 (gw Nematode, Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Deletion of 2 AA (SD) at position 378-379 compare…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pX459_V2.0_pSpCas9(BB)-2A-Puro_T-REX17_Ex1_KI

   Plasmid

   #195504

   Purpose

   Cas9-2A-Puro expression vector bearing a sgRNA targeting Exon 1 of human lncRNA T-REX17

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   sgRNA
   

   Use

   CRISPR

   Tags

   3XFLAG, Puro, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUW-crRNA-1-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP

   Plasmid

   #194889

   Purpose

   Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and crRNA-1 targeting the human MECP2 locus.

   Depositor

   Shawn Liu

   Article

   Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

   Inserts

   dLbCpf1-NLS-hCTCF_WT
   U6-crRNA1
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Human codon optimized catalytically inactive LbCp…

   Promoter

   TRE and U6

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. FUW-crRNA-1-U6_TRE-dLbCpf1-NLS-hCTCF_Q418A-HA-P2A-tagBFP

   Plasmid

   #194891

   Purpose

   Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-Q418A mutant and crRNA-1 targeting the human MECP2 locus.

   Depositor

   Shawn Liu

   Article

   Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

   Inserts

   dLbCpf1-NLS-hCTCF_Q418A
   U6-crRNA1
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Human codon optimized catalytically inactive LbCp…

   Promoter

   TRE and U6

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FUW-crRNA-2-U6_TRE-dLbCpf1-NLS-hCTCF_R396A-HA-P2A-tagBFP

   Plasmid

   #194896

   Purpose

   Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-R396A mutant and crRNA-2 targeting the human MECP2 locus.

   Depositor

   Shawn Liu

   Article

   Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

   Inserts

   dLbCpf1-NLS-hCTCF_R396A
   U6-crRNA2
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Human codon optimized catalytically inactive LbCp…

   Promoter

   TRE and U6

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FUW-crRNA1and2-U6_TRE-dLbCpf1-NLS-hCTCF_R396A-HA-P2A-tagBFP

   Plasmid

   #194888

   Purpose

   Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-R396A mutant and crRNA-1 and -2 targeting the human MECP2 locus.

   Depositor

   Shawn Liu

   Article

   Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

   Inserts

   dLbCpf1-NLS-hCTCF_R396A
   U6-crRNA1and2
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Human codon optimized catalytically inactive LbCp…

   Promoter

   TRE and U6

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FUW-crRNA1and2-U6_TRE-dLbCpf1-NLS-hCTCF_Q418A-HA-P2A-tagBFP

   Plasmid

   #194887

   Purpose

   Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-Q418A mutant and crRNA-1 and -2 targeting the human MECP2 locus.

   Depositor

   Shawn Liu

   Article

   Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

   Inserts

   dLbCpf1-NLS-hCTCF_Q418A
   U6-crRNA1and2
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Human codon optimized catalytically inactive LbCp…

   Promoter

   TRE and U6

   Available since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FUW-crRNA-2-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP

   Plasmid

   #194893

   Purpose

   Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and crRNA-2 targeting the human MECP2 locus.

   Depositor

   Shawn Liu

   Article

   Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

   Inserts

   dLbCpf1-NLS-hCTCF_WT
   U6-crRNA2
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Human codon optimized catalytically inactive LbCp…

   Promoter

   TRE and U6

   Available since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pv9_MBD1_MBD-R22A-13XL-BASU

    Plasmid

    #179417

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1.MBD-R22A-13XL-BASU

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    MBD1.MBD-R22A-13XL-BASU
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGST2-GST-TEV-OPTN S177D S473D

    Plasmid

    #187827

    Purpose

    Plasmid for the expression and purification of GST-TEV-OPTN S177D S473D. Internal Ref: SMC1588

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    OPTN (OPTN Human)
    

    Use

    Tags

    GST-TEV

    Expression

    Bacterial

    Mutation

    S177D S473D

    Promoter

    Available since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pv6_CBX7_Chromo_TAF3_Phd-DW890/891AA

    Plasmid

    #179402

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo - TAF3_Phd(DW890/891AA)

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX7_Chromo - TAF3_Phd(DW890/891AA)
    

    Use

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    Phd domain DW890/891AA

    Promoter

    CAGGS

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389A_R

    Plasmid

    #147394

    Purpose

    Insect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389A

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389A (TNRC6C Nematode, Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Deletion of 2 AA (SD) at position 378-379 compare…

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pWZ157

    Plasmid

    #163639

    Purpose

    pcn-1>PCN-1::GFP expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I

    Depositor

    David Matus

    Article

    Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

    Insert

    pcn-1 (pcn-1 Nematode)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    pcn-1

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTD73

    Plasmid

    #172897

    Purpose

    Intestinal promoter ges-1P::TIR1::F2A::TagBFP2::AID* with homology arms for insertion in ttTi4348 site on chromosome I

    Depositor

    David Reiner

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    TIR-1 (TIR1 Mustard Weed)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTD81

    Plasmid

    #172902

    Purpose

    Hypodermal promoter col-10P::TIR1::F2A::TagBFP2::AID* with homology arms for insertion in ttTi5605 site on chromosome II

    Depositor

    David Reiner

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    TIR-1 (TIR1 Mustard Weed)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSMT3-FBF2 SS/Y

    Plasmid

    #166973

    Purpose

    Expresses FBF-2 with SS/Y mutations in repeat 5 in E. coli for purification and crystallization studies

    Depositor

    Traci Hall

    Article

    Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

    Insert

    FBF-2 (fbf-2 Nematode)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    FBF-2 PUM RNA binding domain; amino acids 164-575…

    Promoter

    T7

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSMT3-FBF2 AS/Y

    Plasmid

    #166974

    Purpose

    Expresses FBF-2 with AS/Y mutations in repeat 5 in E. coli for purification and crystallization studies

    Depositor

    Traci Hall

    Article

    Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

    Insert

    FBF-2 (fbf-2 Nematode)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    FBF-2 PUM RNA binding domain; amino acids 164-575…

    Promoter

    T7

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDEST40-2XHA-Src-S4-UD-mCherr

    Plasmid

    #140307

    Purpose

    Expresses Flag-tagged human Src-S4-UD-mCherry in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    contains only the SH4 and UD regions fused to mCh…

    Promoter

    CMV

    Available since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDEST40-2XFL-Src-S32

    Plasmid

    #140309

    Purpose

    Expresses Flag-tagged human Src-S32 in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    contains only the SH3 and SH2 domains

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge