We narrowed to 2,968 results for: GRI;

1. pKLV2-EF1a-BsdCas9-W

   Plasmid

   #67978

   Purpose

   Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminus

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   EF1a-Cas9 cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Cas9 fused with Flag-tag and a NLS at the N termi…

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AP1muA-mStayGold-polyA-Hygro HR

   Plasmid

   #229679

   Purpose

   Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with monomeric StayGold. Contains a hygromycin resistance cassette for selection of edited cells

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

   Insert

   genomic sequence AP1M1 locus (AP1M1 Human)
   

   Use

   CRISPR

   Tags

   monomeric StayGold

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFastBac Dual wt p110α/p85α

   Plasmid

   #199302

   Purpose

   co-expression of His-tagged p110α wt and Avi-tagged p85α wt proteins

   Depositor

   Peter Vogt

   Article

   Favalli et al Nat Chem. 2021 Jul;13(7):714. doi: 10.10

   Insert

   His-tagged p110α wt and Avi-tagged p85α wt (PIK3CA, PIK3R1 Human)
   

   Use

   Tags

   Avi and His

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET21a(+)-HIV RT-6xHis-RBS-prot

   Plasmid

   #159149

   Purpose

   expresses His-tagged HIV reverse transcriptase and untagged HIV protease

   Depositor

   Andrea Pauli

   Article

   Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d

   Inserts

   Human immunodeficiency virus 1 (HIV-1) reverse transcriptase
   Human immunodeficiency virus 1 (HIV-1) protease
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   HIV-1 group M/subtype B – BH10 strain

   Promoter

   Available since

   Sept. 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmAG-W

   Plasmid

   #67976

   Purpose

   CRISPR gRNA expression vector with an improved scaffold and puro/mAG markers

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKpuro2AmAG cassette, WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   human U6 promoter, mouse PGK promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AP1muA-Halo-ALFA-polyA-G418 HR

   Plasmid

   #229681

   Purpose

   Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells.

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

   Insert

   genomic sequence AP1M1 locus (AP1M1 Human)
   

   Use

   CRISPR

   Tags

   Halo, ALFA

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AP1muA-SNAP-V5-polyA-Puro HR

   Plasmid

   #229680

   Purpose

   Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with SNAP tag and a V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

   Insert

   genomic sequence AP1M1 locus (AP1M1 Human)
   

   Use

   CRISPR

   Tags

   SNAP, V5

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-CAG-FLEX-SomaGCaMP7

   Plasmid

   #182942

   Purpose

   Cre-dependent expression of cell body-targeted GCaMP7f, under CAG promoter. As bright as conventional GCaMP7f.

   Depositor

   Edward Boyden

   Article

   Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

   Insert

   Soma-GCaMP7
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. COVID-SARS2 NSP14/10

   Plasmid

   #159613

   Purpose

   Bacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10

   Depositor

   Opher Gileadi

   Article

   Baddock et al Nucleic Acids Res. 2022 Jan 17. pii: 650

   Inserts

   Covid-SARS2 Nsp14 (ORF1ab Synthetic, COVID-SARS2)
   Covid-SARS2 Nsp10
   

   Use

   Tags

   His6, TEV cleavage site and none

   Expression

   Bacterial

   Mutation

   Codon-optimized for E. coli expression

   Promoter

   (Bicistronic) and T7 - LacO

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-W

    Plasmid

    #67981

    Purpose

    Cas9 activity reporter (control) with mCherry and GFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKLV2-U6gRNA3(BbsI)-PGKpuro2ABFP

    Plasmid

    #67990

    Purpose

    CRISPR gRNA expression vector with the conventional scaffold and puro/BFP markers without WPRE

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette with the conventional scaffold, PGKpuro2ABFP cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pT3-EF1A-CCNE2

    Plasmid

    #162554

    Purpose

    Overexpression of CCNE2

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    Overexpression of CCNE2 (CCNE2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF1A

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-EF1a-FLEX-SomaGCaMP7

    Plasmid

    #182941

    Purpose

    Cre-dependent expression of cell body-targeted GCaMP7f, under EF1a promoter. As bright as conventional GCaMP7f.

    Depositor

    Edward Boyden

    Article

    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

    Insert

    Soma-GCaMP7
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. elavl3:SomaGCaMP7f

    Plasmid

    #158760

    Purpose

    Cell body-targeted GCaMP7f, under HuC promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.

    Depositor

    Edward Boyden

    Article

    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

    Insert

    SomaGCaMP7
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    HuC promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. px330-Axin1

    Plasmid

    #162543

    Purpose

    sgRNA targeting Axin1

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Axin1 (Axin1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKLV2-U6gRNA5(gBFP)-PGKGFP2ABFP-W

    Plasmid

    #67984

    Purpose

    Cas9 activity reporter with GFP and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Inserts

    U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
    Guide RNA targeting modified BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAc-mCh-Tub

    Plasmid

    #24288

    Depositor

    Ron Vale

    Article

    Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

    Insert

    Alpha-Tubulin (alphaTub84B Fly)
    

    Use

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pCAGGS-ACE2-his expression plasmid

    Plasmid

    #158089

    Purpose

    Expresses ACE2 in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    ACE2 (ACE2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP

    Plasmid

    #67991

    Purpose

    CRISPR gRNA expression vector with an improved l scaffold and puro/BFP markers without WPRE

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette with an improved scaffold, PGKpuro2ABFP cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-Syn-Soma-jGCaMP8f

    Plasmid

    #182943

    Purpose

    Cell body-targeted jGCaMP8f, under synapsin promoter.

    Depositor

    Edward Boyden

    Article

    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

    Insert

    Soma-jGCaMP8f
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge