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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,006 results for: actin

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Showing: 2641 - 2660 of 6006 results

  1. pExpreS2-1-C3-10H-SIII

    Plasmid
    #175444
    Purpose
    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal 10His-TwinStrep tagged proteins with ExpreS2 Platform
    Depositor
    Wyatt Yue
    Publication
    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8
    Type
    Empty backbone
    Tags
    3C-10His-TwinStrep and BiP Secretion Peptide
    Expression
    Insect
    Promoter
    Fused Actin-HSP70
    Available since
    March 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    175444-plasmid-map-sequence-id-358135

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  3. pExpreS2-1-C3-PA-10H-SIII

    Plasmid
    #175443
    Purpose
    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform
    Depositor
    Wyatt Yue
    Publication
    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8
    Type
    Empty backbone
    Tags
    3C-Protein A-10His-TwinStrep and BiP Secretion Pe…
    Expression
    Insect
    Promoter
    Fused Actin-HSP70
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    175443-plasmid-map-sequence-id-360135

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  4. pAAV-CAG-GFP (AAV6)

    Viral prep
    #37825-AAV6
    Purpose
    Ready-to-use AAV6 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    GFP
    Available since
    July 16, 2025
    Availability
    Academic Institutions and Nonprofits only

  5. pAAV-CAG-FlpO (AAV Retrograde)

    Viral prep
    #183412-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-CAG-FlpO (#183412). In addition to the viral particles, you will also receive purified pAAV-CAG-FlpO plasmid DNA. CAG-driven expression of the recombinase FlpO. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Janelia Viral Tools
    Promoter
    CAG
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only

  6. pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid
    #216246
    Purpose
    Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration
    Depositor
    Elina Ikonen
    Publication
    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9 and sgAAVS1.5
    Use
    CRISPR; Human safe harbor locus (a avs1) site-spe…
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216246-plasmid-map-sequence-id-426842

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  7. pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST (AAV9)

    Viral prep
    #109048-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST (#109048). In addition to the viral particles, you will also receive purified pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST plasmid DNA. Cre-dependent expression of the anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites, translated from an internal ribosomal entry site (IRES) following mRuby3 expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hillel Adesnik
    Publication
    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/
    Promoter
    CAG
    Tags
    mRuby3
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only

  8. pCAG-CBE4max-SpRYc-P2A-EGFP

    Plasmid
    #208336
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYc
    Depositor
    Pranam Chatterjee
    Publication
    Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    Sc++ (1-1119), SpRY (1111-1368), D10A
    Promoter
    CAG
    Available since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    208336-plasmid-map-sequence-id-439176

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  10. PB-PE

    Plasmid
    #162796
    Purpose
    All-in-one prime editor piggyBac transposon
    Depositor
    Tobias Cantz
    Publication
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9_ H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    162796-plasmid-map-sequence-id-320034

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  12. Tol2-CAG::Cytbow

    Plasmid
    #158991
    Purpose
    Multicolor cytoplasmic cell labeling with a genome-integrating Tol2 transposon vector (randomly expressing 3 tdTomato, mCerulean or mEYFP upon Cre recombination)
    Depositor
    Jean Livet
    Publication
    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10
    Insert
    Cytbow
    Tags
    The default expressed EBFP2 is fused to H2B
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    158991-plasmid-map-sequence-id-311790

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  13. LiOn-CAG∞IRFP

    Plasmid
    #154017
    Purpose
    Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein IRFP670 from a CAG promoter upon action of the piggyBac transposase
    Depositor
    Jean Livet
    Publication
    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d
    Insert
    IRFP670
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    154017-plasmid-map-sequence-id-308109

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  15. PB-CAG-DDdCas9VP192-T2A-GFP-IRES-Neo

    Plasmid
    #102885
    Purpose
    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Publication
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DDdCas9VP192-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    102885-plasmid-map-sequence-id-259794

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  18. pCAGGS-PHFF

    Plasmid
    #100280
    Purpose
    Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.
    Depositor
    Kazuhiro Aoki
    Publication
    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p
    Insert
    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    Tags
    FLAG, T7 and Mitochondria-targeting sequence (MTS…
    Expression
    Mammalian
    Mutation
    All genes are codon-optimized for expression in h…
    Promoter
    CAG promoter
    Available since
    Sept. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    100280-plasmid-map-sequence-id-194335

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Showing: 2641 - 2660 of 6006 results