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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,310 results for: eng

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Showing: 2641 - 2660 of 14310 results

  4. dCas9-CBP FL

    Plasmid
    #179550
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of full length human CBP driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Publication
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of full length human CBP (CREBBP Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179550-plasmid-map-sequence-id-354775

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  8. pDN80

    Plasmid
    #61349
    Purpose
    LINuS-flex: modular LINuS construct enabling introduction of any protein encoding sequence, NES and NLS. The sequence of interest replaces the LexA DBD-MBP-VP64 fragment.
    Depositor
    Barbara Di Ventura, Roland Eils
    Publication
    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.
    Type
    Empty backbone
    Tags
    AsLOV2 wt and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    61349-plasmid-map-sequence-id-99714

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  12. pAR18_StrepI_mFASm_H8_pET22b

    Plasmid
    #122847
    Purpose
    Expresses murine type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.
    Depositor
    Martin Grininger
    Publication
    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi
    Insert
    mouse FASN/ mouse fatty acid synthase (Fasn Mouse)
    Tags
    His8 tag and Twin-Strep tag
    Expression
    Bacterial
    Mutation
    wildtype
    Promoter
    T7 promoter
    Available since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    122847-plasmid-map-sequence-id-238541

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  18. AAV pCAG-FLEX-mRuby3-WPRE

    Plasmid
    #99279
    Purpose
    Can be used to generate AAV virus that will express mRuby3 in the presence of Cre
    Depositor
    Rylan Larsen
    Insert
    mRuby3
    Use
    AAV and Cre/Lox
    Available since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    99279-plasmid-map-sequence-id-181812

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  19. pAC-85b

    Plasmid
    #53282
    Purpose
    Contains crtE, crtI, crtY genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and will produce beta-carotene in E. coli when complemented with a gene encoding phytoene synthase
    Depositor
    Francis X Cunningham Jr
    Publication
    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E
    Insert
    crtE, crtY, crtI
    Use
    Low copy number bacterial cloning vector
    Promoter
    endogenous promoters
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    53282-plasmid-map-sequence-id-82995

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  20. pBbB8k-csg-NbGFP

    Plasmid
    #166858
    Purpose
    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a GFP-specific nanobody via a flexible linker. Derived from https://www.addgene.org/35363/.
    Depositor
    Neel Joshi
    Publication
    Birnbaum et al Adv Sci (Weinh). 2021 May 3;8(11):200469
    Insert
    csgBACEFG, with GFP-specific nanobody fused to CsgA via flexible linker
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166858-plasmid-map-sequence-id-329027

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Showing: 2641 - 2660 of 14310 results