We narrowed to 6,531 results for: poly

1. pGBW-m4046241

   Plasmid

   #148997

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;MBP

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pNIC28-BpWcbI

   Plasmid

   #125591

   Purpose

   Overexpression plasmid for production of WcbI from Burkholderia pseudomallei

   Depositor

   Nicholas Harmer

   Article

   Vivoli et al IUCrJ. 2013 Oct 18;1(Pt 1):28-38. doi: 1

   Insert

   BpWcbI (wcbI Burkholderia pseudomallei)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pIAGO-Hap2

   Plasmid

   #114460

   Purpose

   Expression of hybrid AmphDI-PerDI (Hap) glycosyltansferase and perosamine synthase in streptomycetes

   Depositor

   Patrick Caffrey

   Article

   Hutchinson et al Chem Biol. 2010 Feb 26;17(2):174-82. doi

   Insert

   Hap2 + perDII
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ermE

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTOPO-rDNA-NTS

   Plasmid

   #23992

   Depositor

   Susan Baserga

   Article

   Gallagher et al Genes Dev. 2004 Oct 15. 18(20):2506-17.

   Insert

   rDNA NTS
   

   Use

   Bacterial cloning - topo

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 11, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pGMEH-P38-luxABct6

   Plasmid

   #27063

   Depositor

   Dirk Schnappinger

   Article

   Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

   Insert

   P#38-luxAB-DAS+4
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SF-1 (antisense)

   Plasmid

   #28181

   Depositor

   Bernard Schimmer

   Article

   Frigeri et al Endocrinology. 2002 Oct . 143(10):4031-7

   Insert

   SF-1 (Nr5a1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Reverse Orientation

   Promoter

   Available since

   May 12, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pG6(3'Pro) (P#1478)

   Plasmid

   #14660

   Depositor

   Matija Peterlin

   Article

   Taube et al Mol Cell Biol. 2002 Jan . 22(1):321-31.

   Insert

   UAS repeats
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pCellFree_G03 PC

   Plasmid

   #67132

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   PC (PC Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pJB75

   Plasmid

   #226320

   Purpose

   E. coli protein expression for protein purification

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   6xHis-(Y to A MR1-6)-strep
   

   Use

   Tags

   6xHis and Strep

   Expression

   Bacterial

   Mutation

   N-terminal domain deleted, C-terminal domain dele…

   Promoter

   T7

   Available since

   Oct. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFH77

    Plasmid

    #125816

    Purpose

    Level0 enCas9-PolI3M-TBD, wheat codon optimized

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    enCas9-PolI3M-TBD, wheat codon optimized
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pScPPX1

    Plasmid

    #233713

    Purpose

    Bacterial expression of the yeast PPX1 gene

    Depositor

    Michael Downey

    Article

    Baijal et al bioRxiv 2025.02.12.637445

    Insert

    ScPpx1 (PPX1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 7, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pGEM4Z-Fluc

    Plasmid

    #183474

    Purpose

    This plasmid can be used to prepare Fluc mRNA by In Vitro Transcription

    Depositor

    Chantal Pichon

    Article

    Perche et al Mol Ther Nucleic Acids. 2019 Sep 6;17:76

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEasy Flox

    Plasmid

    #11725

    Depositor

    Klaus Rajewsky

    Article

    Schenten et al Eur J Immunol. 2002 Nov . 32(11):3152-60

    Insert

    pEasyFlox
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FLAG-Pol-IIΔ

    Plasmid

    #35176

    Depositor

    Benjamin Blencowe

    Article

    Rosonina et al RNA. 2004 Apr;10(4):581-9.

    Insert

    RPBI (POLR2A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Deletion of the entire C-terminal domain (repeats…

    Promoter

    CMV

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FLAG-Pol-II 1-22

    Plasmid

    #35178

    Depositor

    Benjamin Blencowe

    Article

    Rosonina et al RNA. 2004 Apr;10(4):581-9.

    Insert

    RPBI (POLR2A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Deletion of C-terminal domain repeats 23-52

    Promoter

    CMV

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

16. FLAG-Pol-II 31-52

    Plasmid

    #35179

    Depositor

    Benjamin Blencowe

    Article

    Rosonina et al RNA. 2004 Apr;10(4):581-9.

    Insert

    RPBI (POLR2A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Deletion of C-terminal domain repeats 1-30

    Promoter

    CMV

    Available since

    Oct. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pACYCDuet-1_Ec_rpoZ

    Plasmid

    #128837

    Purpose

    E. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.

    Depositor

    Seth Darst

    Article

    Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

    Insert

    rpoZ (rpoZ E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGS-21a his6-GST-WTPhi29DNAP

    Plasmid

    #166863

    Purpose

    To produce a dual affinity-tagged Phi29 DNA polymerase

    Depositor

    Paul Blainey, Stephen Quake

    Article

    Blainey et al Nucleic Acids Res. 2011 Mar;39(4):e19. d

    Insert

    Phi29 Polymerase codon-optimized for overexpression in E. coli
    

    Use

    Tags

    GST and his6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBTK522-FAST-PETase

    Plasmid

    #214754

    Purpose

    plasmid for expression FAST-PETase in Pseudomonas putida

    Depositor

    Hal Alper

    Article

    Lu et al Nature. 2022 Apr;604(7907):662-667. doi:

    Insert

    FAST-PETase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Ube2r1

    Plasmid

    #31426

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Ubiquitin Conjugating Enzyme (CDC34 Human)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only