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Plasmid#68940Purposetetracycline-inducible expression vector for Staphylococcus aureusDepositorTypeEmpty backboneUseTet inducible e. coli/staphylococcus shuttle vect…TagsExpressionBacterialMutationPromoterAvailable sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only
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pHL32 (in cHNL135)
Plasmid#24387DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJK122
Plasmid#74295PurposeProduces Acetobacter aceti 1023 hypothetical acetic acid resistance protein B, cleavable N-terminal NusA fusionDepositorInserthypothetical acetic acid resistance protein B
UseTagsNusAExpressionBacterialMutationPromoterT7Available sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK103
Plasmid#73265PurposeProduces Acetobacter aceti 1023 hypothetical acetic acid resistance protein B, C-terminal His6 tag (AaAarBH6)DepositorInserthypothetical acetic acid resistance protein B
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD-mTagBFP2
Plasmid#34632DepositorInsertmTagBFP2
UseTagsExpressionBacterialMutationI174A compared to mTagBFP (enhanced photostabilit…PromoterAvailable sinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pIMAY*
Plasmid#121441PurposeAllelic exchange plasmid with PheS* counter-selection for Staphylococcus aureusDepositorTypeEmpty backboneUseEscherichia coli/staphylococcus aureus shuttle ve…TagsExpressionMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIRES-mCerulean-FRB-Cdc42/N12-Cdc42/13C(Q61L)-FKBP-mVenus-CAAX
Plasmid#214278PurposeExpress split-Cdc42 fragments that are fused with CIDs and FPsDepositorInsertsUseTagsmCerulean (on insert 1) and mVenus (on insert 2)ExpressionMammalianMutationCdc42-Q61LPromoterCMVAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES-mCerulean-FRB-Cdc42/N12-Cdc42/13C(T17N)-FKBP-mVenus-CAAX
Plasmid#214279PurposeExpress split-Cdc42 fragments that are fused with CIDs and FPsDepositorInsertsUseTagsmCerulean (on insert 1) and mVenus (on insert 2)ExpressionMammalianMutationCdc42-T17NPromoterCMVAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES-mCerulean-FRB-RhoA/N12-RhoA/13C(Q63L)-FKBP-mVenus-CAAX
Plasmid#214282PurposeExpress split-RhoA fragments that are fused with CIDs and FPsDepositorInsertsUseTagsmCerulean (on insert 1) and mVenus (on insert 2)ExpressionMammalianMutationRhoA-Q63LPromoterCMVAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES-mCerulean-FRB-RhoA/N12-RhoA/13C(T19N)-FKBP-mVenus-CAAX
Plasmid#214283PurposeExpress split-RhoA fragments that are fused with CIDs and FPsDepositorInsertsUseTagsmCerulean (N terminal on insert 1) and mVenus (on…ExpressionMammalianMutationRhoA-T19NPromoterCMVAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FDD
Plasmid#80629PurposeFluorescent destablizing domain for regulating protein stability and fluorescenceDepositorInsertUnaG
UseRetroviralTagsHA-mcherry-linkerExpressionMutationmutations A36V, R136GPromoterLTRAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMi(3xP3-DsRed)
Plasmid#102539PurposeTransgenesis vector based on the Minos transposon, carrying the 3xP3-DsRed markerDepositorInsertMinos transpoable element with 3xP3-DsRed marker
UseTagsExpressionMutationPromoterAvailable sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS6-mPSD95a-TSa-HA
Plasmid#42235PurposeTimeSTAMP method for tagging and visualization of newly synthesized proteins. TimeSTAMPa cassette used for for low abundance proteins or proteins with slow ongoing synthesis ratesDepositorInsertPSD95
UseTagsTSa-HAExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS6-mPSD95a-TSt-HA
Plasmid#42237PurposeTimeSTAMP method for tagging and visualization of newly synthesized proteins. TimeSTAMPt cassette used for highly expressed proteins or proteins with faster ongoing synthesis ratesDepositorInsertPSD95
UseTagsTSt-HAExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
SAPDF-pET21a(+)
Plasmid#12649DepositorInsertSA Peptide deformylase
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMV762-Peredox-mCherry
Plasmid#90217PurposeFluorescent reporter for NADH:NAD+ ratios in Mycobacteria.DepositorInsertCodon Optimized Peredox-mCherry
UseShuttle vectorTagsExpressionBacterialMutationPromotergroELAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_60
Plasmid#109254PurposeTALEsp2 stabilized promoter driving GFPDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterTALEsp2 stabilized promoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_59
Plasmid#109253PurposeTALEsp1 stabilized promoter driving GFPDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterTALEsp1 stabilized promoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
MTST5
Plasmid#86602PurposeCustom designed synthetic DNA fragment lacking homology to natural sequences for preparation of internal standards for RNA-seq in metatranscriptomics.DepositorInsertMTST5
UseInternal standard reverse transcriptionTagsExpressionMutationPromoterT7Available sinceSept. 15, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pT7_circRAJ31
Plasmid#69931PurposeExpresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. Under control of a T7 promoter.DepositorInsertpT7_circRAJ31
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only