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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 50,354 results for: cmv promoter

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Showing: 2661 - 2680 of 50354 results

  2. pDAC439

    Plasmid
    #195237
    Purpose
    Mammalian expression of Csm complex from single promoter; RFP backbone (Used for RNA knockdown)
    Depositor
    Jennifer Doudna
    Publication
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FLAG-NLS-Csm5-2A-FLAG-NLS-Csm4-2A-FLAG-NLS-Csm3-2A-FLAG-NLS-Csm2; RFP-2A-FLAG-NLS-Cas6-2A-FLAG-NLS-Csm1; crRNA
    Expression
    Mammalian
    Available since
    Jan. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195237-plasmid-map-sequence-id-382945

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  3. Cx43-msfGFP

    Plasmid
    #69007
    Purpose
    Expresses rat Cx43 (Gja1 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.
    Depositor
    David Spray
    Publication
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gja1 (Gja1 Rat)
    Tags
    monomerized superfolder GFP
    Expression
    Mammalian
    Promoter
    cmv
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    69007-plasmid-map-sequence-id-121423

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  4. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213027
    Purpose
    Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Publication
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    D702Y (RT- reverse transcriptase catalytic dead)
    Promoter
    CMV
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    213027-plasmid-map-sequence-id-452358

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  5. pcDNA-emerin-EGFP-polyA

    Plasmid
    #59747
    Purpose
    Express emerin-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.
    Depositor
    Yi Zhang
    Publication
    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    emerin-EGFP
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    59747-plasmid-map-sequence-id-94051

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  6. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213029
    Purpose
    Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Publication
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213029-plasmid-map-sequence-id-422050

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  7. pDisplay sHRPa-KDEL

    Plasmid
    #73149
    Purpose
    Large fragment of split HRP targeted for expression in the lumen of the endoplasmic reticulum (ER). CMV promoter.
    Depositor
    Alice Ting
    Publication
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sHRPa-KDEL
    Tags
    FLAG tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73149-plasmid-map-sequence-id-134530

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  10. pEGFP-C1 Utr261-EGFP

    Plasmid
    #58471
    Purpose
    Expresses a cytoplasmic actin filament reporter, the tandem CH domains (a.a. 1-261) from human utrophin, on a CMV promoter
    Depositor
    Dyche Mullins
    Publication
    Belin et al Bioarchitecture. 2015 Aug 28:1-14.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Utr261 (UTRN Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58471-plasmid-map-sequence-id-90380

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  14. ShuttleNSE

    Plasmid
    #50958
    Purpose
    A backbone for adenovirus creation by the AdEasy method, similar to pShuttle-CMV (Addgene #16403), except it uses a Neuron-specific enolase (NSE) promoter instead of the CMV promoter
    Depositor
    James Bamburg
    Publication
    Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:
    Type
    Empty backbone
    Use
    Adenoviral
    Expression
    Mammalian
    Promoter
    NSE
    Available since
    Feb. 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    50958-plasmid-map-sequence-id-76714

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  16. Human b2 nicotinic subunit crystallization construct

    Plasmid
    #85842
    Purpose
    pEZT-BM vector with beta2 gene after CMV promoter, published crystallization construct
    Depositor
    Ryan Hibbs
    Publication
    Morales-Perez et al Nature. 2016 Oct 3;538(7625):411-415. do
    Insert
    Human b2 nAChR subunit crystallization construct (CHRNB2 Human)
    Use
    Baculovirus (bacmam) production and mammalian exp…
    Tags
    C-terminal StrepII tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    85842-plasmid-map-sequence-id-171117

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  18. pDisplay sHRPb-KDEL

    Plasmid
    #73150
    Purpose
    Small fragment of split HRP targeted for expression in the lumen of the endoplasmic reticulum (ER). CMV promoter.
    Depositor
    Alice Ting
    Publication
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sHRPb-KDEL
    Tags
    V5 tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73150-plasmid-map-sequence-id-134536

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  19. msfGFP-Cx43

    Plasmid
    #69024
    Purpose
    Expresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to msfGFP (FP fused to NT of connexin). CMV promoter.
    Depositor
    David Spray
    Publication
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cx43 (Gja1 Rat)
    Tags
    sfGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    69024-plasmid-map-sequence-id-127982

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Showing: 2661 - 2680 of 50354 results