We narrowed to 5,835 results for: pcas

1. pCAG CAG Glucagon-ICAM1-3xHA-pre-mGRASP

   Plasmid

   #153008

   Purpose

   Expresses glucagon peptide on mammalian cell surface

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   Glucagon-ICAM1-pre-mGRASP
   

   Tags

   3x HA

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-dSaCas9-2xNLS

   Plasmid

   #107319

   Purpose

   Expresses SpCas9 fused to dSaCas9 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SpCas9-SaCas9 fusion
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   SpCas9 is fused to nuclease dead SaCas9 (D10A, N5…

   Promoter

   CMV IE94

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-DIO-inv[roxSTOProx-EB3 mNeonGreen-bGH]

   Plasmid

   #196885

   Purpose

   Dual Cre/Dre-dependent expression of EB3-mNeon. Used as functional (labelling of microtubule plus ends) Cre-Dre cotransfection reporter

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   roxSTOProx-EB3 mNeonGreen-bGH (MAPRE3 Human)
   

   Use

   Cre/Lox; Dre/rox

   Expression

   Mammalian

   Promoter

   Quimeric CAG

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCSD_2XNLS-SpCas9WT-NLS-3XHA-2XNLS-NmdCas9-NLS

   Plasmid

   #107327

   Purpose

   Expresses SpCas9 fused to dNmCas9 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SpCas9-NmCas9 fusion
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   SpCas9 is fused to nuclease dead NmCas9 (D16A, D5…

   Promoter

   CMV IE94

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCSD_2xNLS-SpCas9MT3-NLS-3xHA-NLS-dSaCas9-2xNLS

   Plasmid

   #107318

   Purpose

   Expresses SpCas9 (R1335K) fused to dSaCas9 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SpCas9-SaCas9 fusion
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   SpCas9 (R1335K) fused to nuclease dead SaCas9 (D1…

   Promoter

   CMV IE94

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBW2602_pCAG-PV1-iCre-N256-L1-FRB-NLS-BGHpA

   Plasmid

   #108735

   Purpose

   Expresses N-terminus to aa256 of Cre recombinase fused to FRB CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Cre recombinase fragment N-terminus to aa256
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   FRB,NLS

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBW1340_pCAG-PV1-PhiC31-N233-L1-FRB-NLS-BGHpA

   Plasmid

   #108789

   Purpose

   Expresses N-terminus to aa233 of PhiC31 integrase fused to FRB CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   PhiC31 integrase fragment N-terminus to aa233
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   FRB,NLS

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBW1353_pCAG-PV1-FKBP-L1-PhiC31-234C-NLS-BGHpA

   Plasmid

   #108790

   Purpose

   Expresses aa234 to C-terminus of PhiC31 integrase fused to FKBP CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   PhiC31 integrase fragment aa234 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   FKBP and NLS

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBW2603_pCAG-PV1-iCre-N256-L1-ABI-NLS-BGHpA

   Plasmid

   #108743

   Purpose

   Expresses N-terminus to aa256 of Cre recombinase fused to ABI CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Cre recombinase fragment N-terminus to aa256
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   ABI,NLS

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBW2611_pCAG-PV1-PYL-L1-iCre-257C-NLS-BGHpA

    Plasmid

    #108744

    Purpose

    Expresses aa257 to C-terminus of Cre recombinase fused to PYL CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment aa257 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS and PYL

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-OSF-PP-ULK1(MIT)2, residues 833-1050

    Plasmid

    #180644

    Purpose

    Mammalian expression vector for ULK1 MIT domains, residues 833-1050. Has Precision protease cleavable N-terminal One-Strep Flag tag. Internal ID: WISP20-37.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    ULK1 (ULK1 Human)
    

    Tags

    One-Strep-Flag (OSF)

    Expression

    Mammalian

    Mutation

    Residues 833-1050

    Promoter

    pCAG

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBW2542_pCAG-PV1-VCre-N172-L1-GID1-NLS-BGHpA

    Plasmid

    #108771

    Purpose

    Expresses N-terminus to aa172 of VCre recombinase fused to GID1 CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    VCre recombinase fragment N-terminus to aa172
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GID1,NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBW2562_pCAG-PV1-GAI-L1-VCre-173C-NLS-BGHpA

    Plasmid

    #108772

    Purpose

    Expresses aa173 to C-terminus of VCre recombinase fused to GAI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    VCre recombinase fragment aa173 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GAI and NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCSD_2XNLS-SpCas9WT-NLS-3xHA-2XNLS-NmCas9WT-2XNLS

    Plasmid

    #107324

    Purpose

    Expresses SpCas9 fused to NmCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SpCas9-NmCas9 fusion
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    SpCas9 is fused to NmCas9

    Promoter

    CMV IE94

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. 221-pCAGGS-HA-AID-CTCFcDNA-AID-eGFP-2A-blasticidin

    Plasmid

    #195101

    Purpose

    Vector to introduce human CTCF cDNA tagged with eGFP and two AIDs in the cells. Blasticidin selection (2A-fusion). Auxin-inducible degron system.

    Depositor

    Job Dekker

    Article

    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

    Insert

    CTCF (CTCF Human)
    

    Tags

    AID, HA, and eGFP

    Expression

    Mammalian

    Promoter

    pCAGGS

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pspCas9-ABE-3'UTR-sgRNA-ST2-com vector

    Plasmid

    #136270

    Purpose

    Expresses ABE and ST2-com modified sgRNA scaffold

    Depositor

    Baisong Lu

    Article

    Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

    Insert

    ABE and sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-ABE8e-SpCas9-HiFi-P2A-EGFP (LM446)

    Plasmid

    #197506

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HiFi(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-HiFi-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-HiFi(D10A/R69…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBW2122_pCAG-PV1-GAI-L1-Bxb1-469C-NLS-BGHpA

    Plasmid

    #108816

    Purpose

    Expresses aa469 to C-terminus of Bxb1 integrase fused to GAI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Bxb1 integrase fragment aa469 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GAI and NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBW1599_pCAG-PV1-PhiC31-N233-L1-ABI-NLS-BGHpA

    Plasmid

    #108797

    Purpose

    Expresses N-terminus to aa233 of PhiC31 integrase fused to ABI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    PhiC31 integrase fragment N-terminus to aa233
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    ABI,NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBW2115_pCAG-PV1-PhiC31-N233-L1-GID1-NLS-BGHpA

    Plasmid

    #108805

    Purpose

    Expresses N-terminus to aa233 of PhiC31 integrase fused to GID1 CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    PhiC31 integrase fragment N-terminus to aa233
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GID1,NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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