We narrowed to 5,434 results for: kras

1. PX458-3xHA-en15FnCas9

   Plasmid

   #201948

   Purpose

   Mammalian expression plasmid of enhanced activity FnCas9 variant en15FnCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-en15FnCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   E1603H on FnCas9

   Promoter

   Cbh

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX4T1-TEV-HsFbxo7-129-398/Skp1

   Plasmid

   #202592

   Purpose

   Bacterial expression of HsFbxo7-129-398 and HsSkp1

   Depositor

   Brenda Schulman

   Article

   Kraus et al EMBO Rep. 2023 Aug 3;24(8):e56399. doi:

   Inserts

   Fbxo7 (FBXO7 Human)
   Skp1 (SKP1 Human)
   

   Use

   Tags

   GST, TEV

   Expression

   Bacterial

   Mutation

   aa 129-398 only

   Promoter

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. R713-M52-303: CMV51p> Halotag-NRasHVR

   Plasmid

   #159686

   Purpose

   Mammalian protein expression of NRAS HVR (Hypervariable region)-Halotag fusion

   Depositor

   Dominic Esposito

   Article

   Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

   Insert

   Halotag-NRasHVR (NRAS Human)
   

   Use

   Tags

   Halotag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. PX458-3xHA-FnCas9ABEmax8.17d

   Plasmid

   #201955

   Purpose

   Mammalian expression plasmid of FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   bpNLS-FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   D11A on FnCas9, V82S, V106W, Q154R on mutant TadA

   Promoter

   Cbh

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX304-BCL-XL-499/500R

   Plasmid

   #203575

   Purpose

   Expresses V5-tagged BCL-XL with resistance to shRNA (TRCN0000033499, TRCN0000033500) in mammalian cells.

   Depositor

   Abhishek Chakraborty

   Article

   Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

   Insert

   BCL2L1 (BCL2L1 Human)
   

   Use

   Lentiviral

   Tags

   V5-tagged

   Expression

   Mammalian

   Mutation

   silent point mutations to make resistant shRNA 49…

   Promoter

   CMV

   Available since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. CRISPRoff-mScarletI

   Plasmid

   #217783

   Purpose

   Expresses CRISPRoff-mScarletI (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-mScarletI-KRAB) downstream of the CAG promoter for gene epigenetic silencing

   Depositor

   Adriano Aguzzi

   Article

   Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

   Insert

   CRISPRoff-mScarletI (DNMT3A-DNMT3L-dCas9-mScarletI-KRAB)
   

   Use

   Tags

   2xNLS, DNMT3A-DNMT3L, HA, KRAB, and mScarletI

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT/CAGGS-NRASG12V-IRES-mVenus

   Plasmid

   #236076

   Purpose

   Sleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Caggs promotor driven expression of mVenus and mutant NRAS (NRASG12V).

   Depositor

   Masashi Narita

   Article

   Chan et al Nature. 2024 Sep;633(8030):678-685. doi:

   Inserts

   mVenus
   NRAS G12V (NRAS Human)
   

   Use

   Sleeping beauty transposon plasmid

   Tags

   Expression

   Mutation

   G12V

   Promoter

   CAGGS

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT/CAGGS-mVenus-P2A-NRASG12V

   Plasmid

   #236072

   Purpose

   Sleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Caggs promotor driven expression of mVenus and mutant NRAS (NRASG12V).

   Depositor

   Masashi Narita

   Article

   Chan et al Nature. 2024 Sep;633(8030):678-685. doi:

   Inserts

   mVenus
   NRAS G12V (NRAS Human)
   

   Use

   Sleeping beauty transposon plasmid

   Tags

   Expression

   Mutation

   G12V

   Promoter

   CAGGS

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. FLINC-AKAR1-CAAX

   Plasmid

   #87705

   Purpose

   Fluorescent biosensor for super-resolution imaging of PKA activity; targeted to plasma membrane.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

   Insert

   FLINC-AKAR1-CAAX
   

   Use

   Tags

   C-terminal targeting sequence from Kras

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. PKAsub-TagRFPT-CAAX

    Plasmid

    #87712

    Purpose

    Encodes C-terminal (substrate) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR); targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

    Insert

    PKAsub-TagRFPT-CAAX
    

    Use

    Tags

    6xHis, C-terminal targeting sequence from Kras, T…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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