We narrowed to 21,033 results for: Vars

1. TagRFP-T-EEA1

   Plasmid

   #42635

   Depositor

   Silvia Corvera

   Article

   Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

   Insert

   EEA1 (EEA1 Human)
   

   Tags

   TagRFP-T

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   March 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. xCas9

   Plasmid

   #138565

   Purpose

   Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   xCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)- e18

   Plasmid

   #131670

   Purpose

   Expresses an engineered variant of RAD18 ("e18") that stimulates CRISPR mediated HDR.

   Depositor

   Alberto Ciccia

   Article

   Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

   Insert

   e18 (engineered RAD18 with SAP domain deleted) (RAD18 Human)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. RET-V5

   Plasmid

   #139627

   Purpose

   V5-His-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

   Insert

   RET (RET Human)
   

   Tags

   3xV5-6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETM33_CRK_SH3

   Plasmid

   #178472

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   CRK_SH3 (CRK Human, Synthetic)
   

   Tags

   His-GST

   Expression

   Bacterial

   Promoter

   T7/LacO

   Available Since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBJ5 hBRG1

   Plasmid

   #17873

   Depositor

   Jerry Crabtree

   Article

   Khavari et al Nature. 1993 Nov 11. 366(6451):170-4.

   Insert

   Brg (SMARCA4 Human)
   

   Expression

   Mammalian

   Mutation

   R1608Q

   Available Since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. MAC_N_GATA4

   Plasmid

   #167758

   Purpose

   Expresses GATA4 in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   GATA4 (GATA4 Human)
   

   Tags

   MAC

   Expression

   Mammalian

   Mutation

   no mutations

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. p3R112C/T180CBM3

   Plasmid

   #85090

   Purpose

   Expresses PCNA3 variant (R112C/T180C) fused to P450 BM3 variant (A74G/C773S/C810S) in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   The R112C/T180C variant of PCNA3 fused to P450 BM3
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   R112C/T180C in PCNA3, A74G/C773S/C810S in P450BM3

   Promoter

   T7 promoter

   Available Since

   Dec. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. BirA-Myc_N_ETS1

   Plasmid

   #167700

   Purpose

   Expresses ETS1 in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   ETS1 (ETS1 Human)
   

   Tags

   Myc-BirA

   Expression

   Mammalian

   Mutation

   no mutations

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV Syn CaMPARI2-human-a-Tubulin (TubuTag)

    Plasmid

    #164184

    Purpose

    Freeze-frame imaging of dendritic calcium signals CaMPARI2 moiety with lower Ca+ affinity (Kd=199nM) (Moeyaert et al., 2018)

    Depositor

    Thomas Oertner

    Article

    Perez-Alvarez et al Front Mol Neurosci. 2021 Mar 4;14:635820

    Inserts

    CamPARI2
    Human α Tubulin
    

    Use

    AAV

    Tags

    6 His tag and NES

    Expression

    Mammalian

    Promoter

    Syn

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDFNKI-strepII-3C-LIC-strep

    Plasmid

    #108708

    Purpose

    Expression of a StrepII-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Tags

    StrepII-3C protease cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1P shSOD1

    Plasmid

    #102975

    Purpose

    Suppression of SOD1

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shSOD1 (SOD1 Human)
    

    Use

    Lentiviral and RNAi

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-TetON-dual-SunTag-Hygro

    Plasmid

    #121119

    Purpose

    Endogenous gene activation with the SunTag CRISPR activation system

    Depositor

    Pablo Navarro

    Article

    Heurtier et al Nat Commun. 2019 Mar 7;10(1):1109. doi:

    Insert

    dCas9-GCN4x10_scFv-VP64
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Tet-ON

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. PM-FRB-CFP

    Plasmid

    #67517

    Purpose

    FRB conjugated plasma membrane targeting sequences from GAP43 (MLCCMRRTKQVEKNDDQKI)

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

    Insert

    mTOR (MTOR Human)
    

    Tags

    CFP, HA, and PM tethering sequence (MLCCMRRTKQVEK…

    Expression

    Mammalian

    Mutation

    FRB domain only (residues 2019–2114)

    Promoter

    CMV

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

15. MAC_N_ETV4

    Plasmid

    #167753

    Purpose

    Expresses ETV4 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    ETV4 (ETV4 Human)
    

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. PTPRA-SHC

    Plasmid

    #139611

    Purpose

    StrepIII-HA-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

    Insert

    PTPRA (PTPRA Human)
    

    Tags

    StrepIII-HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. ETV5_pet28a

    Plasmid

    #131639

    Purpose

    Bacterial expression of ETS gene ETV5

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ETV5 (ETV5 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETNKI-strepII-3C-LIC-Amp

    Plasmid

    #108707

    Purpose

    Expression of a StrepII-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Tags

    StrepII-3C protease cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTPH413

    Plasmid

    #185728

    Purpose

    Bacterial PAM profiling of adenine base editor variants

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Inserts

    ecTadA(8e)-neNme2-C
    Nme2Cas9 sgRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

    Available Since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. CDK1as_T2A_Zeo

    Plasmid

    #118596

    Purpose

    One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.

    Depositor

    William Earnshaw

    Article

    Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

    Insert

    CDK1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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