We narrowed to 2,781 results for: ada.2

Showing: 2681 - 2700 of 2781 results

Results per page:

Sort results by:

pFBD-BirA_LRRK2:2141-2527

Plasmid

#210901

Purpose

Insect expression of human LRRK2 fragment T2141 to E2527. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

LRRK2:T2141-E2527 (LRRK2 Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

M2397T natural variant ( VAR_024965)

Promoter

Polyhedrin

Available Since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBacMam2-DiEx-LIC_LRRK2:2141-2527

Plasmid

#210900

Purpose

Insect expression of human LRRK2 fragment T2141 to E2527. C-terminal 10X His and Flag tag with TEV cleavage site.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

LRRK2:T2141-E2527 (LRRK2 Human)

Tags

AENLYFQSHHHHHHHHHHDYKDDDDK and M

Expression

Insect

Mutation

M2397T natural variant ( VAR_024965)

Promoter

P10 and CMV

Available Since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pFBD-BirA_CDC40:236-578

Plasmid

#210885

Purpose

Insect expression of human CDC40 fragment E236 to W578. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CDC40:E236-W578 (CDC40 Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Cherry-HA-KCC2

Plasmid

#104077

Purpose

To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)

Depositor

Igor Medyna

Article

Kahle et al EMBO Rep. 2014 Jul;15(7):766-74. doi: 10

Insert

KCC2 (b isoform) (Slc12a5 Rat)

Tags

mCherry-HA tag

Expression

Mammalian

Mutation

EcoR1 site in rat KCC2 was removed by silent muta…

Promoter

UbC

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BCL6-GFP (1-275AA) in Cilantro

Plasmid

#169932

Purpose

Overexpression of 1-275AA BCL6-GFP fusion

Depositor

Benjamin Ebert

Article

Slabicki et al Nature. 2020 Dec;588(7836):164-168. doi:

Insert

BCL6 1-275AA (BCL6 Human)

Use

Lentiviral

Tags

GFP

Mutation

160R>C

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
W118-1_hRSK3(RPS6KA2)_K91A_K444A

Plasmid

#180385

Purpose

lentiviral transduction of human RSK3 (RPS6KA2) gene with point mutations (K91A and K444A) that inactivate kinase activity

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

RPS6KA2_K91A/K444A (RPS6KA2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

K91A/K444A

Promoter

CMV

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CL20_mEGFP_THSD4_LRRC49

Plasmid

#205941

Purpose

Express mEGFP-tagged fusion protein, THSD4_LRRC49 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

THSD4_LRRC49 (THSD4, LRRC49 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Phage-PGK-Flag-HA-FKBP-T(G177D)

Plasmid

#181735

Purpose

for lentiviral expression of brachyury ORF in mammalian cells

Depositor

Charles Lin

Article

Sheppard et al Cell Rep Med. 2021 Jan 19;2(1):100188. d

Insert

T (brachyury)

Use

Lentiviral

Mutation

a silent mutation in the PAM site of an sgRNAt ta…

Available Since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCMV-T7-NLS(SV40)-(Spyo_Cas9)-6xHis-NLS(SV40)

Plasmid

#185706

Purpose

CMV and T7 promoter expression plasmid for human codon usage anCas(Spyo_Cas9) with an N-terminal NLS and C-terminal NLS

Depositor

Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

Cas9

Tags

6xHis-NLS and NLS

Expression

Mammalian

Mutation

optimized for human codon usage

Promoter

CMV and T7

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-noSV40-humanEC1.45_min1-2_1XCoordinatorMutant

Plasmid

#173963

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 1 mutated Coordinator motif

Use

Luciferase

Mutation

1 mutated Coordinator motif

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GSO2/EDA23 X079_pECIA2

Plasmid

#115111

Purpose

Bait vector GSO2/EDA23 X079_pECIA2 should be used with prey vector GSO2/EDA23 X079_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G44700 (GSO2 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-NLS(SV40)-anCas(FCA)-6xHis-NLS(SV40)

Plasmid

#185701

Purpose

CMV and T7 promoter expression plasmid for human codon usage anCas(FCA) with an N-terminal NLS and C-terminal NLS

Depositor

Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

anCas(FCA)

Tags

6xHis-NLS and NLS

Expression

Mammalian

Mutation

optimized for human codon usage

Promoter

CMV and T7

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-NLS(SV40)-anCas(PDCA)-6xHis-NLS(SV40)

Plasmid

#185705

Purpose

CMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS and C-terminal NLS

Depositor

Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

anCas(PDCA)

Tags

6xHis-NLS and NLS

Expression

Mammalian

Mutation

optimized for human codon usage

Promoter

CMV and T7

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-NLS(SV40)-anCas(SCA)-6xHis-NLS(SV40)

Plasmid

#185703

Purpose

CMV and T7 promoter expression plasmid for human codon usage anCas(SCA) with an N-terminal NLS and C-terminal NLS

Depositor

Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

anCas(SCA)

Tags

6xHis-NLS and NLS

Expression

Mammalian

Mutation

optimized for human codon usage

Promoter

CMV and T7

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-NLS(SV40)-anCas(PCA)-6xHis-NLS(SV40)

Plasmid

#185704

Purpose

CMV and T7 promoter expression plasmid for human codon usage anCas(PCA) with an N-terminal NLS and C-terminal NLS

Depositor

Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

anCas(PCA)

Tags

6xHis-NLS and NLS

Expression

Mammalian

Mutation

optimized for human codon usage

Promoter

CMV and T7

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-NLS(SV40)-anCas(BCA)-6xHis-NLS(SV40)

Plasmid

#185702

Purpose

CMV and T7 promoter expression plasmid for human codon usage anCas(BCA) with an N-terminal NLS and C-terminal NLS

Depositor

Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

anCas(BCA)

Tags

6xHis-NLS and NLS

Expression

Mammalian

Mutation

optimized for human codon usage

Promoter

CMV and T7

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-noSV40-EC1.45-human_min1-opossum_min2

Plasmid

#173966

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Human minimal enhancer region 1 (min1) and opossum minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

See Depositor Comments Below

Promoter

SV40 promoter

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-noSV40-humanEC1.45_min1-2_4XCoordinatorMutant

Plasmid

#173962

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 4 mutated Coordinator motifs

Use

Luciferase

Mutation

4 mutated Coordinator motifs

Promoter

SV40 promoter

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-noSV40-humanEC1.45_p300peak1-2_CoordinatorMutant#1

Plasmid

#173985

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motif #1 mutated

Use

Luciferase

Mutation

Coordinator motif mutation #1

Promoter

SV40 promoter

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-noSV40-humanEC1.45_p300peak1-2_CoordinatorMutant#3

Plasmid

#173987

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motif #3 mutated

Use

Luciferase

Mutation

Coordinator motif mutation #3

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge