We narrowed to 23,624 results for: crispr

1. pEJS583-pCSDest2-AcrIIC5Smu-FLAG-NLS

   Plasmid

   #113437

   Purpose

   Expresses a FLAG/NLS tagged type II-C anti-CRISPR protein from S. muelleri in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

   Insert

   Type II-C anti-CRISPR
   

   Use

   CRISPR

   Tags

   FLAG/NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWT-IFl_WT-IFr

   Plasmid

   #192207

   Purpose

   Wildtype I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Wildtype I-F Leader and wildtype I-F Locus DNA
   

   Expression

   Bacterial

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pScr-IFl_WT-IFr

   Plasmid

   #192208

   Purpose

   Scrambled I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Scrambled I-F Leader and wildtype I-F Locus DNA
   

   Expression

   Bacterial

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV Intein-C-SpyCas9, CMV-AcrIIA4-2xmiR-122 target sites

   Plasmid

   #120295

   Purpose

   AAV Vector for expression of C-terminal SpyCas9 fragement with split-intein and a CMV-driven AcrIIA4 with two miR-122 binding sites

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   C-terminal fragment of SpyCas9
   

   Use

   AAV and CRISPR

   Tags

   split-intein

   Expression

   Mammalian

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSPneoHHgRNAH

   Plasmid

   #63557

   Purpose

   Expresses gRNA in Leishmania

   Depositor

   Greg Matlashewski

   Article

   Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

   Insert

   guide sequence
   

   Use

   Leishmania expression

   Available Since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pgRNA_AT

   Plasmid

   #196243

   Purpose

   Backbone plasmid for cloning sgRNA

   Depositor

   Andrea Puhar

   Article

   Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRIM3-rfp

   Plasmid

   #74073

   Purpose

   GentR version of pRIM2 carrying rfp

   Depositor

   Timothy Lu

   Article

   Cleto et al ACS Synth Biol. 2016 Feb 16.

   Insert

   rfp
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   ptac

   Available Since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. hCtRNA_FT

   Plasmid

   #186715

   Purpose

   Fragment template for DAP array

   Depositor

   Xue Gao

   Article

   Yuan et al Nat Commun. 2022 May 19;13(1):2771. doi:

   Insert

   gRNA scaffold and hCtRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hCtRNA

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAW005-2

   Plasmid

   #85611

   Purpose

   Empty gRNA delivery vector for Bacillus subtilis

   Depositor

   Perry Chou

   Article

   Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Jan. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. hCtRNA_VT

    Plasmid

    #186716

    Purpose

    Vector template for DAP array

    Depositor

    Xue Gao

    Article

    Yuan et al Nat Commun. 2022 May 19;13(1):2771. doi:

    Insert

    hCtRNA and gRNA scaffold
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hCtRNA

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. EC67842

    Plasmid

    #209516

    Purpose

    Cloning in complete guide expression cassettes, with Cas12a (D156R)

    Depositor

    Cristobal Uauy, Wendy Harwood

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. EC67841

    Plasmid

    #209512

    Purpose

    Cloning in complete guide expression cassettes, with Cas12a (D156R)

    Depositor

    Cristobal Uauy, Wendy Harwood

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. EC67843

    Plasmid

    #209513

    Purpose

    Cloning in complete guide expression cassettes, with Cas12a (D156R)

    Depositor

    Cristobal Uauy, Wendy Harwood

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CMV-FLEX-SaCas9-U6-sgKcnq3

    Plasmid

    #159911

    Purpose

    Mutagenesis of Kcnq3

    Depositor

    Larry Zweifel

    Insert

    Kcnq3 (Kcnq3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX20

    Plasmid

    #169443

    Purpose

    Used for guide cloning with PCR

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXZ13lacZalpha

    Plasmid

    #160229

    Purpose

    places LacZalpha unit between Esp3I sites for better cutting of plasmid and ability to distinguish recombinants as white colonies when plated on Xgal plates

    Depositor

    Ian Duncan

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX52

    Plasmid

    #169444

    Purpose

    Used for guide cloning with PCR

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBS-GGAC-ATGC

    Plasmid

    #60949

    Purpose

    Drosophila, backbone for donor plasmid assembly. CRISPR knock-in.

    Depositor

    Frank Schnorrer

    Article

    Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSPneogRNAH

    Plasmid

    #63556

    Purpose

    Expresses gRNA in Leishmania

    Depositor

    Greg Matlashewski

    Article

    Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

    Insert

    guide sequence
    

    Use

    Leishmania expression

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBS-GGAC-TTCT

    Plasmid

    #60945

    Purpose

    Drosophila, backbone for donor plasmid assembly. CRISPR knock-in.

    Depositor

    Frank Schnorrer

    Article

    Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge