We narrowed to 3,105 results for: pcdna3 1

1. pcDNA-GFP-EBA175-Flag(Y1426I)

   Plasmid

   #154976

   Purpose

   Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 Y1426I variant (residues 1284-1462) from pcDNA3.1

   Depositor

   Sinisa Urban

   Article

   Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

   Insert

   PfEBA175
   

   Use

   Tags

   Flag, GFP, and signal peptide (residues 1 to 32) …

   Expression

   Mammalian

   Mutation

   Y1426I; insert codes for EBA175 residues 1284 to …

   Promoter

   CMV

   Available since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. InsRA-eGFP

   Plasmid

   #79795

   Purpose

   Insulin receptor isoform A with a inter-domain eGFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.

   Depositor

   James Johnson

   Article

   Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

   Insert

   InsRA (INSR Human)
   

   Use

   Tags

   eGFP extracellular

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. InsRA-TagBFP

   Plasmid

   #79791

   Purpose

   Insulin receptor isoform A with a inter-domain TagBFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.

   Depositor

   James Johnson

   Article

   Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

   Insert

   InsRA (INSR Human)
   

   Use

   Tags

   TagBFP extracellular

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 5, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. InsRA-EBFP2

   Plasmid

   #79797

   Purpose

   Insulin receptor isoform A with a inter-domain EBFP2 tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.

   Depositor

   James Johnson

   Article

   Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

   Insert

   InsRA (INSR Human)
   

   Use

   Tags

   EBFP2 extracellular

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 5, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. BE3-NLS-NLS-P2A-BLAST

   Plasmid

   #135348

   Purpose

   Expression of BE3 (Base editor generation three) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   BE3-2XNLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. S1 1029 CP APOBEC3 no UGI

   Plasmid

   #135352

   Purpose

   Expression of circular permutant of nSpyCas9 lacking uracil DNA glycosylase inhibitor at amino acid position 1029 encoding rat cytosine deaminase, rAPOBEC3 at the N-terminus

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   rAPOBEC3-nSpyCas9 aa 1029
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. S1 1029 CP ABEMax strategy 2

   Plasmid

   #135358

   Purpose

   Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding de-coupled ABEMax (wildtype Tada monomer at N terminus, evolved Tada monomer at C terminus)

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   WT Tada-nSpyCas9 aa 1029-evolved Tada
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. DDX41-GFP

   Plasmid

   #175494

   Purpose

   Total RNA was isolated from TF-1 cells and converted to cDNA using SuperScript III (Invitrogen). The full DDX41 coding sequence was cloned into pcDNA3.1 (Invitrogen) with an EGFP c-terminal insert.

   Depositor

   Evan Braunstein

   Article

   Li et al Haematologica. 2016 Jun;101(6):e228-31.

   Insert

   DDX41 (DDX41 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA-GFP-EBA175-Flag(M1423R/P1424V)

   Plasmid

   #155309

   Purpose

   Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 M1423R/P1424V variant (residues 1284-1462) from pcDNA3.1

   Depositor

   Sinisa Urban

   Article

   Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

   Insert

   PfEBA175
   

   Use

   Tags

   Flag, GFP, and signal peptide (residues 1 to 32) …

   Expression

   Mammalian

   Mutation

   M1423R/P1424V; insert codes for EBA175 residues 1…

   Promoter

   CMV

   Available since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-GFP-EBA175-Flag(P1424V/Y1425W)

    Plasmid

    #155310

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424V/Y1425W variant (residues 1284-1462) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    P1424V/Y1425W; insert codes for EBA175 residues 1…

    Promoter

    CMV

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA-GFP-EBA175-Flag(P1424V/Y1426H)

    Plasmid

    #155311

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424V/Y1426H variant (residues 1284-1462) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    P1424V/Y1426H; insert codes for EBA175 residues 1…

    Promoter

    CMV

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA-GFP-EBA175-Flag(P1424Q/Y1426S)

    Plasmid

    #155003

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    P1424Q/Y1426S; insert codes for EBA175 residues 1…

    Promoter

    CMV

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA-GFP-EBA175-Flag(P1424R/Y1426S)

    Plasmid

    #155004

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424R/Y1426S variant (residues 1284-1462) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    P1424R/Y1426S; insert codes for EBA175 residues 1…

    Promoter

    CMV

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA-GFP-EBA175-Flag(P1424D/Y1426S)

    Plasmid

    #155005

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424D/Y1426S variant (residues 1284-1462) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    P1424D/Y1426S; insert codes for EBA175 residues 1…

    Promoter

    CMV

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA-GFP-EBA175-Flag(E1418K/P1424Q/Y1426S)

    Plasmid

    #155006

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    E1418K/P1424Q/Y1426S; insert codes for EBA175 res…

    Promoter

    CMV

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA-GFP-EBA175-Flag(M1423R/P1424V/Y1426H)

    Plasmid

    #155312

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 M1423R/P1424V/Y1426H variant (residues 1284-1462) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    M1423R/P1424V/Y1426H; insert codes for EBA175 res…

    Promoter

    CMV

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA-GFP-EBA175-Flag(A1419R/P1424Q/Y1426S)

    Plasmid

    #155007

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 A1419R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    A1419R/P1424Q/Y1426S; insert codes for EBA175 res…

    Promoter

    CMV

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA-GFP-EBA175-Flag(S1421R/P1424Q/Y1426S)

    Plasmid

    #155008

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 S1421R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    S1421R/P1424Q/Y1426S; insert codes for EBA175 res…

    Promoter

    CMV

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA-GFP-EBA175-Flag(P1424I/Y1425I/Y1426I)

    Plasmid

    #154977

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424A/Y1425I/Y1426I variant (residues 1284-1462) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    P1424/Y1425I/Y1426I; insert codes for EBA175 resi…

    Promoter

    CMV

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NSP4-WT-Flag

    Plasmid

    #210339

    Purpose

    Expresses SARS-CoV-2 NSP4 fused with Flag in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge