We narrowed to 10,541 results for: yeast expression

1. pMEL11

   Plasmid

   #107917

   Purpose

   amdS based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pWR63

   Plasmid

   #203331

   Purpose

   episomal Cas9 and sgRNA expression vector without sgRNA for S. stipitis

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
   coHPH
   ACT1 terminator (S. stipitis)
   CCW12 promoter (S. stipitis)
   CaCas9
   ADH2 terminator (S. stipitis)
   ARS from S. stipitis chromosome 1
   THD3 promoter (S. stipitis)
   tRNA-sgRNA(SapI)-HDV
   ADH1 terminator (S. stipitis)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PCUP1-GFP-Pho8-406

   Plasmid

   #207006

   Purpose

   Integration of GFP-Pho8 as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Marker for yeast vacuolar membrane.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   PHO8
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PCUP1-GFP-Osh1-406

   Plasmid

   #207007

   Purpose

   Integration of Osh1-GFP as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Yeast PMN pathway substrate.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   OSH1
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGADT7-δ

   Plasmid

   #197259

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-3 δ fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

   Insert

   AP-3 δ (AP3D1 Human)
   

   Use

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   Val 549 Ile substitution; silent substitutions in…

   Promoter

   ADH1

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGADT7-γ1

   Plasmid

   #197078

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-1 γ1 fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

   Insert

   AP-1 γ1 (Ap1g1 Mouse)
   

   Use

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGADT7-ε

   Plasmid

   #197261

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-4 ε fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

   Insert

   AP-4 ε (AP4E1 Human)
   

   Use

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   silent substitutions in codons 905, 1129 and 1137

   Promoter

   ADH1

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. deltaVP1 + VP2C-GFP-MIOX

   Plasmid

   #166682

   Purpose

   Yeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase fused to VP2C-GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

   Inserts

   VP2C-GFP-MIOX
   Murine polyomavirus deltaVP1 (NLS deletion mutant)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 and GAL10

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MIOX only

   Plasmid

   #166681

   Purpose

   Yeast integrative plasmid for expressing mouse myo-inositol oxygenase (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

   Insert

   MIOX (Miox Mouse, Budding Yeast, Synthetic)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL10

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. wtVP1 + VP2C-GFPdeg

    Plasmid

    #166678

    Purpose

    Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1 (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Inserts

    VP2C-GFPdeg
    Wild-type Murine polyomavirus VP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL10

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. deltaVP1 + VP2C-GFPdeg

    Plasmid

    #166677

    Purpose

    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Inserts

    VP2C-GFPdeg
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL10

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. wtVP1 + VP2C-GFP

    Plasmid

    #166674

    Purpose

    Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Inserts

    VP2C-GFP
    Wild-type Murine polyomavirus VP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL10

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCu-His6-Atg13[571–700] K683E, H685E, K686E-Ss(424)

    Plasmid

    #166855

    Purpose

    Expresses 6xHis tagged ATG13 [571–700] fragment mutated in K683E, H685E, K686E with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

    Insert

    Atg13[571–700] (ATG13 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    K683E, H685E, K686E

    Promoter

    pCu

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pZ_lacO-cP-AOX1-sLovA

    Plasmid

    #126741

    Purpose

    yeast plasmid overexpressing sLovA

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    slovA from Aspergillus terreus ATCC 20542 (LovA) codon optimized for expression in Komagataella phaffii
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAV10.F3.loxP

    Plasmid

    #63200

    Purpose

    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAV10.K6.loxP

    Plasmid

    #63203

    Purpose

    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAV10.HO5.loxP

    Plasmid

    #63206

    Purpose

    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pND003

    Plasmid

    #200949

    Purpose

    Yeast surface display destination vector for Golden Gate cloning

    Depositor

    Timothy Whitehead

    Article

    Daffern et al Biotechnol Bioeng. 2024 Jan;121(1):281-2

    Type

    Empty backbone

    Use

    Yeast surface display

    Tags

    AGA2, HA, and Myc

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    June 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFA6a URA3

    Plasmid

    #61924

    Purpose

    Expression of S. cerevisiae URA3 cloned into pFA6a

    Depositor

    David Tollervey, Jon Houseley

    Article

    Houseley et al Nucleic Acids Res. 2011 Nov 1;39(20):877

    Insert

    URA3
    

    Use

    Yeast genomic targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NM R2E2 GFP

    Plasmid

    #1208

    Depositor

    Susan Lindquist

    Article

    Liu et al Nature 1999 Aug 5;400(6744):573-6.

    Insert

    sup35 NM domain (SUP35 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    2 additional copies of second oligopeptide repeat…

    Promoter

    Available Since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge