We narrowed to 3,637 results for: golden gate

1. pCFJ1358 - Intron GG1 - smu-1

   Plasmid

   #159804

   Purpose

   Golden Gate compatible intron with PATCs for reduced germline silencing

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   Intron GG1 - smu-1
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCFJ2214 - Intron GG1 - 900 bp

   Plasmid

   #159880

   Purpose

   Golden Gate compatible intron with PATCs for reduced germline silencing

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   Intron GG1 - 900 bp
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEPOR1CB0112

   Plasmid

   #117548

   Purpose

   Level 1 Golden Gate Cassette: xCas9 3.7 expression cassette for dicotyledonous plants

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   2x35S+5'UTR OMEGA (pICH51288) + Sp-xCas9 3.7 (no stop codon) (pEPOR0SP0011) + YFP (pICSL50005) + Nos (pICH41421)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   2x35S+5'UTR OMEGA (pICH51288)

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZA27

   Plasmid

   #158505

   Purpose

   In planta gene expression of Golden gate compatible A. thaliana ZAR1 L17E-4xMyc. Km plant selection marker.

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   ZAR1-4xMyc (ZAR1 Mustard Weed)
   

   Use

   Tags

   4xMyc

   Expression

   Plant

   Mutation

   L17E

   Promoter

   Available since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. p230-pOsAct1::hpt-pZmUbi::BdPetC

   Plasmid

   #129649

   Purpose

   Rieske FeS overexpression

   Depositor

   Susanne von Caemmerer

   Article

   Ermakova et al Commun Biol. 2019 Aug 16;2:314. doi: 10.

   Insert

   CDS of PetC gene encoding for the Rieske FeS subunit
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Codon modified for Golden Gate

   Promoter

   pZmUbi

   Available since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. SMT503

   Plasmid

   #226840

   Purpose

   Plasmid that encodes a B. Subtilis lipase (BsLipA) gene fusion with N-terminal SNAP and C-Terminal eGFP under a T7 promoter. Compatible with PURExpress for in vitro transcription/translation. Chloramp

   Depositor

   Polly Fordyce

   Article

   Thompson et al Adv Mater Interfaces 2024, 12 (3), 24004

   Insert

   Lipase A
   

   Use

   Synthetic Biology

   Tags

   SNAP and eGFP

   Expression

   Bacterial

   Mutation

   R33Q, D34N, K35D, K112D, M134D, Y139C, I157M

   Promoter

   T7

   Available since

   Aug. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNS1D-sgRNA9-SpR

   Plasmid

   #191092

   Purpose

   aTc inducible gRNA expression plasmid with BbsI sites for Golden Gate insertion of crRNA oligos and homology arms for genome integration at NS1D in Synechococcus sp. PCC 7002

   Depositor

   Anne Ruffing

   Article

   Dallo et al ACS Synth Biol. 2023 Apr 21;12(4):1175-1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tet

   Available since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSEVAb827

   Plasmid

   #217500

   Purpose

   pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb82 backbone, RK2 ori

   Depositor

   Nico Claassens

   Article

   van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

   Insert

   superfolder green fluorescent protein (sfGFP)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

   Promoter

   Available since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSEVAb837

   Plasmid

   #217501

   Purpose

   pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb83 backbone, pBBR1 ori

   Depositor

   Nico Claassens

   Article

   van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

   Insert

   superfolder green fluorescent protein (sfGFP)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

   Promoter

   Available since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSEVAb847

    Plasmid

    #217502

    Purpose

    pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb84 backbone, pRO1600/ColE1 ori

    Depositor

    Nico Claassens

    Article

    van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

    Insert

    superfolder green fluorescent protein (sfGFP)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

    Promoter

    Available since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSEVAb867

    Plasmid

    #217503

    Purpose

    pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb86 backbone, p15A ori

    Depositor

    Nico Claassens

    Article

    van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

    Insert

    superfolder green fluorescent protein (sfGFP)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

    Promoter

    Available since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSEVAb887

    Plasmid

    #217504

    Purpose

    pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb88 backbone, pUC ori

    Depositor

    Nico Claassens

    Article

    van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

    Insert

    superfolder green fluorescent protein (sfGFP)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

    Promoter

    Available since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSEVAb897

    Plasmid

    #217505

    Purpose

    pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb89 backbone, pBR322/ROP ori

    Depositor

    Nico Claassens

    Article

    van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

    Insert

    superfolder green fluorescent protein (sfGFP)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

    Promoter

    Available since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAD183_gCAD_pET

    Plasmid

    #239119

    Purpose

    Gorilla ACOD1 in E.coli expression vector

    Depositor

    Konrad Buessow

    Article

    Chen et al Sci Rep. 2023 Jun 26;13(1):10360. doi: 1

    Insert

    ACOD1
    

    Use

    Tags

    StrepTag-II and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKG3668_pGEEC578_pShip-EFS-rtTA-P2A-iRFP720-bGH

    Plasmid

    #239741

    Purpose

    Plasmid expressing a doxycycline-inducible transcriptional activator

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    rtTA-P2A-iRFP720
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKG2499_pGEEC531_pShip-CMV-mRuby2-WPRE

    Plasmid

    #239727

    Purpose

    Plasmid expressing mRuby2 with the CMV promoter and WPRE 3' UTR

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKG2906_pGEEC546_pShip-EFS-tagBFP-bGH

    Plasmid

    #239734

    Purpose

    Plasmid expressing tagBFP with the EFS promoter

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    tagBFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKG2907_pGEEC547_pShip-hPGK-tagBFP-bGH

    Plasmid

    #239735

    Purpose

    Plasmid expressing tagBFP with the hPGK promoter

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    tagBFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKG2897_pGEEC548_pShip-UbC-tagBFP-bGH

    Plasmid

    #239736

    Purpose

    Plasmid expressing tagBFP with the UbC promoter

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    tagBFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKG2899_pGEEC550_pShip-CMV-tagBFP-bGH

    Plasmid

    #239737

    Purpose

    Plasmid expressing tagBFP with the CMV promoter

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    tagBFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge