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pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA

Plasmid

#37120

Purpose

DO/DIO Cre-Switch. Expresses tdTomato in Cre negative cells, expresses EGFP in Cre positive cells

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

TdTomato-EGFP

Use

AAV and Cre/Lox; Cre-switch

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-NLS-mKate2-WPREpA

Plasmid

#84474

Purpose

pAAV-RAM-NLS-mKate2

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Insert

mKate2

Use

AAV

Tags

Nuclear localization signal of SV40 large T antig…

Promoter

TRE

Available Since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pTP396

Plasmid

#149336

Purpose

Bacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobody

Depositor

Rebecca Voorhees

Article

Pleiner et al Science. 2020 May 21. pii: science.abb50

Insert

anti-GFP nanobody

Tags

Biotin acceptor peptide (Avi-tag), His14, and SUM…

Expression

Bacterial

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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iCas

Plasmid

#84232

Purpose

Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)

Depositor

Meng How Tan

Article

Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038

Insert

Cas9

Tags

2A-OFP co-expression and ERT2-ERT2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pGEMHE-vhhGFP4-hIgG1-Fc

Plasmid

#105576

Purpose

T7 promotor drives in vitro mRNA transcription of GFP nanobody-Fc antibody domain fusion

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

Human IgG1 Fc-domain fused with GFP nanobody (IGHG1 Human)

Use

In vitro transcription

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-cyto-GCaMP6f

Plasmid

#52925

Purpose

Fluorescent reporter for calcium signaling

Depositor

Baljit Khakh

Article

Haustein et al Neuron. 2014 Apr 16;82(2):413-29. doi: 1

Has Service

AAV5

Insert

GCaMP6f

Use

AAV

Available Since

June 23, 2014

Availability

Academic Institutions and Nonprofits only

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pGL3 NSE promoter

Plasmid

#11606

Depositor

Rosalyn Adam

Article

Kim et al Endocrinology. 2004 Feb . 145(2):613-9.

Insert

NSE promoter (ENO2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

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psfaRb-Tn5

Plasmid

#183638

Purpose

Expresses anti-rabbit nanobody fused with Tn5

Depositor

Goncalo Castelo-Branco

Article

Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

Insert

anti rabbit secondary nanobody fused to Tn5

Expression

Bacterial

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pMLM800

Plasmid

#27202

Depositor

Keith Joung

Article

Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

Insert

FokI endonuclease

Use

Zebrafish expression

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pMLM802

Plasmid

#27203

Depositor

Keith Joung

Article

Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

Insert

FokI endonuclease

Use

Zebrafish expression

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 18, 2011

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-NICD

Plasmid

#61540

Purpose

Expresses NOTCH-ICD in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

NOTCH-ICD (NOTCH1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

NOTCH intracellular domain (aa1761-2555); A1943S…

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-myc T58A

Plasmid

#19763

Depositor

Konrad Hochedlinger

Article

Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.

Insert

c-myc (MYC Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

T58A

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Antares2 c-myc

Plasmid

#100027

Purpose

A BRET based CyOFP-teLuc-CyOFP fusion to further red-shift bioluminescence

Depositor

Huiwang Ai

Article

Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

Insert

Antares2

Tags

c-myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-rev-ChR2(H134R)-mCherry

Plasmid

#18916

Purpose

Cre-dependent viral expression of ChR2(H134R)-mCherry for neuronal excitation

Depositor

Scott Sternson

Article

Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

Insert

Channelrhodopsin 2-mCherry

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

mCherry

Mutation

H134R

Promoter

CAG

Available Since

Jan. 29, 2009

Availability

Academic Institutions and Nonprofits only

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pJC6-GL3

Plasmid

#11979

Purpose

Reporter gene with mouse c-jun promoter.

Depositor

Ron Prywes

Article

Clarke et al Mol Cell Biol. 1998 Feb . 18(2):1065-73.

Insert

c-jun promoter (Jun Mouse)

Use

Luciferase

Tags

Luc

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
AAV-ReaChR-citrine

Plasmid

#50954

Purpose

ReaChR-citrine in AAV2 vector under human synapsin promoter

Depositor

Roger Tsien

Article

Lin et al Nat Neurosci. 2013 Oct;16(10):1499-508.

Insert

ReaChR-citrine

Use

AAV

Tags

citrine

Promoter

truncated human Synapsin promoter

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-E

Plasmid

#78852

Purpose

Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which contains eSpCas9 and puromycin cassette

Depositor

Phillip Abbosh

Article

Sikder et al Mol Cancer Res. 2020 Dec 3. pii: 1541-77

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Cas9-P2A-Puro

Expression

Mammalian

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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AAV CMV-dSaCas9-KRAB-bGHpA

Plasmid

#106219

Purpose

AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

Depositor

Charles Gersbach

Article

Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

Insert

dead S. aureus Cas9 KRAB

Use

AAV and CRISPR

Tags

HA-NLS

Expression

Mammalian

Mutation

D10A and N580A

Promoter

CMV

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pC019 - LwCas13a from Leptotrichia wadei

Plasmid

#91909

Purpose

Expresses LwCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LwCas13a and guide expression cassette

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 4, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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PGC-1 alpha promoter luciferase delta CRE

Plasmid

#8888

Depositor

Bruce Spiegelman

Article

Handschin et al Proc Natl Acad Sci U S A. 2003 Jun 10. 1

Insert

PGC-1 alpha promoter dCRE (Ppargc1a Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Site-directed mutagenesis to remove the CREB bind…

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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