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We narrowed to 3,144 results for: PPL;

Showing: 2721 - 2740 of 3144 results

  1. T13m

    Plasmid
    #121032
    Purpose
    MoClo golden gate assembly DE part for ECK120033737 (313x-strength natural terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ECK120033737 (second strongest natural Voigt terminator)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121032-plasmid-map-sequence-id-236342

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  2. C100m

    Plasmid
    #121004
    Purpose
    MoClo golden gate assembly CD part sfYFP-ptd3 (superfolder YFP with pdt3 degradation tag for mfLon). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    sfYFP with pdt3 tag
    Use
    Synthetic Biology
    Tags
    pdt3 mfLon degradation tag
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121004-plasmid-map-sequence-id-236143

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  3. C41m

    Plasmid
    #120974
    Purpose
    MoClo golden gate assembly CD part for dCas9 (Catalytically-inactive S. pyogenes Cas9, for gRNA-targeted repression). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    dCas9
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120974-plasmid-map-sequence-id-236028

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  4. C61m

    Plasmid
    #120977
    Purpose
    MoClo golden gate assembly CD part for RpaR* (improved RpaR quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    RpaR from Scott and Hasty 2016
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120977-plasmid-map-sequence-id-236031

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  5. C62m

    Plasmid
    #120978
    Purpose
    MoClo golden gate assembly CD part for TraR(W) (improved TraR quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    TraR(W) from Scott and Hasty 2016
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120978-plasmid-map-sequence-id-236032

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  6. P12m

    Plasmid
    #120938
    Purpose
    MoClo golden gate assembly AB part for pLas-TetO (Promoter activated by LasR and repressed by TetR; encodes <3OC12 AHL AND aTc> logic). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ohAB carb pLasTetO
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120938-plasmid-map-sequence-id-256615

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  7. P15m

    Plasmid
    #120939
    Purpose
    MoClo golden gate assembly AB part for pCin-TetO (Promoter activated by CinR and repressed by TetR; encodes <3OC14 AHL AND aTc> logic). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ohAB carb pCinTetO
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120939-plasmid-map-sequence-id-238035

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  8. P16m

    Plasmid
    #120940
    Purpose
    MoClo golden gate assembly AB part for pCin-LacO (Promoter activated by CinR and repressed by LacI; encodes <3OC14 AHL AND IPTG> logic). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ohAB carb pCinLacO m
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120940-plasmid-map-sequence-id-238036

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  9. P32m

    Plasmid
    #120943
    Purpose
    MoClo golden gate assembly AB part for PlacO (common LacI-repressed promoter; see DOI: 10.1126/science.1256272). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    PlacO (Tim Lu, Matt Bennett, etc)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120943-plasmid-map-sequence-id-236887

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  10. P33m

    Plasmid
    #120944
    Purpose
    MoClo golden gate assembly AB part (medium-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    P11 from Mutalik library (medium)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120944-plasmid-map-sequence-id-245421

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  11. P34m

    Plasmid
    #120945
    Purpose
    MoClo golden gate assembly AB part (low-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    P13 from Mutalik library (weak)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120945-plasmid-map-sequence-id-238039

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  12. pOpen-BstpolLF

    Plasmid
    #165553
    Purpose
    Fragment retains 5'-3' polymerase activity from full length Bst DNA Polymerase, while lacking 5'-3' exonuclease activity. Suitable for applications requiring thermophilic strand displacement.
    Depositor
    Jennifer Molloy, Drew Endy, FreeGenes Project
    Insert
    Bst DNA Polymerase, Large Fragment
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165553-plasmid-map-sequence-id-339898

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  13. C39m

    Plasmid
    #120972
    Purpose
    MoClo golden gate assembly CD part for ccdB (DNA gyrase toxin, modified synonymously to remove BsaI cut site; ccdA/C79m is antitoxin). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ccdB toxin
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI site removed
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120972-plasmid-map-sequence-id-236024

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  14. BII-gR-PnTW

    Plasmid
    #133356
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS
    Depositor
    Blair Madison
    Insert
    tdTomato-NLS
    Use
    Tags
    HA-tagged tdTomato
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available Since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133356-plasmid-map-sequence-id-267393

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  15. C67m

    Plasmid
    #120981
    Purpose
    MoClo golden gate assembly CD part for Bxb1-mScarleti-LAA (Bxb1 integrase tagged with an mScarlet-I reporter and an LAA degradation tag). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Bxb1 integrase - mScarlet-i - LAA tag
    Use
    Synthetic Biology
    Tags
    ssrA degradation tag
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120981-plasmid-map-sequence-id-236036

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  16. UC16m

    Plasmid
    #121038
    Purpose
    MoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    gQi Cas9 gRNA UC part
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121038-plasmid-map-sequence-id-236383

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  17. UC17m

    Plasmid
    #121039
    Purpose
    MoClo golden gate assembly DE part for gV1 gRNA (guide RNA for S. pyogenes Cas9; sequence from doi: [10.15252/msb.20145735]). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    gV1 Cas9 gRNA UC part
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121039-plasmid-map-sequence-id-236352

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  18. C105m

    Plasmid
    #121008
    Purpose
    MoClo golden gate assembly CD part for sfYFP-AAV (superfolder YFP with AAV degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    sfYFP-AAV ssrA (has bbsI)
    Use
    Synthetic Biology
    Tags
    AAV degradation tag for ssrA
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121008-plasmid-map-sequence-id-236163

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  19. C106m

    Plasmid
    #121009
    Purpose
    MoClo golden gate assembly CD part for sfYFP-DAS (superfolder YFP with DAS degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    sfYFP-DAS ssrA (has bbsI)
    Use
    Synthetic Biology
    Tags
    DAS degradation tag for ssrA
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121009-plasmid-map-sequence-id-236172

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  20. C109m

    Plasmid
    #121011
    Purpose
    MoClo golden gate assembly CD part for mfLon-pdt3 (mfLon protease with self-targeting degradation tag pdt3). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    mfLon protease with pdt3 tag
    Use
    Synthetic Biology
    Tags
    pdt3 mfLon degradation tag
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121011-plasmid-map-sequence-id-236323

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Showing: 2721 - 2740 of 3144 results