We narrowed to 22,436 results for: Sele

1. pNeae2

   Plasmid

   #168300

   Purpose

   Bacterial surface display of polypeptides (nanobodies) in E. coli

   Depositor

   Luis Ángel Fernández

   Article

   Salema et al PLoS One. 2013 Sep 23;8(9):e75126. doi:

   Insert

   Enterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus
   

   Use

   Tags

   6xHis, E-tag, and myc-tag

   Expression

   Bacterial

   Mutation

   Promoter

   LacI-Plac

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAC150-RFP-EGFP-LGALS3

   Plasmid

   #175778

   Purpose

   PiggyBac Vector expressing GFP-RFP-LGALS3

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   LGALS3 (LGALS3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hADM-RFP

   Plasmid

   #22922

   Depositor

   Edward Callaway

   Article

   Nathanson et al Front Neural Circuits. 2009 . 3():19.

   Insert

   human adrenomedullin promoter driving DsRedExpress (ADM Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEYFP-N1-Pink1

   Plasmid

   #101874

   Purpose

   Transient expression of Pink1-YFP in mammalian cells

   Depositor

   Richard Youle

   Article

   Narendra et al PLoS Biol. 2010 Jan 26;8(1):e1000298. do

   Insert

   PTEN induced putative kinase 1 (PINK1 Human)
   

   Use

   Tags

   fluorescent tag EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. PET-B-evoSpCas9-NLS-6xHis

   Plasmid

   #207390

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-evoSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M495V, Y515N, K526E, R661Q, amino acids 1005-1013…

   Promoter

   T7

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCWori cytochrome P450-BM3h 9D7

   Plasmid

   #61308

   Purpose

   Expresses Cytochrome P450-BM3h 9D7 with C-terminal 6-His tag

   Depositor

   Alan Jasanoff

   Article

   Brustad et al J Mol Biol. 2012 Sep 14;422(2):245-62. d

   Insert

   Cytochrome P450-BM3 heme domain 9D7
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   T268A, I263A, T438V, A328G

   Promoter

   Tac

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. p6XHis_NLS-SaCas9

   Plasmid

   #101086

   Purpose

   Express SaCas9 in bacteria with a 6xHis tag for purification

   Depositor

   Rick Tarleton

   Article

   Soares Medeiros et al MBio. 2017 Nov 7;8(6). pii: e01788-17. d

   Insert

   CRISPR-associated protein Cas9/Csn1
   

   Use

   CRISPR

   Tags

   TrxA-6xHis-STag

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. HA-GABARAPL1

   Plasmid

   #137759

   Purpose

   Expression of GABARAPL1

   Depositor

   Carol Mercer

   Article

   Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

   Insert

   GABARAPL1 (GABARAPL1 Human)
   

   Use

   Tags

   HA and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA-GABARAPL2

   Plasmid

   #137761

   Purpose

   Expression of GABARAPL2

   Depositor

   Carol Mercer

   Article

   Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

   Insert

   GABARAPL2 (GABARAPL2 Human)
   

   Use

   Tags

   HA and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-MAP1LC3A

    Plasmid

    #137756

    Purpose

    Expression of LC3A

    Depositor

    Carol Mercer

    Article

    Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

    Insert

    MAP1LC3A (MAP1LC3A Human)
    

    Use

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMaRSC

    Plasmid

    #89189

    Purpose

    The pMaRS plasmid is the same construct as pMaRSL274G, but contains a T2A-Mcherry sequence appended to the C-terminal of L274GMmMetRS.

    Depositor

    David Tirrell

    Article

    Mahdavi et al J Am Chem Soc. 2016 Apr 6;138(13):4278-8

    Insert

    Flag-L274G-T2A-mCherry (Mars1 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRDB_053

    Plasmid

    #216083

    Purpose

    for transgenic flies; gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31, destination vector

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31
    

    Use

    Destination

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUCmini-iCAP-PHP.B

    Plasmid

    #103002

    Purpose

    non-standard AAV2 rep-AAV-PHP.B cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

    Insert

    Synthetic construct isolate AAV-PHP.B VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti_Cas9_T2A_mNeonGreen_P2A_blasticidin

    Plasmid

    #211471

    Purpose

    lentiviral tricistronic complex expressing Cas9, mNeonGreen, and blasticidin resistance

    Depositor

    James Eshleman

    Article

    Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

    Insert

    Cas9_T2A_mNeonGreen_P2A_BlasticidinR
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, EF-1a

    Available since

    March 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. BB3cK_pGAP_23*_pTEF_Cas9

    Plasmid

    #104909

    Purpose

    hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLX304-IRES-Td

    Plasmid

    #176296

    Purpose

    pLX304 backbone with an IRES-Tdtomato cassette subcloned into the NheI-AgeI sites

    Depositor

    William Kaelin

    Article

    Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAJ075_hsCRBN

    Plasmid

    #124214

    Purpose

    Insect cell expression vector for His6 tagged hsCRBN

    Depositor

    Eric Fischer

    Article

    Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

    Insert

    Cereblon (CRBN Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-FLAG-APEX2-LGALS3

    Plasmid

    #175756

    Purpose

    Lentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS3

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    LGALS3 (LGALS3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLX304-IRES-GFP

    Plasmid

    #176288

    Purpose

    pLX304 backbone with an IRES-GFP cassette subcloned into the NheI-AgeI sites

    Depositor

    William Kaelin

    Article

    Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCEP4-myc-HLA-A24

    Plasmid

    #135507

    Purpose

    Mammalian expression of myc-tagged HLA-A*024:02

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*24:02 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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