We narrowed to 2,819 results for: aen

1. pdCas9-M-1E4

   Plasmid

   #73436

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1E4.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor 1E4 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available since

   March 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pdCas9-M-5F5

   Plasmid

   #73432

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 5F5.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor 5F5 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETM6-G6-vioABE-4A6-vioC-3A2-vioD

   Plasmid

   #73440

   Purpose

   Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   PG6-vioABE-P4A6-vioC-P3A2-vioD
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PG6, P4A6, and P3A2 (orthogonal T7-lac variants)

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19_mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP

   Plasmid

   #195505

   Purpose

   T-REX17 donor plasmid to generate locus specific integration of mRuby followed by 3xSV40 poly(A) into Exon 1 of human lncRNA T-REX17

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   pT-REX-mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP_T-REX(Ex1)
   

   Use

   CRISPR; Donor vector

   Tags

   mRuby-3xFLAG-NLS-3xSV40-poly(A)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19_T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP

   Plasmid

   #195503

   Purpose

   SOX17 donor plasmid to generate locus specific C-terminal integration of H2B-mCitrine into the human SOX17-locus

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   SOX17(Ex2)-T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP_SOX17(3'UTR) (SOX17 Human)
   

   Use

   CRISPR; Donor vector

   Tags

   T2A-H2B-mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST-Tol2-PA2-CMV-AB-mCh

   Plasmid

   #160435

   Purpose

   Expresses human Amyloid Beta-mCherry in Zebrafish

   Depositor

   Nicholas Tolwinski

   Article

   Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

   Insert

   Human Amyloid Beta peptide (1-42) (APP Human, Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. str-KDEL_Stx5LdeltaER-SBP-mCitrine

   Plasmid

   #154846

   Purpose

   Synchronize trafficking of Stx5LdeltaER (M55V) from the ER, for use with FLIM (RUSH system)

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   Stx5 (Long isoform) (STX5 Human)
   

   Use

   Tags

   Streptavidin Binding Protein (SBP) and mCitrine

   Expression

   Mammalian

   Mutation

   Changed Methionine 55 to Valine, causes loss of S…

   Promoter

   CMV

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRetro-CAG-DsRedExpress-G-TVA

   Plasmid

   #235698

   Purpose

   Expresses G protein, TVA receptor, and DsRed for rabies virus trans-synaptic tracing

   Depositor

   Hongjun Song, Guo-li Ming

   Article

   Sun et al Nature. 2025 Jan 16. doi: 10.1038/s41586

   Insert

   DsRedExpress, TVA800, and Glyco
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDV21 [pSNG-1::SNG-1::CRY2(D387A)olig(535)::SL2::mCherry]

   Plasmid

   #197599

   Purpose

   Pan-neuronal expression of SNG-1::CRY2(D387A)olig(535) in neurons of C. elegans. CRY2(D387A) is photoinactive

   Depositor

   Alexander Gottschalk

   Article

   Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:

   Insert

   Synaptogyrin-CRY2 (D387A+E490G + residues 1-535) (CRY2, sng-1 Nematode, Mustard Weed)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   D387A, E490G

   Promoter

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. str-KDEL_Stx5L(M55V)-SBP-mCitrine

    Plasmid

    #209846

    Purpose

    Synchronize trafficking of Stx5L (M55V) from the ER, for use with FLIM (RUSH system)

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    STX5 (STX5 Human)
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCitrine

    Expression

    Mammalian

    Mutation

    Changed Methionine 55 to Valine, causes loss of S…

    Promoter

    CMV

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX335_U6-Chimeric_BB-CBh-hSpCas9n(D10A)_SOX17_Ex2_KI

    Plasmid

    #195502

    Purpose

    Cas9 nickase expression vector bearing a sgRNA targeting Exon 2 of human SOX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    sgRNA (SOX17 Human)
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. mGFP-hNET-A457P

    Plasmid

    #193365

    Purpose

    expression of mutated human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cells

    Depositor

    Harald Sitte

    Article

    Luethi et al Commun Biol. 2022 Nov 17;5(1):1259. doi:

    Insert

    human Norepinephrine Transporter (A457P mutation) (SLC6A2 Human)
    

    Use

    Tags

    monomeric GFP (mGFP)

    Expression

    Mammalian

    Mutation

    A457P (GCC to CCC)

    Promoter

    CMV

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. mGFP-hNET-R121A/K334A/R440A

    Plasmid

    #193366

    Purpose

    expression of mutated human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cells

    Depositor

    Harald Sitte

    Article

    Luethi et al Commun Biol. 2022 Nov 17;5(1):1259. doi:

    Insert

    human Norepinephrine Transporter (SLC6A2 Human)
    

    Use

    Tags

    monomeric GFP (mGFP)

    Expression

    Mammalian

    Mutation

    R121A (CGG to GCG) K334A (AAA to GCA) R440A (CGA …

    Promoter

    CMV

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGFP-PGL-1-mHP1alpha

    Plasmid

    #181899

    Purpose

    Fluorescently tagged HP1alpha fused to PGL-1

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PGL-1-mHP1alpha (Cbx5 Mouse, Nematode)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRGG-GFP-RGG-mHP1alpha

    Plasmid

    #181898

    Purpose

    Fluorescently tagged HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    RGG-GFP-RGG (Cbx5 Mouse, Nematode)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. str-KDEL_Stx5S-SBP-mCitrine

    Plasmid

    #154845

    Purpose

    Synchronize trafficking of Stx5S from the ER, for use with FLIM (RUSH system)

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    Stx5 (Short isoform) (STX5 Human)
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCitrine

    Expression

    Mammalian

    Mutation

    Lacks amino acids 1-54, enocdes short isoform of …

    Promoter

    CMV

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLV-SFFV-PIK3CA-WPRE-UbC-Emerald

    Plasmid

    #203797

    Purpose

    Lentiviral vector expressing human phosphoinositide 3-kinase catalytic subunit alpha (PIK3CA) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Article

    Nieuwenhuis et al EMBO Mol Med. 2020 Aug 7;12(8):e11674. d

    Inserts

    PIK3CA (PIK3CA Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV-SFFV-PIK3CA H1047R-WPRE-UbC-Emerald

    Plasmid

    #203798

    Purpose

    Lentiviral vector expressing human PIK3CA H1047R under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Article

    Nieuwenhuis et al EMBO Mol Med. 2020 Aug 7;12(8):e11674. d

    Inserts

    PIK3CA (PIK3CA Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H1047R

    Promoter

    Available since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605

    Plasmid

    #108362

    Purpose

    Expression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegans

    Depositor

    Luisa Cochella

    Article

    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

    Inserts

    Ath-HEN1 (HEN1 Codon optimised for C. elegans, Mustard Weed)
    mCherry
    his-15 (his-15 Nematode)
    unc-54 3' UTR
    

    Use

    Tags

    2xFlag, MYC, T2A peptide, and TY1 linker

    Expression

    Worm

    Mutation

    Promoter

    A rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…

    Available since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. piggyBac-rtTA (4th_Gen)-NFIB-SOX9-IRES-mKate2 (VK_1244)

    Plasmid

    #209082

    Purpose

    NFIB and SOX9 mediated astrocye induction

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    NFIB-P2A-SOX9-V5 (NFIB, SOX9 Human)
    mKate2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE4G

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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