We narrowed to 17,455 results for: emb

1. NET5s pEGFP-N2 (1154)

   Plasmid

   #62034

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

   Insert

   NET5s (TMEM201 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   short form

   Promoter

   CMV

   Available Since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

2. NET5L pEGFP-N2 (1155)

   Plasmid

   #62035

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

   Insert

   NET5L (TMEM201 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   long form

   Promoter

   CMV

   Available Since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

3. TPC2-G-GECO1.2

   Plasmid

   #207141

   Purpose

   Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel

   Depositor

   Antony Galione

   Article

   Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

   Insert

   TPC2N2 (TPCN2 Human)
   

   Tags

   G-GECO1.2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. PP_070_pAAV_CAG_LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2

   Plasmid

   #203228

   Purpose

   Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

   Depositor

   Adam Cohen

   Insert

   Membrane-localized optopatch for neuronal dendrites
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG dCas9-KRAB-2A-EGFP

   Plasmid

   #92396

   Purpose

   CAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   dCas9_KRAB_2A_EGFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMV_AA050

   Plasmid

   #216107

   Purpose

   gDNA expression for Cas9 with ChenF+E trRNA (guide only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   gDNA expression for Cas9 with ChenF+E trRNA
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB533_2E12LI-sfGFP

   Plasmid

   #167527

   Purpose

   H3K27me3-mintbody expression vector for mammalian cells

   Depositor

   Hiroshi Kimura

   Article

   Tjalsma et al EMBO Rep. 2021 Feb 19:e51989. doi: 10.15

   Insert

   2E12LI-scFv
   

   Tags

   sfGFP

   Expression

   Mammalian

   Available Since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRDB_182

   Plasmid

   #216103

   Purpose

   CRISPRi, EF1a-driven dCas9-KRAB (ZIM3) (Cas only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   Cas9 [Sp]
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAd/CMV/V5-Clasp2α

   Plasmid

   #89763

   Purpose

   Adenovirus destination vector for transient expression of human wild-type EGFP-CLASP2α

   Depositor

   Torsten Wittmann

   Article

   Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

   Insert

   CLASP2α (CLASP2 Human)
   

   Use

   Adenoviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRDB_054

    Plasmid

    #216096

    Purpose

    All-in-one, knockout, delivers Cas12a & gRNA

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas12a [EnAs]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSP1

    Plasmid

    #20060

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1 (APOA1 synthetic gene)
    

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    synthetic gene of deletion mutant (1-43) of human…

    Available Since

    Jan. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS-PKCdelta-EGFP

    Plasmid

    #178856

    Purpose

    Mammalian expression of PKCδ fused to EGFP

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    PKCδ (PRKCD Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Stargazin-mCherry-FRB

    Plasmid

    #172443

    Purpose

    Expression of plasma membrane protein Stargazin tagged with mCherry-FRB

    Depositor

    Stephen Royle

    Article

    Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    Cacng2 (Cacng2 Mouse)
    

    Tags

    mCherry-HA-FRB

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-STAT2

    Plasmid

    #71451

    Purpose

    3rd generation lentiviral expression of STAT2

    Depositor

    George Stark

    Article

    Cheon et al EMBO J. 2013 Oct 16;32(20):2751-63. doi:

    Insert

    STAT2 (STAT2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Promoter

    CMV

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.U6gT28.4.hSyn.H2B.RFP

    Plasmid

    #170387

    Purpose

    Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Expressing nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    H2B RFP
    

    Use

    AAV

    Promoter

    human Synapsin

    Available Since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. siRNA-resistant ss-SigmaR1DTM-mNeonGreen R119A F191A

    Plasmid

    #226576

    Purpose

    Encodes mutant siRNA resistant SigmaR1 protein (transmembrane region deletion + substitutions) labeled with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    R119A, F191A

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330a dCas9-LSD1

    Plasmid

    #92362

    Purpose

    Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to LSD1. For targeted enhancer demethylation in chicken embryos.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    dCas9-LSD1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETM-41-HsPABPC1_J

    Plasmid

    #146644

    Purpose

    Bacterial Expression of HsPABPc1

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

    Insert

    HsPABPc1 (PABPC1 Human)
    

    Expression

    Bacterial

    Mutation

    two silent mutations compared to the sequence giv…

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330a dCas9-KRAB

    Plasmid

    #92361

    Purpose

    Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. For targeted enhancer inactivation in chicken embryos.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    dCas9-KRAB
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. Tom70 (1-70)-GFP

    Plasmid

    #127633

    Purpose

    Mitochondrial targeting sequence of Tom70 fused to GFP

    Depositor

    Josef Kittler

    Article

    Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

    Insert

    Tom70 (TOMM70 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Only first 70 amino acids

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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