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We narrowed to 10,323 results for: Coli

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Showing: 5481 - 5520 of 10323 results

  1. pLY59

    Plasmid
    #130927
    Purpose
    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::MCP controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    sfgfp
    pspFΔHTH::MCP
    Use
    Synthetic Biology
    Tags
    ASV tag and MCP (MS2 coat protein)
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Anderson promoter: J23106, PpspA-2G6, and Ptet
    Available Since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    130927-plasmid-map-sequence-id-257328

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  2. pLY57

    Plasmid
    #130926
    Purpose
    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA5B5 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Anderson promoter: J23106, PpspA-LEA5B5, and Ptet
    Available Since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    130926-plasmid-map-sequence-id-257327

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  3. pLY56

    Plasmid
    #130925
    Purpose
    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA4B4 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Anderson promoter: J23106, PpspA-LEA4B4, and Ptet
    Available Since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    130925-plasmid-map-sequence-id-257326

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  4. pLY55

    Plasmid
    #130924
    Purpose
    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Anderson promoter: J23106, PpspA-LEA3B3, and Ptet
    Available Since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    130924-plasmid-map-sequence-id-257309

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  5. pLY53

    Plasmid
    #130911
    Purpose
    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Anderson promoter: J23106, PpspA-LEA1B1, and Ptet
    Available Since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    130911-plasmid-map-sequence-id-257304

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  6. pdCas9-M-4F2

    Plasmid
    #73431
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4F2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 4F2 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available Since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73431-plasmid-map-sequence-id-135331

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  7. pdCas9-M-1B6

    Plasmid
    #73430
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1B6.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 1B6 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available Since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73430-plasmid-map-sequence-id-139104

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  8. pdCas9-M-3H5

    Plasmid
    #73429
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3H5.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 3H5 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available Since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73429-plasmid-map-sequence-id-135329

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  9. pdCas9-M-1D4

    Plasmid
    #73433
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1D4.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 1D4 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73433-plasmid-map-sequence-id-135333

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  10. pdCas9-M-1E4

    Plasmid
    #73436
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1E4.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 1E4 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available Since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    73436-plasmid-map-sequence-id-135335

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  11. pdCas9-M-5F5

    Plasmid
    #73432
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 5F5.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 5F5 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73432-plasmid-map-sequence-id-135332

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  12. pChillyBoys_gen1

    Plasmid
    #228495
    Purpose
    pACYCDuet-1 encoding Oleispira antarctica GroEL and GorES low temp chaperonins for enhanced low-temperature induction (E. coli based protein expression)
    Depositor
    Richard Baker
    Inserts
    groEL
    groES
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    228495-plasmid-map-sequence-id-454882

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  13. SUMO-PNGaseF

    Plasmid
    #228498
    Purpose
    10xHis-SUMO (Smt3) tagged PNGaseF (E. coli based protein expression)
    Depositor
    Richard Baker
    Insert
    ngl
    Tags
    10xHis-SUMO(Smt3)
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    228498-plasmid-map-sequence-id-455750

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  14. pTCF158 Human beta-2 microglobulin

    Plasmid
    #180457
    Purpose
    E. coli expression of class I MHC light chain
    Depositor
    John Altman
    Insert
    Human beta-2 microglobulin
    Expression
    Bacterial
    Available Since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180457-plasmid-map-sequence-id-355801

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  15. pET28-H12TEV

    Plasmid
    #208635
    Purpose
    for E. coli expression of large protein, insert 6xhis + TEV site using NdeI and BamHI site in pET28a(+), note that it is 6xHis-Thrombin-6xHis-TEV, not continuous 12xHis. BamHI in-frame with tag.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    6xhis
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208635-plasmid-map-sequence-id-433640

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  16. pAIP4_3KB5

    Plasmid
    #234225
    Purpose
    Heterologous protein expression of tripartite motif-containing protein 72 (Mg53) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3KB5 (TRIM72 Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234225-plasmid-map-sequence-id-464190

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  17. pET28a_PTK2b

    Plasmid
    #224237
    Purpose
    Expression of human PTK2b Kinase in E. coli
    Depositor
    Andrew Mesecar
    Insert
    ptk2b (PTK2B Human)
    Tags
    6xHis and 6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7 promotor
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    224237-plasmid-map-sequence-id-458420

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  18. pET11a_PTK2b_Kinase

    Plasmid
    #224242
    Purpose
    Expression of human PTK2b Kinase domain in E. coli
    Depositor
    Andrew Mesecar
    Insert
    ptk2b_kinase (PTK2B Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7 promotor
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    224242-plasmid-map-sequence-id-458378

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  19. pet15b_ack

    Plasmid
    #220229
    Purpose
    Bacterial expression of AcK in E. coli BL21_DE3 (pLys) or similar
    Depositor
    Bernd Nidetzky
    Insert
    Acetate kinase (ackA )
    Tags
    Histag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    220229-plasmid-map-sequence-id-436823

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  20. ScSpe1_pET20b(+)

    Plasmid
    #118343
    Purpose
    Expresses the His tagged Ornithine Decarboxylase from Saccharomyces cerevisiae in Escherichia coli BL21 or similar strains
    Depositor
    Markus Ralser
    Insert
    SPE1
    Tags
    Histidine
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    118343-plasmid-map-sequence-id-229413

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  21. pBS3ndd

    Plasmid
    #97354
    Purpose
    Low background cloning vector for conventional or homologous recombination cloning with sticky blunt or TA compatible ends, kanamycin resistant
    Depositor
    Jamorn Somana
    Insert
    Nucleoid Disruptive Domain
    Use
    Cloning
    Expression
    Bacterial
    Mutation
    changed methionine 1 to isoleucine
    Promoter
    E. coli lacZ promoter
    Available Since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    97354-plasmid-map-sequence-id-191848

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  22. pE-SUMO CRYGB

    Plasmid
    #80751
    Purpose
    Expression of human gamma B-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYGB (CRYGB Human)
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80751-plasmid-map-sequence-id-153519

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  23. pE-SUMO CRYGC

    Plasmid
    #80754
    Purpose
    Expression of human gamma C-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYGC (CRYGC Human)
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80754-plasmid-map-sequence-id-153567

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  24. MtDalaDala-pNIC-Bsa4

    Plasmid
    #250149
    Purpose
    Expresses Mycobacterium tuberculosis D-alanine D-alanine under T7 with HIS tag in bacterial cells
    Depositor
    Josh Beckham
    Insert
    D-alanine D-alanine
    Tags
    6x His Tag
    Expression
    Bacterial
    Mutation
    Codon optimized E. coli
    Available Since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250149-plasmid-map-sequence-id-499143

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  26. Sc FLAG-S6-RFC1-pRSFDuet

    Plasmid
    #239472
    Purpose
    Overexpress Sc FLAG-S6-RFC1 in E. coli
    Depositor
    Michael O'Donnell
    Insert
    Sc FLAG-S6-RFC1 (RFC1 Budding Yeast)
    Tags
    3X FLAG followed by S6
    Expression
    Bacterial
    Available Since
    Aug. 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    239472-plasmid-map-sequence-id-481685

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  27. pAIP4_4J5Q

    Plasmid
    #234233
    Purpose
    Heterologous protein expression of ADP-ribose glycohydrolase OARD1 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4J5Q (tas3 Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234233-plasmid-map-sequence-id-464197

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  28. pAIP4_3OBS

    Plasmid
    #234227
    Purpose
    Heterologous protein expression of tumor susceptibility gene 101 protein in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3OBS (TSG101 Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234227-plasmid-map-sequence-id-464192

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  29. pAIP4_5GUQ

    Plasmid
    #234171
    Purpose
    Heterologous protein expression of ASCH domain-containing ribonuclease in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5GUQ
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234171-plasmid-map-sequence-id-464137

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  30. pAIP4_3V46

    Plasmid
    #234165
    Purpose
    Heterologous protein expression of cell division control protein 73 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3V46 (CDC73 Budding Yeast)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234165-plasmid-map-sequence-id-464131

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  31. pAIP4_5IHW

    Plasmid
    #234148
    Purpose
    Heterologous protein expression of serine-aspartate repeat-containing protein E in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5IHW
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234148-plasmid-map-sequence-id-464114

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  32. pAIP4_2OKT

    Plasmid
    #234149
    Purpose
    Heterologous protein expression of o-succinylbenzoate synthase (OSB synthase) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2OKT
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234149-plasmid-map-sequence-id-464115

    Enlarge

  33. pBS2ndd

    Plasmid
    #97353
    Purpose
    Low background cloning vector for conventional or homologous recombination cloning with sticky blunt or TA compatible ends, ampicillin resistant
    Depositor
    Jamorn Somana
    Insert
    Nucleoid Disruptive Domain
    Use
    Cloning
    Expression
    Bacterial
    Mutation
    changed methionine 1 to isoleucine
    Promoter
    E. coli lacZ promoter
    Available Since
    Aug. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    97353-plasmid-map-sequence-id-190845

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  34. pHL 1915

    Plasmid
    #53022
    Purpose
    AmpR + PLlacO1fims::gfp::Asp terminator + CamR + ColE1 (PLlacO1fimS is a fused PLlacO-1 and fimS promoter)
    Depositor
    Han Lim
    Insert
    GFP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    pLlacO-1 and fimS fusion
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  35. pE-SUMO CRYGD

    Plasmid
    #80755
    Purpose
    Expression of human gamma D-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYGD (CRYGD Human)
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80755-plasmid-map-sequence-id-153570

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  36. pTCF156 HLA-A*02:01

    Plasmid
    #180456
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-A*02:01
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available Since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180456-plasmid-map-sequence-id-355799

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  37. pJA5 H-2K(b)

    Plasmid
    #180441
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    H-2K(b)
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    180441-plasmid-map-sequence-id-355754

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  38. pJA54 HLA-A*03:01

    Plasmid
    #180451
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-A*03:01
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    180451-plasmid-map-sequence-id-355758

    Enlarge

  39. pTCF200 Mouse beta-2 microglobulin

    Plasmid
    #180462
    Purpose
    E. coli expression of class I MHC light chain
    Depositor
    John Altman
    Insert
    Mouse beta-2 microglobulin
    Tags
    BSP41
    Expression
    Bacterial
    Available Since
    Feb. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    180462-plasmid-map-sequence-id-355083

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  40. pTCF120 H-2D(b)

    Plasmid
    #180454
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    H-2D(b)
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available Since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180454-plasmid-map-sequence-id-355842

    Enlarge

Showing: 5481 - 5520 of 10323 results