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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,695 results for: Pol

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Showing: 2741 - 2760 of 7695 results

  4. TEV Protease-mCherry

    Plasmid
    #243793
    Purpose
    Encodes for mammalian expression of TEV protease input. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.
    Depositor
    Cole DeForest
    Publication
    Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TEV protease
    Expression
    Mammalian
    Available since
    Oct. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    243793-plasmid-map-sequence-id-486452

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  9. pOCC65

    Plasmid
    #118865
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal monomeric GFP
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-mGFP-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease, mGFP
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118865-plasmid-map-sequence-id-232317

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  12. RPS6KA1

    Plasmid
    #39021
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RPS6KA1 (RPS6KA1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. pVSL10

    Plasmid
    #201182
    Purpose
    Bst polymerase large fragment (BF) F710Y/D598A, His6-tagged, HRV 3C-cleavable
    Depositor
    Jack Szostak
    Publication
    Lelyveld et al Science. 2023 Oct 27;382(6669):423-429.
    Insert
    Bst polymerase large fragment (BF) F710Y/D598A
    Tags
    HRV 3C protease cleavage site and His6
    Expression
    Bacterial
    Mutation
    F710Y D598A
    Promoter
    T5-lacO
    Available since
    Sept. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    201182-plasmid-map-sequence-id-406202

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  14. pOPINB-M1-di-Ub(G76V)-HOIL-1

    Plasmid
    #229539
    Purpose
    Constitutively active M1-di-ubiquitin HOIL-1 fusion protein for expression in E. coli
    Depositor
    Bernhard Lechtenberg
    Publication
    Wang et al Life Sci Alliance. 2025 Apr 1;8(6):e2025
    Inserts
    Ubiquitin (UBC Human)
    Ubiquitin (UBC Human)
    HOIL-1 (RBCK1 Human)
    Tags
    6xHis-3C
    Expression
    Bacterial
    Mutation
    Changed Gly 76 to Val and changed Gly 76 to Val
    Promoter
    T7
    Available since
    Dec. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    229539-plasmid-map-sequence-id-456666

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  15. pAAV-UbC-mCherry Rab5

    Plasmid
    #115239
    Purpose
    To express in mammalian cells the endocytic protein Rab5 fused to mCherry by transfection or by generating AAV virions.
    Depositor
    Pantelis Tsoulfas
    Insert
    Rab5a (RAB5A Homo sapiens, Human)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    Human UbC
    Available since
    Sept. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    115239-plasmid-map-sequence-id-222083

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  16. rat APOBEC1 E63A

    Plasmid
    #112861
    Purpose
    plasmid for expression of a catalytically inactive rat APOBEC1 mutant in mammalian cells
    Depositor
    Silvestro Conticello
    Publication
    Chieca et al bioRxiv 2021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    APOBEC1 (Apobec1 Rat)
    Expression
    Mammalian
    Mutation
    changed Glutamic acid 63 to Alanine
    Promoter
    CMV promoter
    Available since
    Aug. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    112861-plasmid-map-sequence-id-296442

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  17. pNIC28-Bsa4_ZV-NS5-RdRp

    Plasmid
    #179847
    Purpose
    The RdRp domain (with a 6-histidine tag) from Zika virus nonstructural protein 5 (French Polynesia strain, GenBank accession number ARU07075.1) encompassing residues 270 to 89
    Depositor
    Julien Lescar
    Publication
    Gharbi-Ayachi et al J Virol. 2020 Oct 14;94(21). pii: JVI.00
    Insert
    Zika Virus Non-structural protein 5 RdRp domain
    Tags
    6-histine
    Expression
    Bacterial
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    179847-plasmid-map-sequence-id-365329

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Showing: 2741 - 2760 of 7695 results