We narrowed to 29,768 results for: REP

1. Ssh3 gRNA#3

   Plasmid

   #163402

   Purpose

   Cas9-mediated knockout of Ssh3 in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Ssh3 (Ssh3 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAFNF-Cdc42

   Plasmid

   #163606

   Purpose

   Expresses Cdc42 Flp-dependently in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Cdc42 (Cdc42 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Ssh3 gRNA#1

   Plasmid

   #163400

   Purpose

   Cas9-mediated knockout of Ssh3 in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Ssh3 (Ssh3 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTub-alpha-1-mtGFP

   Plasmid

   #138981

   Purpose

   Neuron-specific expression of membrane targeted EGFP

   Depositor

   Sebastián Dupraz, Frank Bradke

   Article

   Santos et al Cell Rep. 2020 Jul 21;32(3):107907. doi:

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EGFP (added membrane targeting domain from GAP43).

   Promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET11a-Z-N-DiB2

   Plasmid

   #168473

   Purpose

   N-fragment of the DiB2‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain DiB2‐split-Zip protein

   Depositor

   Jens Meiler

   Article

   Bozhanova et al Sci Rep. 2020 Jul 6;10(1):11049. doi: 10

   Insert

   N-fragment of DiB2 + Leucine Zipper
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP_V577D-talin1 (1-2541)

   Plasmid

   #166115

   Purpose

   expresses talin-1-V577D in mammalian cells

   Depositor

   Vesa Hytönen

   Article

   Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

   Insert

   V577D-Talin-1 (Tln1 Mouse)
   

   Use

   Cmv promoter

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP_P229L-talin1 (1-2541)

   Plasmid

   #166113

   Purpose

   expresses talin-1-P229L in mammalian cells

   Depositor

   Vesa Hytönen

   Article

   Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

   Insert

   P229L-Talin-1 (Tln1 Mouse)
   

   Use

   Cmv promoter

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGMR-WhiteA2

   Plasmid

   #165874

   Purpose

   Basic transgenesis 5xGMR white CDS physical eye marker cassette in Alpha2, lacks attB site

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Eye Marker Cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJJF144_SECGFP_sg2

   Plasmid

   #163865

   Purpose

   sgRNA targeting GFP in C. elegans. Backbone: pJJR50.

   Depositor

   Nikolaus Rajewsky

   Article

   Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

   Insert

   sgRNA targeting GFP
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   R07E5.16 (U6)

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJJF152_Phsp_Cas9

    Plasmid

    #163862

    Purpose

    heat shock-induced expression of Cas9 in C. elegans

    Depositor

    Nikolaus Rajewsky

    Article

    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

    Insert

    Cas9, C. elegans codon optimized with intron and SV40_NLS
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Phsp-16.48

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJJF143_SECGFP_sg1

    Plasmid

    #163864

    Purpose

    sgRNA targeting GFP in C. elegans. Backbone: pJJR50.

    Depositor

    Nikolaus Rajewsky

    Article

    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

    Insert

    sgRNA targeting GFP
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    R07E5.16 (U6)

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPuroR-GMRWhite

    Plasmid

    #165884

    Purpose

    Puromycin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Transgenesis Ready Selection Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pISH-OMACS

    Plasmid

    #136765

    Purpose

    Template to synthesize riboprobes for in situ hybridization (ISH) for Acsm4

    Depositor

    Peter Mombaerts

    Article

    Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

    Insert

    Acsm4 (Acsm4 Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Mutation

    ISH Probe insert, A102T- please see depositor com…

    Promoter

    Available since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC_h7SK M15 (CUA)

    Plasmid

    #154784

    Purpose

    plasmid with single copy of Mma PylT M15 mutant (CUA anticodon)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    Mma PylT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G10A, G14U, G16U, C20U, C52A

    Promoter

    h7SK

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC_h7SK M15 (UUA)

    Plasmid

    #154785

    Purpose

    plasmid with single copy of Mma PylT M15 mutant (UUA anticodon)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    Mma PylT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    UUA anticodon and G10A, G14U, G16U, C20U, C52A

    Promoter

    h7SK

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pF3BGX-mTurquoise2

    Plasmid

    #138390

    Purpose

    RMCE vector for generating C-terminal mTurqouise2 fusion

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-4T-1-3xVSVG-GSK3(1-97)

    Plasmid

    #129582

    Purpose

    Encodes VSVG tagged GSK3(1-97) for purification by GST affinity

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Insert

    GSK3a(1-97) (GSK3A Human)
    

    Use

    Tags

    GST and VSVG

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-29b-3xVSVG-GSK3(1-97)

    Plasmid

    #129583

    Purpose

    Encodes VSVG tagged GSK3(1-97) for purification by nickel affinity

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Insert

    GSK3a(1-97) (GSK3A Human)
    

    Use

    Tags

    6xHistidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. kank1b_pJet1.2

    Plasmid

    #121979

    Purpose

    for generating kank1b riboprobes for WISH in zebrafish

    Depositor

    GuangJun Zhang

    Article

    Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

    Insert

    kank1b (si:dkey-226m8.9 Zebrafish)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Tol2-lyzC-RFP-miR-30 backbone

    Plasmid

    #97147

    Purpose

    As a control of miRNA expression

    Depositor

    Qing Deng

    Article

    Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

    Type

    Empty backbone

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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