We narrowed to 5,412 results for: U6

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pLenti_CHyMErA_U6(SpCas9_I-SceI)-(AsCas12_PacI)_PGK-puro

Plasmid

#189634

Purpose

Lentiviral expression of single SpCas9 and AsCas12a gRNAs for generating combinatorial CHyMErA 3Cs libraries

Depositor

Manuel Kaulich

Article

Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

Insert

hU6 Cas9-Cas12a gRNA cassette, PGK puro cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2

Plasmid

#189632

Purpose

Lentiviral expression of dual SpCas9 gRNAs for generating combinatorial SpCas9 3Cs libraries

Depositor

Manuel Kaulich

Article

Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

Insert

h7SK Cas9 gRNA cassette, hU6 Cas9 gRNA cassette, PGK puro cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-CMV-Cre-U6-sgNotch2#1-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

Plasmid

#209069

Purpose

Entry vector that encodes sgRNAs against mouse Notch2, Rb1, Trp53, Rbl2, and CMV Cre recombinase.

Depositor

Matthew Oser

Article

Hong et al Cancer Res. 2022 Jan 15;82(2):248-263. d

Insert

sgRNAs targeting Notch2, Rb1, Trp53, Rbl2 and Cre recombinase

Use

Gateway vector to be used for lr reaction

Promoter

U6

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-CMV-Cre-U6-sgNotch1#1-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

Plasmid

#209068

Purpose

Entry vector that encodes sgRNAs against mouse Notch1, Rb1, Trp53, Rbl2, and CMV Cre recombinase.

Depositor

Matthew Oser

Article

Hong et al Cancer Res. 2022 Jan 15;82(2):248-263. d

Insert

sgRNAs targeting Notch1, Rb1, Trp53, Rbl2 and Cre recombinase

Use

Gateway vector to be used for lr reaction

Promoter

U6

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pDest_U6_A2_CG2_T3_gRNA

Plasmid

#199389

Purpose

pDEL113; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type III sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

nrg1 type III sgRNA

Use

Tol2 destination vector

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pDest_U6_A2_CG2_erbb2_gRNA171

Plasmid

#199385

Purpose

pDEL133; Gateway Destination vector for cell-type specific CRISPR encoding erbb2 sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

erbb2 sgRNA 171

Use

Tol2 destination vector

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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PlayBack-U6-Bamh1

Plasmid

#203298

Purpose

For expression of sgRNAs in a PlayBack Vector with Bamh1 as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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PlayBack-U6-Bgl11

Plasmid

#203299

Purpose

For expression of sgRNAs in a PlayBack Vector with BglII as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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PlayBack-U6-Not1

Plasmid

#203301

Purpose

For expression of sgRNAs in a PlayBack Vector with NotI as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-DCK-Hygro

Plasmid

#202409

Purpose

gRNA expression vector for DCK

Depositor

Morito Kurata

Article

Sugita et al Commun Biol. 2023 Jun 1;6(1):582. doi: 1

Insert

guide RNA (DCK Human)

Use

CRISPR and Lentiviral

Tags

Dck

Promoter

U6

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEXfrt-SaCas9-U6-sgNtsr1(guide_2)

Plasmid

#205417

Purpose

Mutagenesis of Ntsr1, second guide

Depositor

Larry Zweifel

Insert

Ntsr1 (Ntsr1 Mouse)

Use

AAV and CRISPR

Promoter

U6

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-MP-84-spCas9-U6-gRNA-tlock

Plasmid

#206936

Purpose

Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.

Depositor

Markus Grompe

Insert

spCas9

Use

AAV

Tags

SV40 NLS

Expression

Mammalian

Promoter

MP-84

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-Bs3-sgRNA (BsaI)

Plasmid

#188280

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2019 Apr 23;5:23. doi: 10.1

Insert

Bs3-sgRNA

Use

CRISPR

Expression

Mammalian

Mutation

none

Promoter

U6

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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U6-TM2Emp-6T_iBlue-ModFlanks

Plasmid

#200236

Purpose

Backbone for cloning longer RNA triggers that activate iSBH-sgRNAs

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Type

Empty backbone

Expression

Mammalian

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDest_U6_A2_CG2_typeEGF_gRNA

Plasmid

#199386

Purpose

pDEL110; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 EGF sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

nrg1 EGF sgRNA

Use

Tol2 destination vector

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-sgRNA(OXTR.2)-CMV-eGFP

Plasmid

#194016

Purpose

Expresses a gRNA that targets OXTR coding sequence in multiple (rodent) species and eGFP

Depositor

Larry Young

Article

Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

Inserts

sgRNA(Oxtr.2)
eGFP

Use

AAV and CRISPR

Expression

Mammalian

Promoter

cmb and u6

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pRDA52_Lenti.puro.U6_modified DR

Plasmid

#196723

Purpose

For cloning pairs of AsCas12a guides with two DRs that are as different as possible.

Depositor

Bernhard Schmierer

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV2ss-U6-sgGFP

Plasmid

#190899

Purpose

AAV vector expressing sgGFP

Depositor

Nicole Déglon

Article

Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

Insert

sgRNA

Use

AAV and CRISPR

Promoter

U6

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAVss-U6-sgHTT51-7sk-Cas9

Plasmid

#190901

Purpose

AAV-KamiCas9 vector expressing sgGFP and mouse HTT sgRNA

Depositor

Nicole Déglon

Article

Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

Insert

sgRNA (Htt Mouse)

Use

AAV and CRISPR

Promoter

U6 and 7sk

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1

Plasmid

#190902

Purpose

AAV vector expressing thew GFP reporter gene and human sgHTT

Depositor

Nicole Déglon

Article

Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

Insert

GFP and sgHTT (HTT Human)

Use

AAV and CRISPR

Promoter

EFS and U6

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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