We narrowed to 3,171 results for: ALC

Showing: 2761 - 2780 of 3171 results

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Orai1-jGCaMP8f V203Y

Plasmid

#231631

Purpose

Green fluorescent reporter of Orai1-associated calcium influx

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2023 Sep 26;12

Insert

Orai1-jGCaMP8f V203Y (ORAI1 Synthetic, Human)

Tags

jGCaMP8f V203Y

Expression

Mammalian

Promoter

CMV Immediate Early

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-CIB1-Myc

Plasmid

#32505

Depositor

John Groffen, Nora Heisterkamp

Article

Haataja et al J Biol Chem. 2002 Mar 8;277(10):8321-8.

Insert

CIB1 (CIB1 Human)

Tags

Myc

Expression

Mammalian

Available Since

Jan. 26, 2012

Availability

Academic Institutions and Nonprofits only
pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8f-p10

Plasmid

#218866

Purpose

Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

Insert

RSET-jGCaMP8f

Tags

6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

Expression

Insect

Mutation

Q315L

Promoter

hsp70

Available Since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV2_hSyn_NES-Caprola_off-mEGFP_WRPE-SV40

Plasmid

#194692

Purpose

hSyn1 driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_off-mEGFP

Use

AAV

Tags

mEGFP

Promoter

hSyn1

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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ins:GCaMP6s; cryaa:RFP

Plasmid

#110285

Purpose

A zebrafish insulin promoter driving the calcium indicator, GCaMP6s. Contains red eye marker, and is flanked with I-SceI sites.

Depositor

Nikolay Ninov

Article

Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

Insert

GCaMP6s

Use

Zebrafish

Promoter

insulin

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-Venus-FLARE-Cameleon

Plasmid

#123351

Purpose

Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-Venus-FLARE-Cameleon

Tags

6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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CaMKIV-dCT

Plasmid

#126422

Purpose

Plasmid expresses constitutively active form of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)

Depositor

Douglas Black

Article

Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi

Insert

Calcium/calmodulin-dependent protein kinase type IV (CAMK4 Human)

Tags

FLAG

Expression

Mammalian

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8s-p10

Plasmid

#218864

Purpose

Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

Insert

RSET-jGCaMP8s

Tags

6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

Expression

Insect

Mutation

S26M F286Y Q315H

Promoter

hsp70

Available Since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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ER-CanlonicSF

Plasmid

#178343

Purpose

To explore endoplasmic reticulum lactate dynamics

Depositor

Alejandro San Martín

Article

Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

Insert

ER-CanlonicSF

Tags

ER Retention Signal, ER Signal Exon 1, and ER Sig…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP3-373

Plasmid

#91779

Purpose

Mammalian expression of low affinity calcium-sensing fluorescent protein

Depositor

Douglas Kim, GENIE Project

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

GCaMP3-373

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8f-p10

Plasmid

#218867

Purpose

LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

Insert

RSET-jGCaMP8f

Tags

6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

Expression

Insect

Mutation

Q315L

Promoter

hsp70

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pQTEV-MAP1LC3A

Plasmid

#34824

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

microtubule-associated protein 1 light chain 3 alpha (MAP1LC3A Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8s-p10

Plasmid

#218865

Purpose

LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

Insert

RSET-jGCaMP8s

Tags

6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

Expression

Insect

Mutation

S26M F286Y Q315H

Promoter

hsp70

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBEST-p70a-UTR1-NTerm 6xHis-zmADH-T500

Plasmid

#92228

Purpose

Expression of zmADH using the p70a promoter and UTR1

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

NTerm 6xHis Tagged zmADH

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Promoter

p70a

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-shNCLX-2

Plasmid

#181871

Purpose

Expresses NCLX-targeted shRNA under control of the U6 promoter and mCherry under control of the CaMKIIa promoter

Depositor

Hilmar Bading

Article

Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

Insert

solute carrier 8 family member B1 (Slc8b1 Mouse)

Use

AAV, Adenoviral, and Mouse Targeting

Tags

mCherry

Expression

Mammalian

Promoter

U6

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-shNCLX-1

Plasmid

#181870

Purpose

Expresses NCLX-targeted shRNA under control of the U6 promoter and mCherry under control of the CaMKIIa promoter

Depositor

Hilmar Bading

Article

Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

Insert

solute carrier 8 family member B1 (Slc8b1 Mouse)

Use

AAV, Adenoviral, and Mouse Targeting

Tags

mCherry

Expression

Mammalian

Promoter

U6

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3A no-stop

Plasmid

#123204

Purpose

Gateway entry clone encoding human MAP1LC3A lacking stop codon, suitable for C-terminal tagging

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)

Use

Gateway entry vector / entry clone

Mutation

Stop codon removed

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-mCherry-FLARE-Cameleon

Plasmid

#123352

Purpose

Red-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

mCherry-mCherry-FLARE-Cameleon

Tags

6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-LC3A G120A

Plasmid

#123090

Purpose

Expresses 3xFLAG-LC3A G120A in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Glycine 120 to Alanine

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pRSET a sfMatryoshCaMP6s-T78H

Plasmid

#100021

Purpose

Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78H

Depositor

Wolf Frommer

Article

Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

Insert

sfMatryoshCaMP6s

Tags

6x HIS tag and Xpress_EK tag

Expression

Bacterial

Mutation

cpEGFP replaced with superfolder cpGFP and T78H m…

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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