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Avs4_Chimera

Plasmid

#188802

Purpose

Chimeric Avs4 with transmembrane effector

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

Avs4 chimera

Expression

Bacterial

Promoter

EcAvs4 native promoter

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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His8OphMADeltaC6-18mer.

Plasmid

#189671

Purpose

His8 tagged OphMADeltaC6 with TEV cleavable C-terminus to generate truncated 18merTr and 15mer peptides

Depositor

Markus Künzler, James Naismith

Article

Emmanuel et al bioRxiv 2022.07.21.500988

Insert

His8OphMA∆C6-TEV18mer

Tags

His8 tagged

Expression

Bacterial

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Avs4_Archaeon_isolate_HyVt-335

Plasmid

#188800

Purpose

Avs4 from Archaeon isolate HyVt-335

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

Avs4

Expression

Bacterial

Promoter

lac

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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KBB382

Plasmid

#185085

Purpose

HA-tagged POM152 with glycosylation sites mutated

Depositor

Ken Belanger

Article

Belanger et al Genetics. 2005 Nov;171(3):935-47. doi: 1

Insert

POM152

Tags

HA

Expression

Yeast

Mutation

Pom152 N280A, N398A, N???A, N???A = Pom152Dglyc

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only
pXCX-sGFAP-DLDH-IRES-tdTomato

Plasmid

#178311

Purpose

Express the bacterial D-lactate dehydrogenase (DLDH) enzyme in astrocytes

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

Insert

D-lactate dehydrogenase

Use

Adenoviral

Tags

tdTomato

Expression

Mammalian

Promoter

sGFAP

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huPxCas12a

Plasmid

#174675

Purpose

Expresses 6xHis-MBP tagged PxCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huPxCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huPrCas12a

Plasmid

#174674

Purpose

Expresses 6xHis-MBP tagged PrCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huPrCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2005

Plasmid

#139880

Purpose

Complementation plasmid for LetAB with 2 ring (rings17) LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(delta161-747)

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2004

Plasmid

#139889

Purpose

Expression plasmid for LetAB with 6 ring (rings 123457) LetB (without TM region) mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; delta634-746)-6xHis

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL1993

Plasmid

#139897

Purpose

Expression plasmid for LetB PLL1 deletion (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; delta114-128, P113G)-6xHis

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL1999

Plasmid

#139903

Purpose

Expression plasmid for LetB PLL7 deletion (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; delta816-826, S815G, T827G)-6xHis

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pWSK29-wzzBST

Plasmid

#172968

Purpose

pWSK29 with Salmonella typhimurium wzzBST gene

Depositor

Renato Morona

Article

Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

Insert

wzzB gene from Salmonella enterica serovar Typhimurium

Expression

Bacterial

Mutation

K198E- please see depositor comments

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pWSK29-fepEST

Plasmid

#172969

Purpose

pWSK29 with Salmonella typhimurium fepEST gene

Depositor

Renato Morona

Article

Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

Insert

fepE gene from Salmonella enterica serovar Typhimurium

Expression

Bacterial

Mutation

G160S- please see depositor comments

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-zfTpo

Plasmid

#168198

Purpose

Express zebrafish thrombopoietin (Tpo), mRNA synthesis

Depositor

Petr Bartunek

Article

Svoboda et al Blood. 2014 Jul 10;124(2):220-8. doi: 10

Insert

Thrombopoietin (thpo Zebrafish)

Use

Vertebrate expression (zebrafish, chicken, xenopu…

Expression

Mammalian

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2298

Plasmid

#164603

Purpose

Expression and Complementation plasmid for MlaD (A17F, A20F, V24F)

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE)-MlaD(Ala17Phe, Ala20Phe, Val24P…

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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DT039A

Plasmid

#159400

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23117 (weak) promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.J23117-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

J23117 (weak) promoter

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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DT038A

Plasmid

#159399

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23106 (medium) promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.J23106-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

J23106 (medium) promoter

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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DT040A

Plasmid

#159401

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli hybrid J23117-J23106 promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.J23117/J23106-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

hybrid J23117-J23106 promoter

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pEM-HypaCas9-D1135E

Plasmid

#154930

Purpose

aTc inducible HypaCas9 with D1135E mutation

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

HypaCas9-D1135E

Use

CRISPR and Synthetic Biology

Tags

ssrA

Expression

Bacterial

Mutation

N692A/M694A/Q695A/H698A/D1135E

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEM-Cas9HF1-D1135E

Plasmid

#154924

Purpose

aTc inducible Cas9HF1 with D1135E mutation

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

Cas9HF1-D1135E

Use

CRISPR and Synthetic Biology

Tags

ssrA

Expression

Bacterial

Mutation

N497A/R661A/Q695A/Q926A/D1135E and stop codon on …

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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