We narrowed to 702 results for: Tk1

1. NES-mCherry-PH-BTKx1

   Plasmid

   #183653

   Purpose

   PtdIns(3,4,5)P3 biosensor

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   BTK (BTK Human, Frog)
   

   Use

   Tags

   X. laevis map2k1.L(32-44)-mCherry

   Expression

   Mammalian

   Mutation

   Amino acids 2-170

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. TdLanYFP-AURKA-Aquamarine

   Plasmid

   #228562

   Purpose

   Encodes the AURKA biosensor where LC3B is flanked by the Aquamarine/TdLanYFP donor/acceptor FRET pair and under the control of the CMV promoter.

   Depositor

   Giulia Bertolin

   Article

   Bousmah et al ACS Sens. 2021 Nov 26;6(11):3940-3947. d

   Insert

   AURKA (AURKA, AURKA Human)
   

   Use

   Tags

   Aquamarine and TdLanYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. AURKA-Aquamarine

   Plasmid

   #228564

   Purpose

   Encodes a donor-only, Aquamarine-tagged version of the AURKA biosensor under the control of a CMV promoter

   Depositor

   Giulia Bertolin

   Article

   Bousmah et al ACS Sens. 2021 Nov 26;6(11):3940-3947. d

   Insert

   AURKA (AURKA, AURKA Human)
   

   Use

   Tags

   Aquamarine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. HCP8

   Plasmid

   #166110

   Purpose

   This plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Whi5

   Depositor

   Matthias Heinemann

   Insert

   Whi5-sg106 (WHI5 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. HCP9

   Plasmid

   #166111

   Purpose

   This plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Hta2

   Depositor

   Matthias Heinemann

   Insert

   Hta2-sg18 (HTA2 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. FUW-hTie1-FLAG

   Plasmid

   #119233

   Purpose

   Plasmid encodes FLAG-tagged full-length human Tie1 gene cloned in lentiviral vector.

   Depositor

   Kari Alitalo

   Insert

   TIE1 (TIE1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   P hUbC

   Available since

   Dec. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRD503

   Plasmid

   #220788

   Purpose

   Overexpresses HTkC in E. coli cells

   Depositor

   Remus Dame

   Article

   Schwab et al bioRxiv 2023.06.01.543357

   Insert

   HTkC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promotor

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. HCP5

   Plasmid

   #166107

   Purpose

   This plasmid encodes a Cas9 protein as well as a sgRNAs that targets the C-terminus of Cyr1.

   Depositor

   Matthias Heinemann

   Insert

   Cyr1CT-sgRNA (CYR1 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. R777-E157 Hs.PDGFRB

   Plasmid

   #70441

   Purpose

   Gateway ORF clone of human PDGFRB [NM_002609.3] with stop codon (for native or N-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   PDGFRB (PDGFRB Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. FUW-TIE1-Cherry

    Plasmid

    #119236

    Purpose

    Plasmid encodes full-length human Tie1 gene fused in-frame with Cherry cloned in lentiviral vector.

    Depositor

    Kari Alitalo

    Insert

    TIE1-mCherry (TIE1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    P hUbC

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. HCP7

    Plasmid

    #166109

    Purpose

    This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Whi5 and the other targets the C-terminus of Hta2.

    Depositor

    Matthias Heinemann

    Insert

    Whi5-sg106 (WHI5 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. R777-E158 Hs.PDGFRB-nostop

    Plasmid

    #70442

    Purpose

    Gateway ORF clone of human PDGFRB [NM_002609.3] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PDGFRB (PDGFRB Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. HCP1

    Plasmid

    #166103

    Purpose

    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination.

    Depositor

    Matthias Heinemann

    Insert

    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HCP2

    Plasmid

    #166104

    Purpose

    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination

    Depositor

    Matthias Heinemann

    Insert

    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HCP3

    Plasmid

    #166105

    Purpose

    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination

    Depositor

    Matthias Heinemann

    Insert

    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FUW-hTie1-ECD-FLAG

    Plasmid

    #119235

    Purpose

    Plasmid encodes FLAG-tagged extracellular domain of human Tie1 gene cloned in lentiviral vector.

    Depositor

    Kari Alitalo

    Insert

    TIE1-ECD-FLAG (TIE1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Contains extracellular domain (ECD) only

    Promoter

    P hUbC

    Available since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. AXL pBABE

    Plasmid

    #105936

    Purpose

    retroviral expression of AXL

    Depositor

    Rosa Marina Melillo

    Insert

    AXL (AXL Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. R777-E297 Hs.RPS6KB1

    Plasmid

    #70581

    Purpose

    Gateway ORF clone of human RPS6KB1 [NM_003161.3] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    RPS6KB1 (RPS6KB1 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. R777-E298 Hs.RPS6KB1-nostop

    Plasmid

    #70582

    Purpose

    Gateway ORF clone of human RPS6KB1 [NM_003161.3] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    RPS6KB1 (RPS6KB1 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZNF175-donor

    Plasmid

    #112347

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZNF175 with GFP

    Depositor

    Kevin White

    Insert

    ZNF175 homology arms flanking EGFP-IRES-Neo cassette (ZNF175 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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