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Plasmid#169408PurposeGateway destination vector for cell-free and mamalian expression with C-term mCherry, 8xHis and Myc tagDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only
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IZ702: pMVP (L3-L2) myc epitope tag + WPRE
Plasmid#121760PurposepMVP L3-L2 entry plasmid, contains myc epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.DepositorInsertmyc epitope tag + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-HaloTag-Puromycin
Plasmid#167208PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with HaloTag and selection with PuromycinDepositorTypeEmpty backboneUseCRISPRTagsHaloTagExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-mClover3-Puromycin
Plasmid#167205PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mClover3 and selection with PuromycinDepositorTypeEmpty backboneUseCRISPRTagsmClover3ExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-NanoLuc-Puromycin
Plasmid#167209PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with NanoLuc and selection with PuromycinDepositorTypeEmpty backboneUseCRISPRTagsNanoLucExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC deltaCTD_P2A_Hygro_Barcode
Plasmid#120511PurposeBarcoded lentiviral vector to express MYC deltaCTD in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC deltaCTD (MYC Human)
UseLentiviralTagsExpressionMutationDeletion of carboxy-terminal domainPromoterEF1aAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC deltaNTD_P2A_Hygro_Barcode
Plasmid#120510PurposeBarcoded lentiviral vector to express MYC deltaNTD in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC deltaNTD (MYC Human)
UseLentiviralTagsExpressionMutationDeletion of amino-terminal domainPromoterEF1aAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC deltaMBI_P2A_Hygro_Barcode
Plasmid#120504PurposeBarcoded lentiviral vector to express MYC deltaMBI in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC deltaMBI (MYC Human)
UseLentiviralTagsExpressionMutationDeletion of MYC Box I domainPromoterEF1aAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC deltab_P2A_Hygro_Barcode
Plasmid#120507PurposeBarcoded lentiviral vector to express MYC deltab in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC deltab (MYC Human)
UseLentiviralTagsExpressionMutationDeletion of basic motifPromoterEF1aAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC deltaLZ_P2A_Hygro_Barcode
Plasmid#120509PurposeBarcoded lentiviral vector to express MYC deltaLZ in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC deltaLZ (MYC Human)
UseLentiviralTagsExpressionMutationDeletion of leucine zipper motifPromoterEF1aAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC deltaHLH_P2A_Hygro_Barcode
Plasmid#120508PurposeBarcoded lentiviral vector to express MYC deltaHLH in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC deltaHLH (MYC Human)
UseLentiviralTagsExpressionMutationDeletion of helix-loop-helix motifPromoterEF1aAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC Thr58Ala_P2A_Hygro_Barcode
Plasmid#120513PurposeBarcoded lentiviral vector to express MYC Thr58Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC Thr58Ala (MYC Human)
UseLentiviralTagsExpressionMutationPoint mutation changing Threonine to Alanine at a…PromoterEF1aAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC Ser62Ala_P2A_Hygro_Barcode
Plasmid#120514PurposeBarcoded lentiviral vector to express MYC Ser62Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC Ser62Ala (MYC Human)
UseLentiviralTagsExpressionMutationPoint mutation changing Serine to Alanine at amin…PromoterEF1aAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC Glu39Ala_P2A_Hygro_Barcode
Plasmid#120512PurposeBarcoded lentiviral vector to express MYC Glu39Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC Glu39Ala (MYC Human)
UseLentiviralTagsExpressionMutationPoint mutation changing Glutamic Acid to Alanine …PromoterEF1aAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPI472
Plasmid#113210Purposeintegrating expression vector, C-terminal mTurquoise2 taggingDepositorTypeEmpty backboneUseDictyostelium discoideum integrating expression v…TagsmTurquoise2ExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPI469
Plasmid#113206PurposeInducible expression vector (dox-on), C-terminal mTurqoise2 taggingDepositorTypeEmpty backboneUseDictyostelium discoideum inducible expression vec…TagsmTurquoise2ExpressionMutationPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOSV809
Plasmid#126602Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOSV805
Plasmid#126598Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOSV802
Plasmid#126595Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOSV807
Plasmid#126600Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only