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We narrowed to 6,954 results for: pcDNA3.1+

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Showing: 261 - 280 of 6954 results

  5. pcDNA-dCas9

    Plasmid
    #47106
    Purpose
    Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells
    Depositor
    Charles Gersbach
    Publication
    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR
    Tags
    Flag and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically inactive)
    Promoter
    CMV
    Available since
    Nov. 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    12 Articles
    47106-plasmid-map-sequence-id-433987

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  6. pcDNA3-GFP-LC3-RFP-LC3ΔG

    Plasmid
    #168997
    Purpose
    Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells and zebrafish to measure autophagic flux
    Depositor
    Noboru Mizushima
    Publication
    Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    Tags
    EGFP and mRFP1
    Expression
    Mammalian
    Available since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    9 Articles
    168997-plasmid-map-sequence-id-414009

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  12. PCDNA3-FlipGFP(Casp3 cleavage seq) T2A mCherry

    Plasmid
    #124428
    Purpose
    Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in mammalian cells
    Depositor
    Xiaokun Shu
    Publication
    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FlipGFP(Casp3 cleavage seq) T2A mCherry
    Expression
    Mammalian
    Available since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    124428-plasmid-map-sequence-id-322471

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  13. pcDNA3-SARS-CoV-2-S-RBD-sfGFP

    Plasmid
    #141184
    Purpose
    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with sfGFP
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] (S SARS coronavirus 2)
    Tags
    Signal peptide from influenza HA and Superfolder …
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    21 Articles
    141184-plasmid-map-sequence-id-306284

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  16. TRUPATH

    Plasmid kit
    #1000000163
    Purpose
    BRET biosensor plasmids encoding alpha, beta, or gamma subunits of the heterotrimeric G protein complex for detection of heterotrimer dissociation.
    Depositor
    Bryan Roth
    Application
    Gene Expression and Labeling
    Vector type
    Mammalian Expression
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only

  17. C1(1-29)-miniTurbo-V5_pCDNA3

    Plasmid
    #107174
    Purpose
    expresses V5-tagged miniTurbo on the mammalian ER membrane
    Depositor
    Alice Ting
    Publication
    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    miniTurbo (BirA mutant)
    Expression
    Mammalian
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Available since
    May 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    107174-plasmid-map-sequence-id-205307

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  18. pcDNA3-TLR1-YFP

    Plasmid
    #13014
    Depositor
    Doug Golenbock
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Toll-Like Receptor 1 (TLR1 Human)
    Tags
    YFP
    Expression
    Mammalian
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    13014-plasmid-map-sequence-id-237573

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  19. pcDNA-dCas9-p300 Core (D1399Y)

    Plasmid
    #61358
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    14 Articles
    61358-plasmid-map-sequence-id-99789

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Showing: 261 - 280 of 6954 results