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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 952 results for: trna

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Showing: 261 - 280 of 952 results

  15. pXPR-SF61

    Plasmid
    #163916
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of para-Pentafluorosulfanyl-Phenylalanine, into proteins in E. coli in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Article
    Qianzhu et al J Am Chem Soc. 2020 Oct 14;142(41):17277
    Insert
    SF5Phe tRNA synthetase
    Expression
    Bacterial
    Promoter
    araBAD
    Available Since
    March 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    163916-plasmid-map-sequence-id-327914

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  16. pEDF-PhdRS

    Plasmid
    #127445
    Purpose
    Double mutant of wild type mmPylRS designed for incorporation of non-cannonical amino acids in to M13 bacteriophage
    Depositor
    Wenshe Liu
    Article
    Tharp et al Nat Commun. 2020 Mar 13;11(1):1392. doi:
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Expression
    Bacterial
    Mutation
    N346A-C348A mutations on PylRS
    Promoter
    araBAD
    Available Since
    April 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    127445-plasmid-map-sequence-id-250213

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  18. pIND4-RC-M210TAG-A7

    Plasmid
    #223015
    Purpose
    Expresses 3 subunits of photosynthetic reaction center from R. sphaeroides and aaRS-tRNA pair for 3-nitrotyrosine
    Depositor
    Steven Boxer
    Inserts
    puhA
    pufL
    pufM
    A7 RS
    pylT tRNA
    Tags
    6xHis
    Mutation
    changed Tyr M210 to amber stop codon (TAG) for am…
    Promoter
    rrnB
    Available Since
    Sept. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    223015-plasmid-map-sequence-id-444740

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  19. pIND4-RC-C2-MmXYRS

    Plasmid
    #222744
    Purpose
    Expresses 3 subunits of photosynthetic reaction center from R. sphaeroides and aaRS-tRNA pair for incorporating haloalkane crosslinker
    Depositor
    Steven Boxer
    Inserts
    puhA
    pufL
    pufM
    MmXYRS
    Mm Pyl tRNA
    Tags
    6xHis
    Mutation
    Contains the following substitutions: V346A, W348…
    Promoter
    rrnB
    Available Since
    Sept. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    222744-plasmid-map-sequence-id-444741

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  20. pEVOL-NBK-1

    Plasmid
    #207639
    Purpose
    A plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli.
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    Pyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)
    Expression
    Bacterial
    Mutation
    The evolved synthetase NBK-1 contains mutations Y…
    Promoter
    araBAD
    Available Since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207639-plasmid-map-sequence-id-409890

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Showing: 261 - 280 of 952 results