-
Plasmid#195213PurposeMammalian expression vector for GFP tagged NHERF1.DepositorInsertNHERF1 (NHERF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
gRNA_GFP-T2
Plasmid#41820PurposeExpresses a guide RNA (gRNA) to target GFP (T2 target sequence) for genome engineeringDepositorInsertgRNA_GFP-T2
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOO2-GW
Plasmid#135097PurposeGateway vector for expression of proteins in Xenopus oocytesDepositorTypeEmpty backboneUseBacterial expression vector for generating sp6 tr…TagsExpressionMutationPromoterSP6Available sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
BFP-Rab5CA (Q79L)
Plasmid#205705PurposeMammalian expression of BFP-tagged mutant Rab5CADepositorInsertRAB5A (RAB5A Human)
UseTagsExpressionMammalianMutationQ79LPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-Fv2E-flt4um200-ns
Plasmid#67696Purposemiddle entry plasmid with dimerizable extracellular domain fused to um200 mutant Danio Flt4 cytoplasmic domainDepositorInsertFv2E-flt4um200
UseTagsHAExpressionMutationum200, no stop codon; N1056SPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
ciCas9_pcDNA5
Plasmid#100550PurposeExpresses ciCas9 in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.DepositorInsertciCas9
UseCRISPR; Frt, flp-inTagsFLAGExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-pOTC-3H-GFP
Plasmid#205725PurposeExpression of GFP fused with pOTC-3H, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase and a thermostable three helix-bundleDepositorInsertpOTC-3H-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
hPRL3_1-169
Plasmid#209796PurposeBacterial expression of human PRL-3 without farnesylation motifDepositorInsertPRL-3 (PTP4A3 Human)
UseTags6xHisExpressionBacterialMutationaa 1-169 onlyPromoterT7Available sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
786 pCSmAKT2Acherry
Plasmid#31167DepositorInsertAkt
UseExpression vectorTagsMyr and mCherryExpressionMutationmembrane tethered AktPromoterAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
TtGNN-GFP-empty
Plasmid#106380Purposedox inducible expression of GFP with neo resistanceDepositorInsertGFP
UseRetroviralTagsExpressionMammalianMutationPromoterTRE-tightAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-6MTzactMap2k2b
Plasmid#67710PurposeMiddle entry cloneDepositorInsertactivated Map2k2b (map2k2b Zebrafish)
UseTagsExpressionMutationS221D, S225DPromoternoneAvailable sinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKJ458
Plasmid#228709PurposeMammalian expression of Antibody C143 HC R39K (heavy chain); to be used with pKJ460 (Plasmid 228710) to make the antibodyDepositorArticleInsertC143 HC R39K
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKJ460
Plasmid#228710PurposeMammalian expression of Antibody C143 LC N28R (light chain); to be used with pKJ458 (Plasmid 228709) to make the antibodyDepositorArticleInsertC143 LC N28R
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKJ423
Plasmid#228708PurposeMammalian expression of Anti-CD71 LC (light chain); to be used with pKJ455 (Plasmid 228707) to make the antibodyDepositorArticleInsertAnti-CD71 LC
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXOOY
Plasmid#196449PurposeA dual vector for expression of membrane proteins in S. cerevisiae and X. laevisDepositorTypeEmpty backboneUseExpression in xenopus laevisTagsExpressionYeastMutationPromoteryeast CYC-GAL and T7 promoterAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-pOTC-GFP
Plasmid#205724PurposeExpression of GFP fused with pOTC, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylaseDepositorInsertpOTC-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPEPT1
Plasmid#195001PurposeProduction of peptides in E. coli as GB1 fusionDepositorTypeEmpty backboneUseTagsHis8-tag and StrepTagII-GB1ExpressionBacterialMutationPromoterAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GVcl 1-1066 /*TBM
Plasmid#162781Purposeamplification of Gallus gallus Vcl mutated in Tankyrase Binding Domain IIDepositorArticleInsertGallus gallus vinculin (VCL Chicken)
UseTagsExpressionBacterialMutationchanged Gly 454 to ValPromoterAvailable sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
EMX1*_sgRNA
Plasmid#100558PurposeExpresses EMX1* sgRNA. Target sequence: (G)GAGTCCGAGCAGAAGAAGAA. Same target sequence as #100555, except with an additional 5' G.DepositorInsertEMX1 sgRNA
UseTagsExpressionMutationPromoterU6Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pME-Fv2E-flt4-ns
Plasmid#67695Purposemiddle entry plasmid with dimerizable extracellular domain fused to Danio Flt4 cytoplasmic domainDepositorInsertFv2E-flt4
UseTagsExpressionMutationno stop codon; D1302E (see depositor's comme…PromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only