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Wnt7A Non-STOP

Plasmid

#35896

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt7A (WNT7A Human)

Use

Entry vector

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoMaxGFP-Kan

Plasmid

#44907

Purpose

Yeast tagging vector to create gene fusion with yeast optimized MaxGFP with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoMaxGFP

Expression

Yeast

Available Since

May 17, 2013

Availability

Academic Institutions and Nonprofits only

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Wnt6 STOP

Plasmid

#35876

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt6 (WNT6 Human)

Use

Entry vector

Mutation

Silent Mutation G (567) --> A

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Wnt8B STOP

Plasmid

#35880

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt8B (WNT8B Human)

Use

Entry vector

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Wnt16 STOP

Plasmid

#35886

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt16 (WNT16 Human)

Use

Entry vector

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pME-basGal80

Plasmid

#51621

Purpose

vertebrate expression of Gal80 preceded by a basal promoter

Depositor

Joshua Bonkowsky

Article

Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

Insert

Gal80 (GAL80 Budding Yeast)

Use

Vertebrate expression

Promoter

basal promoter

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only
M3939 leu2::ADE2 Disruptor Converter

Plasmid

#51672

Purpose

Yeast marker swap plasmid for converting LEU2 to ADE2

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

ADE2 (ADE2 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Promoter

ADE2

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pDR-meptrac-H194E

Plasmid

#47764

Purpose

expresses MepTrac-H194E in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

MepTrac-H194E

Tags

mcpGFP

Expression

Yeast

Mutation

H194E

Promoter

PMA

Available Since

Sept. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pFP125

Plasmid

#21674

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

LacZ (lacZ E. coli)

Use

Centromeric plasmid

Mutation

The second lacZ sequence has been completely dele…

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pJC45BS104 L892W

Plasmid

#1308

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 L892W (HSP104 Budding Yeast)

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Leu 892 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pCS2 xenopus twisted gastrulation

Plasmid

#24974

Depositor

Edward De Robertis

Article

Larrain et al Development. 2001 Nov . 128(22):4439-47.

Insert

twisted gastrulation (Twsg1 Mouse)

Tags

FLAG and chordin signal peptide

Expression

Mammalian

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
TAL3577

Plasmid

#47296

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-tetmethylcytosinedioxygenase3(previous si:ch211-220a11.1)-right

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only
TAL3576

Plasmid

#47295

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-tetmethylcytosinedioxygenase3(previous si:ch211-220a11.1)-left

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 cand181

Plasmid

#26323

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

cand181

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pRS414-54

Plasmid

#23084

Depositor

Sharon Dent

Article

Tripic et al Biochem Biophys Res Commun. 2006 Jan 20.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysines 4 and 9 to Glutamines,…

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pJD100

Plasmid

#23085

Depositor

Sharon Dent

Article

Tripic et al Biochem Biophys Res Commun. 2006 Jan 20.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysine 4 to Glutamine, H3 K4Q.

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pJD101

Plasmid

#23086

Depositor

Sharon Dent

Article

Tripic et al Biochem Biophys Res Commun. 2006 Jan 20.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysine 4 to Arginine, H3 K4R.

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
p316CUPSup35GFPSG

Plasmid

#1224

Depositor

Susan Lindquist

Article

Liu et al Proc Natl Acad Sci U S A 2002 Dec 10;99

Insert

SUP35 (SUP35 Budding Yeast)

Tags

GFPSG S65T V164A

Expression

Yeast

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pTOPO-rRNA-5‘ETS 347 to 631

Plasmid

#23994

Depositor

Susan Baserga

Article

Gallagher et al Genes Dev. 2004 Oct 15. 18(20):2506-17.

Insert

rRNA 5'ETS ntds 347 to 631

Use

Bacterial cloning - topo

Mutation

None

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only
pGC97

Plasmid

#19629

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)

Use

Flp/frt, destination vector

Expression

Worm

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pGC238

Plasmid

#19631

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)

Use

Flp/frt, destination vector

Tags

4xNLS

Expression

Worm

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pXN-FBLWLF-GAL4AD

Plasmid

#26263

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

Gal4AD-Zip+ (GAL4 Budding Yeast)

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pJC45BS104 F130W

Plasmid

#1302

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 F130W (HSP104 Budding Yeast)

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Phe 130 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 SSA1

Plasmid

#19516

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

SSA1 (SSA1 Budding Yeast)

Tags

V5

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pJRL2 PHO5(UASm1)pr VYFP (EB#1549)

Plasmid

#14841

Depositor

Erin O'Shea

Article

Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

Insert

PHO5 promoter UASm1 (PHO5 Budding Yeast)

Tags

VYFP

Expression

Yeast

Mutation

UASm1 (AAGCTT was substituted for CACGTT). Unindu…

Available Since

May 25, 2007

Availability

Academic Institutions and Nonprofits only
pET21a.TM1363

Plasmid

#11420

Depositor

Sung-Hou Kim

Article

Shin et al J Mol Biol. 2004 Jul 30. 341(1):227-39.

Insert

peptide release factor I (prfA Thermotoga maritima)

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pSB819-PKR-ora

Plasmid

#20027

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R

Expression

Yeast

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pJC45BS104 L553W

Plasmid

#1306

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 L553W (HSP104 Budding Yeast)

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Leu 553 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pLCHKO-CRASP

Plasmid

#231928

Purpose

Empty backbone for CRASP-Seq experiments

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Behera et al bioRxiv [Preprint]. 2025 Apr 26:2025.04.

Type

Empty backbone

Use

Lentiviral

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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EKAREN5-CFPonly

Plasmid

#246759

Purpose

Control plasmid for EKAREN5 without YPet cassette (expressing CFP only)

Depositor

Toru Hiratsuka

Article

Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.

Insert

EKAREN5-CFPonly

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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EKAREN5-YFPonly

Plasmid

#246760

Purpose

Control plasmid for EKAREN5 without mTurquioise2 cassette (expressing YFP only)

Depositor

Toru Hiratsuka

Article

Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.

Insert

EKAREN5-YFPonly

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pHR Myc-Oskar-FusionRed-FRB-iLID

Plasmid

#223289

Purpose

The Oskar germ granule protein tagged with optogenetic and chemogenetic tags for recruitment of desired cargo. Used to demonstrate condensate dissolution using the OptoMBP system.

Depositor

Jared Toettcher

Article

Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

Insert

myc-Oskar-FusionRed-FRB-iLID

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pET-hisM5RT

Plasmid

#216672

Purpose

Expresses his-tagged Mut5 reverse transcriptase in E. coli hosts

Depositor

Po-Min Chiang

Article

Hsueh et al PLoS Biol. 2025 Dec 15;23(12):e3003537.

Insert

thermostable M-MuLV reverse transcriptase variants

Tags

his-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pKG0618_pPV1-CMV

Plasmid

#239688

Purpose

BsaI Golden Gate asembly plasmid encoding the CMV promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

CMV promoter

Use

Synthetic Biology

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG1179_pPV1-EFS

Plasmid

#239690

Purpose

BsaI Golden Gate assembly plasmid encoding the EFS promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

EFS promoter

Use

Synthetic Biology

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pJL178

Plasmid

#243687

Purpose

Stably expresses fusion of SUPV3L1(1-114aa) and ALDH18A1(106-795aa)

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

UTRs and CDS of SUPV3L1-(1-114aa)-ALDH18A1(106-795aa)

Use

Lentiviral

Promoter

EF1a

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pCK303.306

Plasmid

#242926

Purpose

Expression of an MS2 scRNA J306

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

scRNA MS2 J306

Use

CRISPR and Synthetic Biology

Promoter

Bba_J23119

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCK677.102-110

Plasmid

#242928

Purpose

Expression of a boxB scRNA J102 and an MS2 scRNA J110

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

scRNA MS2 J110

Use

CRISPR and Synthetic Biology

Promoter

Bba_J23119

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCK662.-1

Plasmid

#242922

Purpose

Reporter J1-pspAp-mRFP with 1bp deletion phase-shift

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

J1-pspAp.-1

Use

CRISPR and Synthetic Biology

Promoter

pspAp.-1

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH105

Plasmid

#243726

Purpose

Expresses PMPCA

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

PMPCA (PMPCA Human)

Use

Lentiviral

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF myc-TurboID

Plasmid

#245241

Purpose

Proximity-labeling proteomics negative control

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Mar 21:2025.02.

Insert

TurboID

Expression

Mammalian

Mutation

wild-type

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pKO023

Plasmid

#242923

Purpose

Reporter J1-J3(122)-BBa_J23117 with Sth1 PAM

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

J1-J3(122)

Use

CRISPR and Synthetic Biology

Promoter

Bba_J23117

Available Since

Sept. 29, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAK011

Plasmid

#242920

Purpose

Reporter J1-J3(122) -BBa_J23117-mRFP

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

J1-J3(122)

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23117

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHRSV_CHARMS-2xTIR

Plasmid

#233930

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-2xTIR which contain a tandem dimer of TIR domains

Depositor

Marcus Taylor

Article

Lichtenstein et al bioRxiv 2024.05.08.593169

Insert

CHARMS-2xTIR

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

Aug. 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
16_GP_Vps4p_L7C_A43C

Plasmid

#203356

Purpose

Vps4 with stabilized MIT domain and 16aa GlyPro N-terminal extension

Depositor

Chris Hill

Article

Wienkers et al Sci Rep. 2025 Jul 11;15(1):25024. doi: 1

Insert

Vps4p

Tags

6x-HIS-TEV and Gly Pro linker (16aa)

Expression

Bacterial

Mutation

L7C and A43C

Promoter

LacO

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
2xPeptide 8_Vps4p_L7C_A43C

Plasmid

#203358

Purpose

Vps4 with stabilized MIT domain with an N-terminal extension of 16 binding amino acids

Depositor

Chris Hill

Article

Wienkers et al Sci Rep. 2025 Jul 11;15(1):25024. doi: 1

Insert

Vps4p

Tags

2xPeptide 8 and 6x-HIS-TEV

Expression

Bacterial

Mutation

L7C and A43C

Promoter

LacO

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Vps4p_GP_linker

Plasmid

#203361

Purpose

Vps4 with GlyPro linker between MIT domain and Large ATPase Domain

Depositor

Chris Hill

Article

Wienkers et al Sci Rep. 2025 Jul 11;15(1):25024. doi: 1

Insert

Vps4

Tags

6x-HIS-TEV

Expression

Bacterial

Mutation

Residues 83-111 are replaced with Gly Pro linker

Promoter

LacO

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2499_pGEEC531_pShip-CMV-mRuby2-WPRE

Plasmid

#239727

Purpose

Plasmid expressing mRuby2 with the CMV promoter and WPRE 3' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2906_pGEEC546_pShip-EFS-tagBFP-bGH

Plasmid

#239734

Purpose

Plasmid expressing tagBFP with the EFS promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

tagBFP

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2907_pGEEC547_pShip-hPGK-tagBFP-bGH

Plasmid

#239735

Purpose

Plasmid expressing tagBFP with the hPGK promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

tagBFP

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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