We narrowed to 8,094 results for: Dos;

1. pDM068

   Plasmid

   #216811

   Purpose

   Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and NAT selectable marker.

   Depositor

   Ken Sawin

   Article

   Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

   Type

   Empty backbone

   Use

   CRISPR; Fungal expression

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. RT-4

   Plasmid

   #220443

   Purpose

   Construct used to generate the PUF3/PUP2/3HA tagging strains. Surrounding the PUP2/3HA/URA3 are large regions of homology to integrate at the 3’ end of the PUF3 gene.

   Depositor

   Marvin Wickens

   Article

   Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

   Insert

   PUF3 (PUF3 Budding Yeast)
   

   Use

   Tags

   3xHA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV hSyn mRuby

   Plasmid

   #220912

   Purpose

   Neuronal specific expression of mRuby

   Depositor

   Timothy Ryan

   Article

   Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

   Insert

   mRuby
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCPH3.1-GS3

   Plasmid

   #204762

   Purpose

   Expression of Cas9 and human H3.1

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H3.1 (H3C6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCPH2A-GS3

   Plasmid

   #204753

   Purpose

   Expression of Cas9 and human H2A

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2A (H2AC20 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCPH2A.J-GS3

   Plasmid

   #204757

   Purpose

   Expression of Cas9 and human H2A.J

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2AJ (H2AJ Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. TDP-43 mCerulean

   Plasmid

   #216227

   Purpose

   TDP-43 with a C-terminal mCerulean tag for expression in mammalian cells.

   Depositor

   Jennifer Lee

   Article

   Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1_Zeo_3xSH2(GRB2)-CTEV-2xHA

   Plasmid

   #214614

   Purpose

   adapter for RTK split TEV recruitment assays

   Depositor

   Michael Wehr

   Article

   Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

   Insert

   3xSH2 domains of GRB2-CTEV (GRB2 Human)
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3_ERBB4-NTEV-tcs-GV-2xHA

   Plasmid

   #214613

   Purpose

   ERBB4 receptor for split TEV assay

   Depositor

   Michael Wehr

   Article

   Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

   Insert

   ERBB4-NTEV (ERBB4 Human)
   

   Use

   Tags

   TEV-Gal4-VP16-2xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3_EGFR-NTEV-tcs-GV-2xHA

    Plasmid

    #214610

    Purpose

    EGFR receptor for split TEV assay

    Depositor

    Michael Wehr

    Article

    Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

    Insert

    EGFR-NTEV (EGFR Human)
    

    Use

    Tags

    TEV-Gal4-VP16-2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPI230

    Plasmid

    #207431

    Purpose

    Safe haven knock-in plasmid for the act5 locus for C-terminal mNeonGreen fusions

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Insert

    C-terminal mNeonGreen
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPI231

    Plasmid

    #207432

    Purpose

    Safe haven knock-in plasmid for the act5 locus for N-terminal mNeonGreen fusions

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Insert

    N-terminal mNeonGreen
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL2084

    Plasmid

    #137726

    Purpose

    Gateway vector for inducible stem loop RNAi in T.b. brucei 2T1

    Depositor

    Jeremy Mottram

    Article

    Jones et al PLoS Pathog. 2014 Jan;10(1):e1003886. do

    Type

    Empty backbone

    Use

    RNAi; Trypanosoma brucei tet inducible stem loop

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenti-Cas9-sgHPRT1

    Plasmid

    #196713

    Purpose

    CRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TG

    Depositor

    Bernhard Schmierer

    Article

    Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

    Insert

    Cas9-T2A-BSD-U6-sgHPRT1
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a/hU6

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CMV-CcuCas9

    Plasmid

    #192127

    Purpose

    Expresses CcuCas9

    Depositor

    Yongming Wang

    Article

    Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

    Insert

    CcuCas9
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-MS2-HA-HsNanos3_X

    Plasmid

    #147944

    Purpose

    Mammalian Expression of HsNanos3

    Depositor

    Elisa Izaurralde

    Article

    Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

    Insert

    HsNanos3 (NANOS3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-MS2-HA-HsNanos3-F4A_X

    Plasmid

    #147946

    Purpose

    Mammalian Expression of HsNanos3-F4A

    Depositor

    Elisa Izaurralde

    Article

    Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

    Insert

    HsNanos3-F4A (NANOS3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-MS2-HA-HsNanos3-W7E_X

    Plasmid

    #147947

    Purpose

    Mammalian Expression of HsNanos3-W7E

    Depositor

    Elisa Izaurralde

    Article

    Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

    Insert

    HsNanos3-W7E (NANOS3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-MS2-HA-HsNanos3-Y10E_X

    Plasmid

    #147948

    Purpose

    Mammalian Expression of HsNanos3-Y10E

    Depositor

    Elisa Izaurralde

    Article

    Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

    Insert

    HsNanos3-Y10E (NANOS3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pN1-HsSmg5_267-1017-V5-SBP-MBP_V

    Plasmid

    #147762

    Purpose

    Mammalian Expression of HsSmg5_267-1017

    Depositor

    Elisa Izaurralde

    Article

    Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

    Insert

    HsSmg5_267-1017 (SMG5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation C969T compared to the sequenc…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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