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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 9,532 results for: SUB

Showing: 7001 - 7050 of 9532 results

  10. pCT5-bac1.8

    Plasmid
    #119871
    Purpose
    Constitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coli
    Depositor
    Claudia Schmidt-dannert
    Article
    Seo et al Appl Microbiol Biotechnol. 2018 Nov 3. p
    Inserts
    CymR'
    sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    SNP in 592 bp
    Promoter
    PserA promoter and Pveg promoter with CuO sequence
    Available since
    Jan. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    119871-plasmid-map-sequence-id-232788

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  11. pBRα-β flap (831-1057)

    Plasmid
    #53734
    Purpose
    Expression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.
    Depositor
    Ann Hochschild
    Article
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    alpha NTD-Beta flap fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    53734-plasmid-map-sequence-id-83920

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  12. pJK2020

    Plasmid
    #171932
    Purpose
    express MS2 coat protein (MCP) fused to superfolder GFP and histone subunit H2B fused to mCherry in an operon
    Depositor
    Judith Kimble
    Article
    Lee et al Dev Cell. 2019 Aug 19;50(4):426-435.e4.
    Insert
    Pmex-5::MS2 Coat Protein::linker::sfGFP::tbb-2 3'UTR::gpd-2 intergenic sequence::H2B::mCherry::unc-54 3'UTR
    Use
    Tags
    superfolder GFP, mCherry
    Expression
    Worm
    Mutation
    Promoter
    -
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    171932-plasmid-map-sequence-id-337179

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  24. 1.3xWT HBV-Luciferase

    Plasmid
    #61549
    Purpose
    The 1.3xHBV-Luciferase plasmid to monitor HBV transcription. 1. Luciferase inserted within Core ORF. 2. Luciferase is regulated by EnhII - Core promoter. 3. The functional luciferase ATG is of Core
    Depositor
    Yosef Shaul
    Article
    Doitsh et al Mol Cell Biol. 2004 Feb;24(4):1799-808.
    Insert
    1.3 times of the HBV genome with luciferase within the Core ORF
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Substitution of HBV sequences with luciferase ORF…
    Promoter
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    61549-plasmid-map-sequence-id-212455

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  25. mito-mGarnet2

    Plasmid
    #104310
    Purpose
    codes for a fusion of fluorescent protein mGarnet2 and mito, stains mictochondrial content in live cells, i.e. human cytochrome c oxidase subunit VIII, the mitochondrial targeting sequence mito
    Depositor
    Ulrich Nienhaus
    Article
    Matela et al Chem Commun (Camb). 2017 Jan 10;53(5):97
    Insert
    mito-mGarnet2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    104310-plasmid-map-sequence-id-225636

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  28. mito-mGarnet

    Plasmid
    #104309
    Purpose
    codes for a fusion of fluorescent protein mGarnet and mito, stains mictochondrial content in live cells, i.e. human cytochrome c oxidase subunit VIII, the mitochondrial targeting sequence mito
    Depositor
    Ulrich Nienhaus
    Article
    Hense et al Sci Rep. 2015 Dec 9;5:18006. doi: 10.103
    Insert
    mito-mGarnet
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    104309-plasmid-map-sequence-id-225634

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  45. IBMc460

    Plasmid
    #161940
    Purpose
    Carries Golden Gate substitution insert for IBM with gp41-1 intein. PPhlF as C-lobe promoter
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PPhlF
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    161940-plasmid-map-sequence-id-329041

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  48. pCDM4-GLY

    Plasmid
    #49808
    Purpose
    Encodes pgk, gapA, aceE, aceF, lpdA in pseudo-operon configuration.
    Depositor
    Mattheos Koffas
    Article
    Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc
    Inserts
    phosphoglycerate kinase
    glyceraldehyde-3-phosphate dehydrogenase
    pyruvate dehydrogenase subunit E1
    dihydrolipoamide acetyltransferase
    dihydrolipoamide dehydrogenase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    49808-plasmid-map-sequence-id-74623

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Showing: 7001 - 7050 of 9532 results