We narrowed to 7,241 results for: 11

1. pcDNA-NES-ZapCmR2

   Plasmid

   #59015

   Purpose

   Cytosolic zinc-binding FRET biosensor

   Depositor

   Amy Palmer

   Article

   Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

   Insert

   NES-ZapCmR2 genetically encoded cytosolic Zn(II) sensor
   

   Use

   Tags

   Nuclear Exclusion Signal

   Expression

   Mammalian

   Mutation

   See comment

   Promoter

   CMV

   Available since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pCDNA3 Flag Jnk1b2

   Plasmid

   #13752

   Depositor

   Roger Davis

   Article

   Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

   Insert

   Jnk1b2 (MAPK8 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pOET3-N-TEV-Twin-Strep

   Plasmid

   #203323

   Purpose

   Generate the baculovirus to express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cells

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   TEV cleavable site (ENLYFQG) and Twin-strep-tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGSTag Flag Jnk3a2

   Plasmid

   #15748

   Depositor

   Roger Davis

   Article

   Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

   Insert

   Jnk3a2 (MAPK10 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 6, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pconst #4-RFP

   Plasmid

   #81078

   Purpose

   constitutive promoter expressing RFP, biobrick construction

   Depositor

   Jay Keasling, Paul Adams

   Article

   Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

   Insert

   RFP
   

   Use

   P15a origin, constitutive promoter, kanr

   Tags

   Expression

   Mutation

   Promoter

   Constitutive Promoter - BBa_J23107

   Available since

   Aug. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET22b-T5-FB-E25TAG-F6C

   Plasmid

   #176546

   Purpose

   Expresses IgG affinity protein FB(F6C) with a TAG stop codon at E25 position

   Depositor

   Han Xiao

   Article

   Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

   Insert

   IgG affinity protein
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   change phenylalanine 6 to cysteine; change glutam…

   Promoter

   T5

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDF0163 GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon

   Plasmid

   #172504

   Purpose

   Encodes the GwCas7x3 protein only for bacterial recombinant protein expression for purification

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FL-fibulin-3 pcDNA4

   Plasmid

   #29700

   Depositor

   Mariano Viapiano

   Article

   Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

   Insert

   EFEMP1 (EFEMP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pGL3-NRASlong

   Plasmid

   #13797

   Depositor

   Frank Slack

   Article

   Johnson et al Cell. 2005 Mar 11. 120(5):635-47.

   Insert

   NRAS (NRAS Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pENN.AAV.CB7.CI.PM20D1-flag.WPRE.rBG

    Plasmid

    #132682

    Purpose

    AAV plasmid encoding mouse PM20D1 with C-terminal flag tag

    Depositor

    Jonathan Long

    Article

    Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

    Insert

    Peptidase M20 domain containing 1 (Pm20d1 Mouse)
    

    Use

    AAV

    Tags

    6xHIS and Flag

    Expression

    Mutation

    Promoter

    Chicken b-actin

    Available since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGP-CMV-NES-fRGECO1

    Plasmid

    #125247

    Purpose

    Expresses red fluorescent calcium sensor in mammalian cells

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRGECO2
    

    Use

    Tags

    NES signal

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MTK3a_007

    Plasmid

    #123760

    Purpose

    Encodes Peroxisome Targeting Sequence as a Type 3a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PTS1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDNA3 Flag Jnk2b2

    Plasmid

    #13757

    Depositor

    Roger Davis

    Article

    Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

    Insert

    Jnk2b2 (MAPK9 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

14. NELFE-mOrange pHis-//

    Plasmid

    #53303

    Purpose

    Expresses His-tagged NELF-E-mOrange fusion protein.

    Depositor

    John Lis

    Article

    Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

    Insert

    NELF-E protein (Nelf-E Fly)
    

    Use

    Tags

    His and mOrange

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GST-BRD (1079-1198)

    Plasmid

    #99507

    Purpose

    Bacterial expression of mouse CBP bromodomain

    Depositor

    Peter Wright

    Article

    Park et al FEBS Lett. 2013 Aug 19;587(16):2506-11.

    Insert

    mouse CBP BRD (1079-1198) (Crebbp Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEBG-Pak1 83-149 L107F

    Plasmid

    #12215

    Depositor

    Jonathan Chernoff

    Article

    Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

    Insert

    Pak1 (PAK1 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Autoinhibitory domain (aa 83-149) with L107F muta…

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pEasyG1_zeo

    Plasmid

    #184910

    Purpose

    Template to generate via PCR a single gRNA for expression in S. cerevisiae

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pconst #1-RFP

    Plasmid

    #81075

    Purpose

    constitutive promoter expressing RFP, biobrick construction

    Depositor

    Jay Keasling, Paul Adams

    Article

    Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

    Insert

    RFP
    

    Use

    P15a origin, constitutive promoter, kanr

    Tags

    Expression

    Mutation

    Promoter

    Constitutive Promoter - BBa_J23119

    Available since

    Aug. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-JNKAR1-NES

    Plasmid

    #84632

    Purpose

    Unimolecular, FRET-based JNK activity reporter; cytosol targeted

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    ECFP-FHA1-JNKsubDD-Citrine-NES
    

    Use

    Tags

    6xHis, Citrine, ECFP, Nuclear export signal (NES)…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TN-XXL pRSETB

    Plasmid

    #45796

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Mank et al Nat Methods. 2008 Sep;5(9):805-11. doi:

    Insert

    TN-XXL
    

    Use

    Tags

    6xHIS, ECFP, and YFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 21, 2014

    Availability

    Academic Institutions and Nonprofits only