We narrowed to 14,474 results for: shi

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pEGFP-C1-IFT43

Plasmid

#218735

Purpose

Expresses N-terminally EGFP-tagged IFT43 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

Insert

IFT43 (IFT43 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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INPP5D-eGFP D673G mutant

Plasmid

#161999

Purpose

Mammalian expression vector, containing a GFP tagged phosphatase dead version of INPP5D (D673G)

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

INPP5D (INPP5D Human)

Tags

eGFP

Expression

Mammalian

Mutation

D673G

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pIRESneo-FLAG/HA-Ago2-Y529E

Plasmid

#215842

Purpose

Expression of AGO2-Y529E mutant

Depositor

Kumiko Ui-Tei

Article

Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

Insert

Human AGO2 (AGO2 Human)

Expression

Mammalian

Mutation

Ago2-Y529E

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP9B-HA

Plasmid

#209214

Purpose

Mammalian expression of ATP9B

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

ATP9B (ATP9B Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-neo-myc-LC3(G120A)-HA

Plasmid

#45245

Depositor

Tamotsu Yoshimori

Article

Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

HA and myc

Expression

Mammalian

Mutation

Glycine at position 120 mutated to Alanine

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pMXs-hs-3xHA-delta6xNHL-LIN-41

Plasmid

#52722

Purpose

retroviral expression of human LIN-41 / TRIM71 containing deletion of the 6xNHL repeats and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

deleted amino acids 593–868

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pRD421

Plasmid

#163112

Purpose

Production of His6_TEV-cleavable-linker_mEos3.2_H-NSdbd in BL21 (DE3) pLysS

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

His-tag_TEV-cleavable-linker_mEos3.2_H-NSdbd

Tags

His-tag with TEV-cleavable linker

Expression

Bacterial

Promoter

T7 promoter

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRD395

Plasmid

#163102

Purpose

Expression of mCherry_H-NSdbd in Escherichia coli (and, potentially, other bacteria)

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

mCherry_H-NSdbd

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-3xHA-deltaRING-LIN-41

Plasmid

#52718

Purpose

retroviral expression of human LIN-41 / TRIM71 containing a RING domain deletion and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

deleted amino acids 12-91

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pCI-neo-myc-LC3(deltaC22)

Plasmid

#45448

Depositor

Tamotsu Yoshimori

Article

Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

myc

Expression

Mammalian

Mutation

Encodes amino acids 1-120

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pmyc-GFP-TNRC6A-(935-1026)-NES-mut

Plasmid

#42021

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

contain aa935-1026, F951A, F955A, I958A, F960A

Promoter

CMV

Available Since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-del.GW-I+II+III-NES-mut

Plasmid

#42040

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

deleted aa457-532, aa716-772 and aa804-924, F951A…

Promoter

CMV

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pRD398

Plasmid

#163105

Purpose

Expression of mCherry_H-NSdbd in eukaryotes for visualisation of nuclear DNA

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

NLS_mCherry_H-NSdbd_NLS

Use

Eukaryotic expression

Expression

Mammalian

Promoter

CMV promoter; T7 promoter

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pME-foxc1a

Plasmid

#132971

Purpose

full-length of foxc1a without stop codon in pmE Gateway vector

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Insert

foxc1a (foxc1a Zebrafish)

Use

Gateway vector

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pRD190

Plasmid

#163100

Purpose

Expression of mEos3.2 in eukaryotes to stain the nuclear compartment

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

NLS_mEos3.2_NLS

Use

Eukaryotic expression

Expression

Mammalian

Promoter

CMV promoter; T7 promoter

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-CS-154

Plasmid

#166230

Purpose

lenti-U6-gRNA::CMVp-nCas9-PmCDA1-UGI-2A-mCherry

Depositor

Nozomu Yachie

Article

Fukushima et al Sci Rep. 2021 Dec 13;11(1):23889. doi: 1

Inserts

U6-gRNA EGFP targetting
nCas9-PmCDA1-UGI-2A-mCherry

Use

CRISPR and Lentiviral

Promoter

CMV promoter and Human U6

Available Since

Dec. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-NLS/NES-mut

Plasmid

#42002

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

R930A, R931A, R932A, R934A, F951A, F955A, I958A, …

Promoter

CMV

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

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pPBpuro-mNG-eDHFR(69K6)-Tiam1

Plasmid

#172102

Purpose

Mammalian expression of Tiam1(DH-PH) fused to mNeonGreen-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

mNeonGreen-eDHFR(69K6)-Tiam1(DH-PH) (TIAM1 Human)

Expression

Mammalian

Promoter

CAG

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-3xHA-deltaCoiledCoil-LIN-41

Plasmid

#52720

Purpose

retroviral expression of human LIN-41 / TRIM71 containing deletion of the Coiled Coil domain and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

deleted amino acids 328–447

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-hFOXH1GR

Plasmid

#59311

Purpose

Forced expression of human FOXH1GR

Depositor

Shinya Yamanaka

Article

Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

Insert

FOXH1 (FOXH1 Human)

Use

Retroviral

Tags

cGR - hormone binding domain of glucocorticoid re…

Expression

Mammalian

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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