We narrowed to 7,494 results for: tet on
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Plasmid#161748PurposeTet-ON tTA plasmid for the c-terminal targeting of human GSX2 locusDepositorInsertAdvanced tTA
UseCRISPRExpressionMammalianPromoterendogenous GSX2 promoterAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIBA3-SpyCatcherEQ-sfGFP
Plasmid#107421PurposeFor production of inactive SpyCatcherEQ protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.DepositorInsertSpyCatcher
TagsHis tag, StrepII, and superfolder GFPExpressionBacterialMutationmutation of glutamate 77 to glutaminePromoterTetAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIBA2-SpyTag-MBP
Plasmid#107422PurposeControl construct for SpyCatcher experiments. Encodes MBP with N-terminal SpyTag, including OmpA signal peptide for periplasmic targeting.DepositorInsertMaltose-binding protein (MalE)
TagsOmpA signal peptide, SpyTag, and StrepIIExpressionBacterialPromoterTetAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFP-RIP2_S176A
Plasmid#131202Purposemammalian expression, tet RDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA-sfGFP
Plasmid#162689PurposepFA vector control expressing sfGFPDepositorInsertsfGFP
TagsC terminal 6x His tagExpressionBacterialPromoterPL tet promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRDA_103
Plasmid#133460PurposeH1 promoter with two Tet operator (TetO) sites expresses customizable Spyo-guide; short EF1a promoter (EFS) expresses SaurCas9, 2A provides TetR, and 2A provides blasticidin resistance.DepositorInsertcontrol guide
UseCRISPR and LentiviralExpressionMammalianAvailable SinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-pfkB
Plasmid#102284PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting pfkB.DepositorInsertpfkB gRNA
UseCRISPRExpressionBacterialPromoterpTetAvailable SinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS2_HindIII
Plasmid#65666PurposeFLAG-HA expression vector with RBPMS2 carrying mutation to introduce HindIII siteDepositorInsertRBPMS2 (RBPMS2 Human)
TagsFLAG HAExpressionMammalianMutationintroduced HindIII site (final goal to exchange F…PromoterCMV, 2x TetAvailable SinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVBL0001
Plasmid#242429PurposeStable genomic integration of Opto-IRE1 (HsIRE1dLD-mCherry-CRY2clust) using the Flp-in system.DepositorInsertIRE1alpha (ERN1 Human)
TagsmCherry, CRY2clustExpressionMammalianMutationDeletion of the lumenal domainPromoterCMV, tet-inducibleAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLVUT-spC3GFP
Plasmid#225487PurposeTet-on lentiviral vector for controlled expression of a secretable and permeable form of C. botulinum exoenzyme C3 transferase fused with EGFPDepositorInsertSecretable and permeable exoenzyme C3 transferase
UseLentiviralTagsEGFPExpressionMammalianMutationAmino acids 30-251PromoterHuman ubiquitin CAvailable SinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO–γ-tubulinWT-RNAi-resistant-GFP
Plasmid#205603PurposeTet-inducible expression of WT gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertionDepositorInsertγ-tubulinWT-RNAi-resistant-GFP
TagsGFPExpressionMammalianMutationG2228A, C2231T, C2237T, G2240AAvailable SinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-TAB-mOvol1
Plasmid#120360PurposepiggyBac transposon for dox-inducible expression of Ovol1 (with Bgeo; ie. LacZ / NeoR fusion).DepositorAvailable SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
DT047A
Plasmid#159408PurposeConstitutive LuxR expression, controlled under T7 bacteriophage promoter, and inducible PFO-sfGFP chimeric protein expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoterDepositorInsertpr.T7-LuxR-pr.LuxR-sfGFP-PFO
UseSynthetic BiologyTagsFLAG/FLAGExpressionBacterialPromotertet and pLux promoterAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFP-RIP2_S176E
Plasmid#131203Purposemammalian expression, tet RDepositorAvailable SinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFP-RIP2_3K
Plasmid#131205Purposemammalian expression, tet RDepositorInsertRIPK2 K209/410/538R (RIPK2 Human)
TagsEGFPExpressionMammalianMutationK209/410/538RPromoterpCMVAvailable SinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFP-RIP2_Y474F
Plasmid#131204Purposemammalian expression, tet RDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP M1 S245X
Plasmid#92372PurposeInducible expression of M1S245X spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
ExpressionMammalianMutation1-194 bp of 5'UTR deleted, S245XPromoterTet-responsive P tightAvailable SinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP M1 N184X
Plasmid#92371PurposeInducible expression of M1 N184X spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
ExpressionMammalianMutation1-194 bp of 5'UTR deleted, N184XPromoterTet-responsive P tightAvailable SinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR
Plasmid#65091PurposeDestination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) for stable cell line generationDepositorInsertRBPMS (RBPMS Human)
TagsFLAG HAExpressionMammalianMutationchanged sequence to render plasmid resistant to s…PromoterCMV, 2x TetAvailable SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestMYC_RBPMS_KDR
Plasmid#65667PurposeMYC expression vector with RBPMS encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
TagsMYCExpressionMammalianMutationintroduced HindIII site to exchange FLAG-HA tag w…PromoterCMV, 2x TetAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only