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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 2841 - 2860 of 16383 results

  1. pGTM_COV2_NSP5_C145A_001_SUMO

    Plasmid
    #192349
    Purpose
    Expresses SARS-CoV-2 3CLpro with an active site mutation (C154A) for the expression of the mature protease in an inactive form
    Depositor
    Gaetano Montelione
    Article
    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi
    Insert
    SARS-COV-2 Mpro-C145A (NEWENTRY SARS-CoV-2)
    Tags
    6X-His-Sumo
    Expression
    Bacterial
    Mutation
    Cysteine 145 of the active site is substituted wi…
    Promoter
    T7 lac
    Available Since
    June 3, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    192349-plasmid-map-sequence-id-381344

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  6. pcDNA3.1-MF3958 Heavy chain-SpyTag003

    Plasmid
    #216303
    Purpose
    Expresses anti-HER2 MF3958 Heavy chain-SpyTag003 in mammalian cells. To be paired with MF3958 light chain to form the anti-HER2 MF3958 Fab
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    MF3958 Heavy chain-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 tag, Signal peptide, and SpyTag003
    Expression
    Mammalian
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216303-plasmid-map-sequence-id-433368

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  8. pcDNA3.1-Tras heavy chain-SpyTag003

    Plasmid
    #216293
    Purpose
    Expresses anti-HER2 Tras Heavy chain-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras NoLink Fab
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    Tras heavy chain-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 tag, Signal peptide, and SpyTag003
    Expression
    Mammalian
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216293-plasmid-map-sequence-id-433208

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  9. pcDNA3.1-39S Heavy chain-SpyTag003

    Plasmid
    #216296
    Purpose
    Expresses anti-HER2 39S Heavy chain-SpyTag003 in mammalian cells. To be paired with 39S light chain to form the anti-HER2 39S Fab
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    39S Heavy chain-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 tag, Signal peptide, and SpyTag003
    Expression
    Mammalian
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216296-plasmid-map-sequence-id-433365

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  16. pBtPIPLC His

    Plasmid
    #173808
    Purpose
    Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells
    Depositor
    Anne Gershenson, Mary F Roberts
    Article
    He et al J Biol Chem. 2015 Jul 31;290(31):19334-4
    Insert
    Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
    Tags
    6XHis
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7
    Available Since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    173808-plasmid-map-sequence-id-345681

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  18. CC1215

    Bacterial Strain
    #79062
    Purpose
    BL21(DE3)-based E. coli purine auxotrophic host strain used for the preparation of PurE proteins in a purE background.
    Depositor
    T. J. Kappock
    Bacterial Resistance
    Kanamycin
    Available Since
    July 26, 2016
    Availability
    Academic Institutions and Nonprofits only

  20. mCit-APPAPAPPAP-gs

    Plasmid
    #228091
    Purpose
    Mammalian expression of 10 coiled coil segments in APPAPAPPAP arrangement, with short flexible linkers and N-terminal mCitrine for phase separation and forming of liquid condensates in mammalian cells
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, APPAPAPPAP
    Expression
    Mammalian
    Available Since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    228091-plasmid-map-sequence-id-451163

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Showing: 2841 - 2860 of 16383 results