We narrowed to 23,585 results for: lenti

1. pLenti- V6.3 lox-dsRED-stop-lox-eGFP-blas

   Plasmid

   #106171

   Purpose

   cre mediated red to green shift

   Depositor

   Ewa Snaar-Jagalska

   Article

   Heitzer et al Sci Rep. 2019 Mar 11;9(1):4096. doi: 10.

   Inserts

   dsRED-express2
   eGFP
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-Lenti-CAGGS-hPRDM6-2xGly-V5-EF1-Luc2-Blast

   Plasmid

   #213826

   Purpose

   Expresses human PRDM6 with a C-terminal V5 epitope tag.

   Depositor

   Bjoern Schwer

   Article

   Schmidt et al bioRxiv. 2023 Aug 31:2023.08.29.555389.

   Insert

   PRDM6 (PRDM6 Human)
   

   Use

   Lentiviral and Luciferase

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Feb. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. U6-DNMT1-hSynapsin-PE2-Nterminal-P2A-EGFP-KASH-lenti

   Plasmid

   #135955

   Purpose

   Neuronal expression of N-terminal intein-split prime editor v2 (PE2) with EGFP-KASH marker and U6-mDNMT1 cassette. This plasmid is used by PE2, PE3, and PE3b

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   Prime editor v2 (PE2) N-terminal
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hSynapsin

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiRNACRISPR_001 - hU6-DR_BsmBI-EFS-PguCas13b-NLS-2A-Puro-WPRE

   Plasmid

   #138143

   Purpose

   Expresses PguCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   PguCas13b
   

   Use

   Lentiviral

   Tags

   NLS

   Available Since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. tre-stblHIF: pLenti-TRE-stblHIF-PGK-rtTAA-2A-Puro

   Plasmid

   #177203

   Purpose

   encoding stblHIF under inducible promoter

   Depositor

   Markus Covert

   Article

   Kudo et al bioRxiv (2022)

   Insert

   stblHIF
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   P402A/P577G/N813A

   Promoter

   TRE

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiRNACRISPR_003 - hU6-DR_BsmBI-EFS-PspCas13b-NLS-2A-Puro-WPRE

   Plasmid

   #138145

   Purpose

   Expresses PspCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI.

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   PspCas13b
   

   Use

   Lentiviral

   Tags

   NLS

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiRNACRISPR_004 - hU6-DR_BsmBI-EFS-PspCas13b-NES-2A-Puro-WPRE

   Plasmid

   #138146

   Purpose

   Expresses PspCas13b in mammalian cells, localized in the cytoplasm. For cloning guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang acac. R overhang caac

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   PspCas13b
   

   Use

   Lentiviral

   Tags

   NES

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide1

   Plasmid

   #118162

   Purpose

   CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the EGFP CDS

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   hPGK and U6

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. JDW 1475 (pLenti-BLAST-GATA4-P2A-H2A-mCherry)

   Plasmid

   #233549

   Purpose

   A CMV driven human GATA4 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   GATA4 (GATA4 Human)
   

   Use

   Lentiviral

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide2

    Plasmid

    #118163

    Purpose

    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA2 against the EGFP CDS

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    hPGK and U6

    Available Since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide3

    Plasmid

    #118164

    Purpose

    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA3 against the EGFP CDS

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    hPGK and U6

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid

    #118158

    Purpose

    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDS

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1a core and U6

    Available Since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid

    Plasmid

    #154429

    Purpose

    hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid

    Depositor

    David Liu

    Article

    Koblan et al Nature. 2021 Jan;589(7843):608-614. doi:

    Insert

    hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    VRQR point mutations in SpCas9

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLENTI-CMV-GFP-TREX1 delta C (aa1-235) BLAST

    Plasmid

    #164229

    Purpose

    lentiviral plasmid for GFP-hTREX1 delta C (aa1-235)

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 235-314

    Promoter

    CMV

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCA26-pLenti.CAG.-NLS-dCas13b-NLS-VPR-T2A-HaloTag

    Plasmid

    #199457

    Purpose

    Expression of dCas13-VPR in mammalian cells

    Depositor

    Baohui Chen

    Article

    Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

    Insert

    dCas13
    

    Use

    Lentiviral

    Tags

    VPR-T2A-HaloTag

    Expression

    Mammalian

    Mutation

    Nuclease-dead H133A and H1058A

    Promoter

    CAG

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLENTI CMV GFP-TREX1 delta N (aa236-314) BLAST

    Plasmid

    #164235

    Purpose

    lentiviral plasmid for GFP-TREX1 delta N (aa236-314)

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deletion of amino acids 1-234

    Promoter

    CMV

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiRNACRISPR_002 - hU6-DR_BsmBI-EFS-PguCas13b-NES-2A-Puro-WPRE

    Plasmid

    #138144

    Purpose

    Expresses PguCas13b in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    PguCas13b
    

    Use

    Lentiviral

    Tags

    NES

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAR010 Lenti pEF-dEnAsCas12a-NLS-3XHA-T2A-BSD

    Plasmid

    #195543

    Purpose

    dCas12a effector fusion for manipulating transcription

    Depositor

    Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dEnAsCas12a-NLS-3XHA-T2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1

    Plasmid

    #118157

    Purpose

    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDS

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-P2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1a core and U6

    Available Since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-CMV-Pink Flamindo-p2a-paGFP-bPAC-CAAX

    Plasmid

    #202718

    Purpose

    Mammalian co-expression of soluble Pink Flamindo and membrane-bound bPAC for cAMP perturbation and imaging

    Depositor

    Adam Cohen

    Article

    Xiang et al bioRxiv 2023

    Inserts

    Pink Flamindo
    paGFP-bPAC-CAAX
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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