We narrowed to 11,747 results for: SHA

1. IR25a-T2A-QF-AD

   Plasmid

   #162526

   Purpose

   Plasmid for CRISPR-mediated knock-in line that expresses the activation domain of QF by knocking-in a T2A-QF-AD fragment replacing the stop codon of the endogenous Aedes aegypti Ir25a gene

   Depositor

   Leslie Vosshall

   Article

   Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

   Insert

   Ir25a-left-HDR-arm; T2A-QF2AD-Zip+-SV40; 3xP3-EYFP-SV40; Ir25a-right-HDR-arm
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. ORCO-T2A-QF-DBD

   Plasmid

   #162525

   Purpose

   Plasmid for CRISPR-mediated knock-in line that expresses the DNA-binding domain of QF by knocking-in a T2A-QF-DBD fragment replacing the stop codon of the endogenous Aedes aegypti Orco gene

   Depositor

   Leslie Vosshall

   Article

   Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

   Insert

   Orco-left-HDR-arm; T2A-Zip-::QFDBD-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.2-Cx43-HA

   Plasmid

   #49851

   Purpose

   Encodes human connexin 43 with a C-terminal HA epitope fusion tag

   Depositor

   Robin Shaw

   Article

   Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

   Insert

   Connexin 43 (GJA1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Contains several wobble mutations to confer resis…

   Promoter

   CMV

   Available since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCIP-Flag-TfebAA

   Plasmid

   #79014

   Purpose

   For mammalian expression of a Flag-tagged, constitutively active version (S142A, S211A) of Tfeb.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   Tfeb (Tfeb Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S142A, S211A

   Promoter

   pCAGGs

   Available since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. DPYSL2-pLJC2

   Plasmid

   #189866

   Purpose

   Expresses human DPYSL2 (CRMP2) in mammalian cells

   Depositor

   Yoav Shaul

   Article

   Abu Rmaileh et al J Cell Biol. 2022 Jul 4;221(7). pii: 213

   Insert

   DPYSL2 (DPYSL2 Human)
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNCST-AausFP1

   Plasmid

   #129499

   Purpose

   Expresses AausFP1 constitutively in E. coli (most strains)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   AausFP1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   synthetic constitutive (stationary phase) promote…

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. IMPT-10314:GABBR2

   Plasmid

   #157851

   Purpose

   HA-3xFLAG-VFT-TMD-CC in pFastBac1 for Sf9 expression

   Depositor

   Raymond Stevens, Vadim Cherezov

   Article

   Shaye et al Nature. 2020 Jun 17. pii: 10.1038/s41586

   Insert

   GABBR2 (GABBR2 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMVd1-NLS-GAL4-iLight2-VP16-T2A-mTagBFP2

   Plasmid

   #219548

   Purpose

   NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

   Depositor

   Vladislav Verkhusha

   Article

   Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

   Insert

   NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The (SAGG)x4 linker between GAL4(1-65) and the PC…

   Promoter

   CMVd1

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST/hCx43-EGFP-N1

   Plasmid

   #40907

   Depositor

   Robin Shaw

   Article

   Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

   Insert

   Connexin 43 (GJA1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   No stop codon to create c-terminal fusion

   Promoter

   CMV

   Available since

   Nov. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cas9-GFP_sg_mTfeb

    Plasmid

    #79006

    Purpose

    Expresses WT Cas9 and GFP, along with a sgRNA to mouse Tfeb.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    sgTfeb (Tfeb Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. EXT1-pLJC2-3XFLAG

    Plasmid

    #215818

    Purpose

    Ectopic expression of EXT-1 in mammalian cells

    Depositor

    Yoav Shaul

    Article

    Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

    Insert

    Exostosin glycosyltransferase 1 (EXT1 Human)
    

    Use

    Lentiviral

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVH-EF1a-GFP-P2A-MYC

    Plasmid

    #130695

    Purpose

    Lentiviral plasmids encoding human MYC with co-expressoin of GFP mediated by P2A.

    Depositor

    Kejin Hu

    Article

    Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

    Insert

    human MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-Puro-sgNUAK1

    Plasmid

    #138692

    Purpose

    Expresses a human NUAK1-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgNUAK1 human (NUAK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCSII-EF-miRFP670v1-hGem(1/110)

    Plasmid

    #80006

    Purpose

    hGem(1/110) fused with near-infrared fluorescent protein miRFP670v1 (S/G2/M phase cell cycle reporter)

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    hGeminin (1/110) (GMNN Human)
    

    Use

    Lentiviral

    Tags

    miRFP670v1

    Expression

    Mutation

    Promoter

    EF-1 alpha

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCEP4-myc-HLA-A24

    Plasmid

    #135507

    Purpose

    Mammalian expression of myc-tagged HLA-A*024:02

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*24:02 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-Puro-sgNUAK2

    Plasmid

    #138693

    Purpose

    Expresses a human NUAK2-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgNUAK2 human (NUAK2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. PtPuc3_diaCas9_sgRNA

    Plasmid

    #109219

    Purpose

    Episome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutum

    Depositor

    Per Winge

    Article

    Sharma et al Sci Rep. 2018 Sep 26;8(1):14401. doi: 10

    Inserts

    LHCF2 promoter
    diaCas9
    LHCF1 terminator
    U6 promoter
    sgRNA
    U6 3' region
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    LHCF1 terminator, LHCF2 promoter, U6 3' regi…

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVH-EF1a-GFP-P2A-SOX2

    Plasmid

    #130693

    Purpose

    Lentiviral plasmids encoding human SOX2 with co-expressoin of GFP mediated by P2A.

    Depositor

    Kejin Hu

    Article

    Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

    Insert

    human SOX2 (SOX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL4.23_Promoter_1-3

    Plasmid

    #210629

    Purpose

    Luciferase experiment for TAL1 promoter 1-3

    Depositor

    Maayan Salton

    Article

    Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

    Insert

    promoter 1-3 (TAL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal Promoter

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCSII-EF-miRFP709-hCdt(1/100)

    Plasmid

    #80007

    Purpose

    hCdt(1/100) fused with near-infrared fluorescent protein miRFP709 (G1 phase cell cycle reporter)

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    hCdt1(1/100) (CDT1 Human)
    

    Use

    Lentiviral

    Tags

    miRFP709

    Expression

    Mutation

    Promoter

    EF-1 alpha

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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