We narrowed to 59,421 results for: cloning

1. pG108-K - AhpC-Glow

   Plasmid

   #185135

   Purpose

   Bacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers BS2 is cloned under the Bacteroides thetaiotaomicron ahpC promoter

   Depositor

   Janina Lewis

   Article

   Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

   Inserts

   Glow gene
   Bacteroides thetaiotaomicron ahpC promoter
   

   Use

   Pg106

   Expression

   Bacterial

   Available Since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. STAGR_gRNAScaffold_hU6

   Plasmid

   #102840

   Purpose

   Can be used as PCR template for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Insert

   gRNAScaffold_hU6promoter
   

   Promoter

   hU6

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEMT-PT2A-GFP-Tdtomato-iRFP670

   Plasmid

   #111812

   Purpose

   Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Inserts

   GFP
   Tdtomato
   iRFP670
   

   Use

   Cloning intermediate

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMOD_C3001

   Plasmid

   #91094

   Purpose

   Module C, Promoter: 35S, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   GFP (+ BaeI site for GT donor cloning)
   

   Use

   Unspecified

   Promoter

   35S

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3E-FRT-KanR-FRT pA

   Plasmid

   #82601

   Purpose

   3' entry vector containing FRT-flanked kanamycin resistance cassette (KanR) with pA for FLP-induced kanamycin resistance, drug selection

   Depositor

   Kryn Stankunas

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   FRT-Kan-FRT
   

   Promoter

   None

   Available Since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAC-ZEAX

   Plasmid

   #53274

   Purpose

   Contains crtE, crtB, crtI, crtY and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces zeaxanthin in E. coli

   Depositor

   Francis X Cunningham Jr

   Article

   Sun et al J Biol Chem. 1996 Oct 4;271(40):24349-52

   Insert

   crtE, crtY, crtI, crtB, crtZ
   

   Use

   Low copy number bacterial cloning vector

   Mutation

   Deleted idi (ORF6) and crtX genes

   Promoter

   endogenous promoters

   Available Since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR/zeo-MACROD1

   Plasmid

   #172572

   Purpose

   For gateway cloning of N-terminal tagged MACROD1 constructs.

   Depositor

   Karla Feijs

   Article

   Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

   Insert

   MACROD1 (MACROD1 Human)
   

   Use

   Gateway cloning

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR/zeo-MACROD2

   Plasmid

   #172573

   Purpose

   For gateway cloning of N-terminal tagged MACROD2 constructs.

   Depositor

   Karla Feijs

   Article

   Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

   Insert

   MACROD2 (MACROD2 Human)
   

   Use

   Gateway cloning

   Available Since

   April 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR221-NR1H3

   Plasmid

   #79514

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H3 CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   LXR Alpha (NR1H3 Human)
   

   Use

   Multisite gateway entry clone

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAT9679-sgRNA-BFP

    Plasmid

    #162988

    Purpose

    sgRNA cloning plasmid

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    exchangeable cassette
    

    Use

    CRISPR

    Expression

    Bacterial and Mammalian

    Promoter

    U6

    Available Since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXTK066 [Con-Pro_nptII-Broad_PartType8]

    Plasmid

    #229303

    Purpose

    XanthoMoClo Golden Gate Part Plasmid for cloning, contains Constitutive-Promoter_nptII-Broad_PartType8

    Depositor

    Michael Springer

    Article

    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

    Insert

    nptII Promoter (Broad)
    

    Expression

    Bacterial

    Available Since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223 LC3C WT

    Plasmid

    #123078

    Purpose

    Gateway entry clone encoding human MAP1LC3C wild-type

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
    

    Use

    Gateway entry vector / entry clone

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. B52_puro_empty_gRNA

    Plasmid

    #197557

    Purpose

    sites for cloning two gRNA with puromycin cassette

    Depositor

    Darius Bägli

    Type

    Empty backbone

    Use

    Empty grna backbone with puromycin resistance gene

    Expression

    Mammalian

    Promoter

    U6 for gRNA, CMV for puromycin resistance.

    Available Since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMOD_C3003

    Plasmid

    #91095

    Purpose

    Module C, Promoter: CmYLCV, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    GFP (+ BaeI site for GT donor cloning)
    

    Use

    Unspecified

    Promoter

    CmYLCV

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. IZ501: pMVP/Lenti-DEST

    Plasmid

    #121848

    Purpose

    pMVP destination vector, empty Lentivirus vector backbone

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Lentiviral; Pmvp gateway destination vector

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAT9658-sgRNA-mCherry

    Plasmid

    #162987

    Purpose

    sgRNA cloning plasmid

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    exchangeable cassette
    

    Use

    CRISPR

    Expression

    Bacterial and Mammalian

    Promoter

    U6

    Available Since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. STAGR_SAMScaffold_hU6

    Plasmid

    #102843

    Purpose

    Can be used as PCR template for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Insert

    gRNAScaffold_hU6promoter
    

    Use

    As pcr template

    Available Since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR221-NFAT5

    Plasmid

    #79506

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NFAT5 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    NFAT5 (NFAT5 Human)
    

    Use

    Multisite gateway entry clone

    Promoter

    no Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEMT-PT2A-iRFP670-Tdtomato-GFP

    Plasmid

    #111817

    Purpose

    Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Inserts

    iRFP670
    Tdtomato
    GFP
    

    Use

    Cloning intermediate

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSUMO-YHRC

    Plasmid

    #54336

    Purpose

    Expression vector (pT7) for untagged expression as well as fusions to His6-SUMO or His6 tags. Designed for single or multiple fragment cloning by yeast homologous recombination.

    Depositor

    Claes Andréasson

    Article

    Holmberg et al Protein Expr Purif. 2014 Jun;98:38-45. d

    Type

    Empty backbone

    Tags

    6His-SUMO (yeast gene: SMT3)

    Expression

    Bacterial

    Promoter

    T7-LacO

    Available Since

    Aug. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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