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pGL4.23 C3_17

Plasmid

#60300

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

ZFAND3 enhancer (ZFAND3 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGL4.23 C3_18

Plasmid

#60301

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

ZBED3 enhancer (ZBED3 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGL4.23 C3_22

Plasmid

#60304

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

ZMIZ1 enhancer (ZMIZ1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pENTR D-TOPO-C2_8

Plasmid

#60249

Purpose

This plasmid contains a human pancreatic islet inactive enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

RAP1A inactive enhancer (RAP1A Human)

Use

Gateway Cloning

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR D-TOPO-C2_11

Plasmid

#60251

Purpose

This plasmid contains a human pancreatic islet inactive enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

SMYD3 inactive enhancer (SMYD3 Human)

Use

Gateway Cloning

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR D-TOPO-C2_13

Plasmid

#60252

Purpose

This plasmid contains a human pancreatic islet inactive enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

SPATA19 inactive enhancer (SPATA19 Human)

Use

Gateway Cloning

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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PGKp-GFP-TAZ

Plasmid

#174176

Purpose

Lentiviral vector to constitutively express TAZ/WWTR1 under control of the human PGK promoter.

Depositor

Rod Bremner

Article

Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

Insert

TAZ (WWTR1 Human)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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PGKp-GFP-YAP

Plasmid

#174172

Purpose

Lentiviral vector to constitutively express YAP under control of the human PGK promoter.

Depositor

Rod Bremner

Article

Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

Insert

YAP (YAP1 Human)

Use

Lentiviral

Tags

3x FLAG tag

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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PGKp-GFP-YAP (S94A)

Plasmid

#174173

Purpose

Lentiviral vector to constitutively express TEAD-binding mutant YAP under control of the human PGK promoter.

Depositor

Rod Bremner

Article

Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

Insert

YAP (YAP1 Human)

Use

Lentiviral

Tags

3x FLAG tag

Expression

Mammalian

Mutation

Serine 94 mutated to Alanine

Promoter

PGK

Available Since

Aug. 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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JDW 742 (pDONR221-EGFP-KRAS4B-G12V)

Plasmid

#156403

Purpose

Gateway middle entry clone encoding EGFP-fused to human KRAS4B with G12V mutation, includes a stop codon

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4B-G12V (KRAS Human)

Use

Gateway Cloning

Tags

EGFP

Mutation

mutant KRAS4B-G12V

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pPHR-mCherry-hNPM

Plasmid

#181887

Purpose

Soluble light-dependent effector protein (nucleophosmin) for optodroplet formation or recruitment to CIBN localizers

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

PHR-mCherry-hNPM (NPM1 Human, Mustard Weed)

Tags

PHR-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pPHR-mCherry-hHP1alpha

Plasmid

#181884

Purpose

Soluble light-dependent effector protein (human HP1alpha) for optodroplet formation or recruitment to CIBN localizers

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

PHR-mCherry-hHP1alpha (CBX5 Human, Mustard Weed)

Tags

PHR-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3.3_MERS_D12

Plasmid

#170448

Purpose

Express MERS WT spike protein with an 12aa deletion on the C-terminal tail. Used for MERS pseudotyped virus production.

Depositor

David Nemazee

Article

Rogers et al Science. 2020 Aug 21;369(6506):956-963.

Insert

MERS spike D12 (S MERS)

Expression

Mammalian

Mutation

last 12aa deletion in c-terminal tail

Promoter

CMV

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-TRE3G-hYAP1-CMV-mCherry-T2A-Puro

Plasmid

#176850

Purpose

lentiviral vector for doxycycline-inducible YAP1 overexpression

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

hYAP1 (YAP1 Human)

Use

Lentiviral

Mutation

full length YAP1

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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EFSp-GFP-YAP (S94A)

Plasmid

#174169

Purpose

Lentiviral vector to constitutively express TEAD-binding mutant YAP under control of the EFS promoter.

Depositor

Rod Bremner

Article

Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

Insert

YAP (YAP1 Human)

Use

Lentiviral

Tags

3x FLAG tag

Expression

Mammalian

Mutation

Serine 94 mutated to Alanine

Promoter

EFS

Available Since

Aug. 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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EFSp-GFP-YAP

Plasmid

#174168

Purpose

Lentiviral vector to constitutively express YAP under control of the EFS promoter.

Depositor

Rod Bremner

Article

Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

Insert

YAP (YAP1 Human)

Use

Lentiviral

Tags

3x FLAG tag

Expression

Mammalian

Promoter

EFS

Available Since

Aug. 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pLV-TRE3G-YAP1-5SA-CMV-mCherry-T2A-Puro

Plasmid

#176851

Purpose

lentiviral vector for doxycycline-inducible YAP1 5SA constitutively active expression

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

hYAP1-5SA (YAP1 Human)

Use

Lentiviral

Mutation

S61A, S109A, S127A, S164A, S381A (phosphorylation…

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-YAP1-shRNA1

Plasmid

#176846

Purpose

lentiviral vector for doxycycline-inducible human YAP1 knockdown

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

YAP1-Sh1 (YAP1 Human)

Use

Lentiviral

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-CRY2oligo-mcherry-ANXA11

Plasmid

#164203

Purpose

Expresses CRY2oligo-mcherry tagged ANXA11 under CMV promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Tags

CRY2oligo-mcherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_CoV1_D28

Plasmid

#170447

Purpose

Express SARS-CoV-1 WT spike protein with a 28aa deletion on the C-terminal tail. Used for SARS1 pseudotyped virus production.

Depositor

David Nemazee

Article

Rogers et al Science. 2020 Aug 21;369(6506):956-963.

Insert

SARS-CoV-1 spike D28 (S SARS-CoV-1)

Tags

NA

Expression

Mammalian

Mutation

last 28aa deletion in c-terminal tail

Promoter

CMV

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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