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pJW1513

Plasmid

#62383

Purpose

pRS316-pTDH3-Su9-TagBFP

Depositor

Jonathan Weissman

Article

Williams et al Science. 2014 Nov 7;346(6210):748-51. do

Insert

su9-tagBFP

Tags

Su9 presequence

Expression

Yeast

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-ura4MX6

Plasmid

#49184

Purpose

gene deletion in S. pombe

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Expression

Yeast

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR207-MpPHOT

Plasmid

#100600

Purpose

Gateway entry vector for MpPHOTOTROPIN

Depositor

Yutaka Kodama

Article

Kodama et al PLoS One. 2016 Mar 30;11(3):e0152484. do

Insert

MpPHOT

Use

Gateway entry vector

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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sortase A pentamutant (eSrtA) in pET29

Plasmid

#75144

Purpose

evolved sortase A (eSrtA) pentamutant with improved kinetics and activity

Depositor

David Liu

Article

Chen et al Proc Natl Acad Sci U S A. 2011 Jul 12;10

Insert

sortase A pentamutant

Tags

6x His

Expression

Bacterial

Mutation

P94R, D160N, D165A, K190E, K196T. Deleted amino a…

Promoter

T7

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-Hygro-PIP-FUCCI

Plasmid

#138714

Purpose

Lentiviral expression of the cell cycle marker PIP-FUCCI

Depositor

Jean Cook

Article

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

PIP-FUCCI

Use

Lentiviral

Tags

mCherry and mVenus

Expression

Mammalian

Promoter

hPGK

Available Since

May 8, 2020

Availability

Academic Institutions and Nonprofits only

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pET21a-GlyRS-His

Plasmid

#124110

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Glycyl-tRNA ligase (glyS, glyQ Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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Galphai1-mTurquoise2

Plasmid

#69620

Purpose

The heterotrimeric G-protein subunit, Gαi isoform 1, tagged with mTurquoise2

Depositor

Dorus Gadella

Article

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Insert

Galphai1-mTurquoise2

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only
pBstSK+IRES-V5-IPpoI

Plasmid

#206321

Purpose

A plasmid that facilitates production of mRNA encoding V5 epitope tagged I-PpoI

Depositor

Brian McStay

Article

van Sluis et al Genes Dev. 2015 Jun 1;29(11):1151-63. do

Insert

IRES element-V5 epitope tag- IPpoI open reading frame

Use

Mrna production

Tags

V5-IPpoI fusion protein

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pC0.120

Plasmid

#119616

Purpose

Level 0 part. Linker

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

AbR Down Linker

Use

Synthetic Biology

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-Rab7Q67L

Plasmid

#169038

Purpose

Express human constitutively active form of Rab7a(Q67L) in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rab7a (RAB7A Human)

Tags

acGFP1

Expression

Mammalian

Mutation

Q67L

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pHR V5-RNF25_IRES-mCherry

Plasmid

#198386

Purpose

RNF25 expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RNF25 (RNF25 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1A

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-Neo-PIP-FUCCI

Plasmid

#118616

Purpose

Lentiviral expression of the cell cycle marker PIP-FUCCI

Depositor

Jean Cook

Article

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

PIP-FUCCI

Use

Lentiviral

Tags

mCherry and mVenus

Expression

Mammalian

Promoter

hPGK

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-F-RAM-d2tTA-TRE-mKate2

Plasmid

#140274

Purpose

F-RAM, the Fos-dependent reporter

Depositor

Yingxi Lin

Article

Sun et al Cell. 2020 Apr 16;181(2):410-423.e17. do

Insert

mKate2

Use

AAV

Promoter

TRE

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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pBITE-CD19-CD3

Plasmid

#190677

Purpose

Encodes a modularized form of HD37-OKT3 bispecific antibody

Depositor

Scott McComb

Article

Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

Insert

Modularized-HD37-modularized-OKT3-P2A-NeonGreen

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

EFS

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Cyto-V5-APEX2

Plasmid

#170572

Purpose

Expresses APEX2 in cytosol

Depositor

James Olzmann

Article

Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

Insert

APEX2

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Ki67-mNeonGreen-IRESpuro2

Plasmid

#183737

Purpose

Expresses Ki67-mNeonGreen

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67 (MKI67 Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Blast-PIP-FUCCI

Plasmid

#138715

Purpose

Lentiviral expression of the cell cycle marker PIP-FUCCI

Depositor

Jean Cook

Article

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

PIP-FUCCI

Use

Lentiviral

Tags

mCherry and mVenus

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-FRB

Plasmid

#25919

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

Insert

FRB (MTOR Human)

Expression

Mammalian

Available Since

Sept. 10, 2010

Availability

Academic Institutions and Nonprofits only

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pFA6a-FLAG-APEX2-NES-NatMX6

Plasmid

#229231

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Article

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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Frt-Atg-Hygro-CMV-green (mWasabi)-PA-F3

Plasmid

#67276

Purpose

RMCE donor plasmid for FlpO. the marker gene is green fluorescent (mWasabi) with PA

Depositor

Jannik Elverløv-Jakobsen

Article

Callesen et al PLoS One. 2016 Aug 19;11(8):e0161471. do

Inserts

GFP (mWasabi)
hygro

Tags

F3 and Frt

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2015

Availability

Academic Institutions and Nonprofits only

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