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pBF_HsPepT2

Plasmid

#73944

Purpose

Xenopus oocyte expression vector containing the cDNA of human PepT2 with a c terminal flag tag

Depositor

Simon Newstead

Publication

Beale et al Structure. 2015 Oct 6;23(10):1889-99. do

Insert

PepT2 (SLC15A2 Human)

Use

Make rna for xenopus oocyte injection

Tags

Flag

Promoter

beta globin

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only
HcRed-pcw107-V5

Plasmid

#64647

Purpose

(control) when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

n/a

Use

Lentiviral

Tags

V5

Promoter

PGK

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
HB101 endA::frt

Bacterial strain

#45473

Depositor

Michele Swanson

Publication

Bryan et al Mol Microbiol. 2011 Apr;80(1):231-47. do

Bacterial resistance

Streptomycin

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
Cbh_v5 AAV-saCBE N-terminal

Plasmid

#137182

Purpose

AAV genome: expresses the N-terminal of S. aureus v5 AAV-CBE from the Cbh promoter, U6-sgRNA

Depositor

David Liu

Publication

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-saCBE N-terminal

Use

AAV

Mutation

Cas9 D10A

Promoter

Cbh

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV.hSynapsin.SF-Venus-iGluSnFR.A184S

Plasmid

#106177

Purpose

Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-Venus-iGluSnFR.A184S

Use

AAV

Mutation

SFGFP: T203Y, F46L, T65G, S72A GltI: A184S

Promoter

hSynapsin

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pF CAG luc IRES neo

Plasmid

#98294

Purpose

For constitutive expression of firefly luciferase from the CAG promoter together with an IRES-linked neo resistance gene

Depositor

Brett Stringer

Publication

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Inserts

Firefly luciferase
IRES
Neomycin resistance gene (NeoR)

Use

Lentiviral and Luciferase

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAPK7_WT

Plasmid

#82925

Purpose

Gateway Donor vector containing MAPK7, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

NM_002749.3 (MAPK7 Human)

Use

Gateway Cloning

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFB2G

Plasmid

#195290

Purpose

Bicistronic HSV-TK and mTagBFP resistance for positive/negative selection

Depositor

Scott Lowe

Publication

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HSV-TK 2A mTagBFP

Expression

Mammalian

Promoter

PGK

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgRNA-EF1α-sakkhCas9-HA-NLS-polyA-CBh-EGFP-polyA

Plasmid

#168305

Purpose

mediated knock-in of sgRNA precursor

Depositor

Hui Yang

Publication

Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

Insert

SaKKH-EGFP-U6-sgRNA

Use

AAV

Expression

Mammalian

Promoter

EF1alpha, CBh

Available since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX307-IBV-3CL

Plasmid

#160288

Purpose

Allows for constituitive mammalian expression of the IBV 3CL protease via transfection or lentivirus

Depositor

Alejandro Chavez

Publication

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX307-Bat-CoV-HKU9-3CL

Plasmid

#160284

Purpose

Allows for constituitive mammalian expression of the Bat-CoV-HKU9 3CL protease via transfection or lentivirus

Depositor

Alejandro Chavez

Publication

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLX303-PCDH7-a

Plasmid

#108538

Purpose

Expresses human PCDH7 transcript variant a in mammalian cells

Depositor

Kathryn O’Donnell

Publication

Zhou et al Cancer Res. 2017 Jan 1;77(1):187-197. do

Insert

PCDH7 transcript variant a (PCDH7 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pKK-TEV-3XFLAG

Plasmid

#105784

Purpose

Expression of your protein of interest in fusion with 3xFLAG at the C-terminus. The tag is cleavable by TEV protease.

Depositor

Andrzej Dziembowski

Publication

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-3XFLAG

Expression

Mammalian

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Isl1

Plasmid

#72696

Purpose

Doxycyline regulated expression of Isl1 in mammalian cells

Depositor

Timothy Kamp

Publication

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Islet1 (Isl1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET30a(+)-His-SUMO-Tau2N4R

Plasmid

#257333

Purpose

For bacterial expression of Tau2N4R

Depositor

Maciej Walczak

Publication

Jing et al STAR Protoc. 2026 Jun 16;7(3):104617. do

Insert

Tau2N4R

Tags

6xHis-SUMO

Expression

Bacterial

Promoter

T7

Available since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-myo1e

Plasmid

#248918

Purpose

Expresses EGFP-tagged human myosin 1e in mammalian cells

Depositor

Mira Krendel, Mark Mooseker

Publication

Krendel et al FEBS Lett. 2007 Feb 20;581(4):644-50. do

Insert

Myo1e (MYO1E Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pHREP-LP

Plasmid

#240432

Purpose

Empty landing pad vector with two Bxb1 recombination sites for RMCE and LNGFR + EGFP-HygroR for sorting/selection.

Depositor

Lienhard Schmitz

Publication

Pfisterer et al PLoS One. 2026 Feb 10;21(2):e0342014. do

Inserts

LNGFR
EGFP

Tags

Hygromycin Resistance

Expression

Mammalian

Promoter

CMV

Available since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pHREP-KD-2

Plasmid

#240441

Purpose

Sleeping Beauty vector for inducible expression of an shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin.

Depositor

Lienhard Schmitz

Publication

Pfisterer et al PLoS One. 2026 Feb 10;21(2):e0342014. do

Type

Empty backbone

Expression

Mammalian

Available since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pVP077

Plasmid

#222029

Purpose

Empty Kanamycin resistant GreenGate destination vector based on pGGP-AG. Domesticated of Esp3I sites in the backbone and chloramphenicol resistance gene.

Depositor

Wayne Parrott

Publication

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All Esp3i sites domesticated

Available since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pGB

Plasmid

#204744

Purpose

gRNA-Bsd expression vector

Depositor

Yuichiro Miyaoka

Publication

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Type

Empty backbone

Expression

Mammalian

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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