We narrowed to 7,306 results for: GFP expression plasmids

1. pCFJ150-GFP(dpiRNA)::CDK-1

   Plasmid

   #107938

   Purpose

   cdk-1p::GFP(dpiRNA)::cdk-1::cdk-1 3'UTR construct used for MosSCI in nematode. GFP is optimized by removing all piRNA targeting sites to allow germline expression.

   Depositor

   Heng-Chi Lee

   Article

   Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

   Insert

   GFP(dpiRNA)::CDK-1 (cdk-1 Nematode)
   

   Expression

   Worm

   Promoter

   cdk-1

   Available Since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-HCMVgO-sfGFP (Merlin)

   Plasmid

   #136452

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein O (Merlin strain) fused to superfolder GFP

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   glycoprotein O (UL74 Human betaherpesvirus 5)
   

   Tags

   Gly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCALNL-tdTomato-2A-mSyp::EGFP

   Plasmid

   #191213

   Purpose

   Expresses tdTomato protein and mouse Synaptophysin protein fused with EGFP in Cre expressing mammalian cells.

   Depositor

   Theofanis Karayannis

   Article

   Gesuita et al STAR Protoc. 2022 Nov 11;3(4):101837. do

   Insert

   tdTomato-T2A-mSyp::EGFP (Syp Mouse)
   

   Use

   Pcagen

   Tags

   mSyp::EGFP

   Expression

   Mammalian

   Available Since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. CAG-GFP-IRES-CRE

   Plasmid

   #48201

   Purpose

   retroviral vector encoding for GFP and Cre-recombinase

   Depositor

   Fred Gage

   Article

   Zhao et al J Neurosci. 2006 Jan 4. 26(1):3-11.

   Inserts

   eGFP
   Cre
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   CAG and linked by IRES

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. N8his-GFPenhancer-GGGGS4-LaG16

   Plasmid

   #140442

   Purpose

   We designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.

   Depositor

   Motoyuki Hattori

   Article

   Zhang et al Sci Rep. 2020 Apr 10;10(1):6239. doi: 10

   Insert

   GFPenhancer-GGGGS4-LaG16
   

   Tags

   8 histidine tag

   Expression

   Bacterial

   Promoter

   T7 promotor

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. scFv-GCN4-sfGFP-TET1CD

   Plasmid

   #184439

   Purpose

   TET1 catalytic domain fused with sfGFP and scFv against GCN4

   Depositor

   Rhys Allan

   Article

   Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

   Insert

   scFv-GCN4-sfGFP-TET1CD
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. acta1-mRuby2-VHH[GFP]

   Plasmid

   #163856

   Purpose

   Zebrafish muscle specific expression of mRuby2-VHH[GFP]. Parton lab clone FRX

   Depositor

   Rob Parton

   Article

   Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

   Insert

   VHH[GFP]
   

   Use

   In vitro transcription of rna

   Tags

   Ruby2

   Available Since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB_TRE3G_scFV-sfGFP-Ring1bCD-CatMut

   Plasmid

   #235583

   Purpose

   Dox-inducible expression of control catalytic-mutant Ring1b CD fused with scFV

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   Ring1b (Rnf2 Mouse)
   

   Use

   CRISPR

   Mutation

   I53S

   Available Since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMMP-LMP1-IRES-eGFP

   Plasmid

   #36955

   Depositor

   Bill Sugden

   Article

   Pratt et al J Virol. 2012 Apr;86(8):4380-93. Epub 20

   Insert

   LMP1 (LMP-1 )
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-N1-mCherry-ApoB-TagBFP

    Plasmid

    #129383

    Purpose

    Plasmid bearing the editing target of APOBEC1 fused between the mCherry and the Tag-BFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of Tag-BFP

    Depositor

    Silvestro Conticello

    Article

    Chieca et al Methods Mol Biol. 2021;2181:69-81. doi:

    Insert

    mCherry-ApoB-Tag-BFP
    

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3.0_nucLAMA-G97 IRES EGFP

    Plasmid

    #136620

    Purpose

    Plasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    SNAP-Tag-LAMA-G97-NLS (IRES EGFP)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW-GFP-CtIP-T847E

    Plasmid

    #71111

    Purpose

    mammalian expession of GFP-CtlP T847E mutation

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    CtlP
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    T847E

    Promoter

    TRE promoter, Tet ON

    Available Since

    Dec. 9, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pCDNA3.0_nucLAMA-F98 IRES EGFP

    Plasmid

    #136618

    Purpose

    Plasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    SNAP-Tag-LAMA-F98-NLS (IRES EGFP)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. SREC 2: abu-1::abu-1::gfp

    Plasmid

    #21825

    Depositor

    David Ron

    Article

    Urano et al J Cell Biol. 2002 Aug 19. 158(4):639-46.

    Insert

    abu-1 promoter and coding region (abu-1 Nematode)
    

    Tags

    GFP

    Expression

    Worm

    Available Since

    Oct. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB_TRE3G_scFV-sfGFP-Setd2CD-CatMut

    Plasmid

    #235588

    Purpose

    Dox-inducible expression of control catalytic-mutant Setd2 CD fused with scFV

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Setd2 (Setd2 Mouse)
    

    Use

    CRISPR

    Mutation

    R1599C

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-T7-TadCBEd-SpRY-P2A-EGFP (BKS428)

    Plasmid

    #223125

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd-SpRY with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadCBEd-SpRY-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpRY=D10A/A61R/L1111R/D1135L…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-SpCas9(KWRQLC)-P2A-EGFP (RAS2864)

    Plasmid

    #223087

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(KWRQLC)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(KWRQLC)=D1135K, S1136W, G1218R, E1219Q, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-SpCas9(LWKYQS)-P2A-EGFP (AHK34)

    Plasmid

    #223079

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(LWKYQS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(LWKYQS)=D1135L, S1136W, G1218K, E1219Y, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-T7-SpCas9(MRKCRS)-P2A-EGFP (RAS2821)

    Plasmid

    #223102

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRKCRS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(MRKCRS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(MRKCRS)=D1135M, S1136R, G1218K, E1219C, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-T7-SpCas9(SRQMRG)-P2A-EGFP (RAS3017)

    Plasmid

    #223103

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with SRQMRG amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(SRQMRG)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(SRQMRG)=D1135S, S1136R, G1218Q, E1219M, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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