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pMpGWB328

Plasmid

#68656

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pNLRP11major.MYC

Plasmid

#131196

Purpose

Mammalian expression of human NLRP11 188S

Depositor

Thomas Kufer

Article

Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

Insert

NLRP11 188S (NLRP11 Human)

Tags

myc

Expression

Mammalian

Mutation

188S

Promoter

pCMV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-FOXI3

Plasmid

#153128

Purpose

Expression of Flag tagged human FOXI3in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXI3 (FOXI3 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pUM31dao

Plasmid

#87242

Purpose

Plastid expression cassette containing Schizosaccharomyces pombe D-amino acid oxidase gene. Provides dual positive (D-alanine) and negative (D-valine) selection in transplastomic plants

Depositor

Anil Day

Article

Gisby et al Plant Physiol. 2012 Dec;160(4):2219-26.

Insert

D-amino oxidase

Expression

Plant

Promoter

plastid rrn

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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Fast-GCaMP-EF05

Plasmid

#52558

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-EF05

Expression

Insect

Mutation

changed from GCaMP3: D397N/G398A/N399D

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pDH3

Plasmid

#83789

Purpose

Useful for tagging yeast proteins with C-terminal CFP tag; G418 selection

Depositor

Eric Muller

Article

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

CFP

Tags

CFP

Expression

Yeast

Mutation

F64L, Y66W, N146I, M153T, V163A, N164H mutations …

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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BII-ChPtW-PLAGL2

Plasmid

#133380

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

PLAGL2 (PLAGL2 Human)

Tags

HA

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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POM43-mCherry

Plasmid

#69857

Purpose

N-terminal tagging with mCherry using LEU2 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mCherry minus start and stop codon

Expression

Yeast

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-FOXN4

Plasmid

#153140

Purpose

Expression of Flag tagged human FOXN4 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXN4 (FOXN4 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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JC101: pMVP/Ad/PK-DEST

Plasmid

#121845

Purpose

pMVP destination vector, empty pK fiber-modified adenovirus vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Adenoviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-mWasabi-ER

Plasmid

#164165

Purpose

Expresses mWasabi fused with an ER-targeting sequence of calreticulin and the ER retrieval sequence, KDEL, under the control of CAG promoter

Depositor

Isei Tanida

Article

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mWasabi

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pORE303N_CEN12RRFuzzy3MYB

Plasmid

#194441

Purpose

CRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout: CEN12, RR, and Fuzzy3MYB

Depositor

Chung-Jui Tsai

Article

Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

Insert

gRNA array targeting CEN1, RR, Fuzzy3MYB

Use

CRISPR

Expression

Plant

Promoter

MtU6

Available Since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Cerulean_Nanotag_91

Plasmid

#130539

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_Citrine_Nanotag_23_neg_Control

Plasmid

#130562

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

negative oligo sequence

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ496

Plasmid

#44488

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

500bp Mos1, unc-119, fosmid cassette

Use

Fosmid recombineering cassette

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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FLAG-Amida

Plasmid

#15362

Depositor

Joan Conaway, Ronald Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

TCF3 (E2A) fusion partner (TFPT Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pRL1124

Plasmid

#70690

Purpose

provides avaiM, eco47iiM, and ecot22iM that encode specific methylases to enable transfer of plasmids from E. coli to Anabaena sp. strain PCC 7120 and other strains. lacks a mobilization function.

Depositor

Peter Wolk

Article

Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

Inserts

avaiM
eco47iiM
ecot22iM

Use

Bacterial shuttle vector

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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BAT-lux

Plasmid

#20890

Depositor

Stefano Piccolo

Article

Maretto et al Proc Natl Acad Sci U S A. 2003 Mar 18. 1

Insert

7xTCF binding sites, Siamois minimal promoter

Use

Luciferase

Expression

Mammalian

Mutation

7 multimerized TCF-binding elements fused upstrea…

Available Since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
mTFP1-VSVG-16

Plasmid

#55517

Purpose

Localization: Golgi/ER Transport, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

VSVG

Tags

mTFP1

Expression

Mammalian

Mutation

deltaK VSVG ts045

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA-NES-ZapCmR1.1

Plasmid

#59014

Purpose

Cytosolic zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

Insert

NES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor

Tags

Nuclear Exclusion Signal

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only